Evaluation of human bone marrow mesenchymal stem cells in the treatment of non obstructive azoospermia
Background: Non-obstructive azoospermia (NOA) represents an infertility problem that is usually difficult to treat. Such patients usually have testicular biopsy of germ cell aplasia or spermatogenic arrest. In recent decades, Mesenchymal Stem Cells (MSCs) had been studied thoroughly and proved safe...
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PAGEPress Publications
2024-03-01
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| Series: | Archivio Italiano di Urologia e Andrologia |
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| Online Access: | https://www.pagepressjournals.org/index.php/aiua/article/view/12285 |
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| author | Mohamed A. Alhefnawy Gamal Elmorsy Sayed Bakry Hesham El-amrosy Ibrahim Mearaj Ebrahim A. Sabra Osama M. Badr Dalia Ibraheem Taymour Khalifa |
| author_facet | Mohamed A. Alhefnawy Gamal Elmorsy Sayed Bakry Hesham El-amrosy Ibrahim Mearaj Ebrahim A. Sabra Osama M. Badr Dalia Ibraheem Taymour Khalifa |
| author_sort | Mohamed A. Alhefnawy |
| collection | DOAJ |
| description |
Background: Non-obstructive azoospermia (NOA) represents an infertility problem that is usually difficult to treat. Such patients usually have testicular biopsy of germ cell aplasia or spermatogenic arrest. In recent decades, Mesenchymal Stem Cells (MSCs) had been studied thoroughly and proved safe and effective regarding their capability for trans-differentiation into different cell types. The aim of this study was to evaluate the effect of MSCs local intratesticular injection in induction of spermatogenesis. Patients and method: The current study included 87 infertile non-obstructive azoospermic patients. Clinical assessment and repeated semen analysis with centrifugation were done to confirm azoospermia. Karyotyping and AZF study were done. Some of the patients had previous testicular biopsy proving a lack of sperm in the testes. Single intratesticular injection of purified MSCs suspension was done. Results: 20.7% of patients showed sperm in their semen after variable period of time. Hormonal profile among treated patients showed significant improvement regardless success of treatment. Also most of the treated patients appreciated the improvement of their sexual function and libido. Conclusions: Bone marrow derived MSCs could be a new hope and therapeutic modality for treatment of refractory cases of NOA.
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| first_indexed | 2024-04-25T01:44:01Z |
| format | Article |
| id | doaj.art-1e5c87dea61145448023c66492c2a083 |
| institution | Directory Open Access Journal |
| issn | 1124-3562 2282-4197 |
| language | English |
| last_indexed | 2024-04-25T01:44:01Z |
| publishDate | 2024-03-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Archivio Italiano di Urologia e Andrologia |
| spelling | doaj.art-1e5c87dea61145448023c66492c2a0832024-03-07T23:11:56ZengPAGEPress PublicationsArchivio Italiano di Urologia e Andrologia1124-35622282-41972024-03-0110.4081/aiua.2024.12285Evaluation of human bone marrow mesenchymal stem cells in the treatment of non obstructive azoospermiaMohamed A. Alhefnawy0Gamal Elmorsy1Sayed Bakry2Hesham El-amrosy3Ibrahim Mearaj4Ebrahim A. Sabra5Osama M. Badr6Dalia Ibraheem7Taymour Khalifa8Urology, Benha UniversityClinical Pathology, Faculty of Medicine, Al-azhar UniversityGenetic Engineering, Faculty of Science for Boys in Cairo, Al-Azhar UniversityClinical Pathology, Egypt Ministry of Health and PopulationDermatology and Andrology, Al-Azhar University Faculty of MedicineAnimal cell and tissue culture, Genetic engineering and Biotechnology Institute, Sadat UniversityAnimal cell and tissue culture, Genetic engineering and Biotechnology Institute, Sadat UniversityDepartment of Tissue engineering, Faculty of Science, Al-Azhar UniversityProfessor of Dermatologyand Andrology , Al-Azhar University Faculty of Medicine, Egypt Background: Non-obstructive azoospermia (NOA) represents an infertility problem that is usually difficult to treat. Such patients usually have testicular biopsy of germ cell aplasia or spermatogenic arrest. In recent decades, Mesenchymal Stem Cells (MSCs) had been studied thoroughly and proved safe and effective regarding their capability for trans-differentiation into different cell types. The aim of this study was to evaluate the effect of MSCs local intratesticular injection in induction of spermatogenesis. Patients and method: The current study included 87 infertile non-obstructive azoospermic patients. Clinical assessment and repeated semen analysis with centrifugation were done to confirm azoospermia. Karyotyping and AZF study were done. Some of the patients had previous testicular biopsy proving a lack of sperm in the testes. Single intratesticular injection of purified MSCs suspension was done. Results: 20.7% of patients showed sperm in their semen after variable period of time. Hormonal profile among treated patients showed significant improvement regardless success of treatment. Also most of the treated patients appreciated the improvement of their sexual function and libido. Conclusions: Bone marrow derived MSCs could be a new hope and therapeutic modality for treatment of refractory cases of NOA. https://www.pagepressjournals.org/index.php/aiua/article/view/12285Stem cellsnon-obstructive azoospermia (NOA)spermatogenesissemen analysis |
| spellingShingle | Mohamed A. Alhefnawy Gamal Elmorsy Sayed Bakry Hesham El-amrosy Ibrahim Mearaj Ebrahim A. Sabra Osama M. Badr Dalia Ibraheem Taymour Khalifa Evaluation of human bone marrow mesenchymal stem cells in the treatment of non obstructive azoospermia Archivio Italiano di Urologia e Andrologia Stem cells non-obstructive azoospermia (NOA) spermatogenesis semen analysis |
| title | Evaluation of human bone marrow mesenchymal stem cells in the treatment of non obstructive azoospermia |
| title_full | Evaluation of human bone marrow mesenchymal stem cells in the treatment of non obstructive azoospermia |
| title_fullStr | Evaluation of human bone marrow mesenchymal stem cells in the treatment of non obstructive azoospermia |
| title_full_unstemmed | Evaluation of human bone marrow mesenchymal stem cells in the treatment of non obstructive azoospermia |
| title_short | Evaluation of human bone marrow mesenchymal stem cells in the treatment of non obstructive azoospermia |
| title_sort | evaluation of human bone marrow mesenchymal stem cells in the treatment of non obstructive azoospermia |
| topic | Stem cells non-obstructive azoospermia (NOA) spermatogenesis semen analysis |
| url | https://www.pagepressjournals.org/index.php/aiua/article/view/12285 |
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