Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium <i>Ochrobactrum quorumnocens</i> T1Kr02, Containing <span style="font-variant: small-caps">d</span>-Fucose Residues
Lipopolysaccharides (LPSs) are major components of the outer membranes of Gram-negative bacteria. In this work, the structure of the O-polysaccharide of <i>Ochrobactrum quorumnocens</i> T1Kr02 was identified by nuclear magnetic resonance (NMR), and the physical–chemical properties and bi...
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2024-02-01
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| author | Aleksandra A. Krivoruchko Evelina L. Zdorovenko Maria F. Ivanova Ekaterina E. Kostina Yulia P. Fedonenko Alexander S. Shashkov Andrey S. Dmitrenok Elizaveta A. Ul’chenko Oksana V. Tkachenko Anastasia S. Astankova Gennady L. Burygin |
| author_facet | Aleksandra A. Krivoruchko Evelina L. Zdorovenko Maria F. Ivanova Ekaterina E. Kostina Yulia P. Fedonenko Alexander S. Shashkov Andrey S. Dmitrenok Elizaveta A. Ul’chenko Oksana V. Tkachenko Anastasia S. Astankova Gennady L. Burygin |
| author_sort | Aleksandra A. Krivoruchko |
| collection | DOAJ |
| description | Lipopolysaccharides (LPSs) are major components of the outer membranes of Gram-negative bacteria. In this work, the structure of the O-polysaccharide of <i>Ochrobactrum quorumnocens</i> T1Kr02 was identified by nuclear magnetic resonance (NMR), and the physical–chemical properties and biological activity of LPS were also investigated. The NMR analysis showed that the O-polysaccharide has the following structure: →2)-β-<span style="font-variant: small-caps;">d</span>-Fuc<i>f</i>-(1→3)-β-<span style="font-variant: small-caps;">d</span>-Fuc<i>p</i>-(1→. The structure of the periplasmic glucan coextracted with LPS was established by NMR spectroscopy and chemical methods: →2)-β-<span style="font-variant: small-caps;">d</span>-Glc<i>p</i>-(1→. Non-stoichiometric modifications were identified in both polysaccharides: 50% of <span style="font-variant: small-caps;">d</span>-fucofuranose residues at position 3 were O-acetylated, and 15% of <span style="font-variant: small-caps;">d</span>-Glc<i>p</i> residues at position 6 were linked with succinate. This is the first report of a polysaccharide containing both <span style="font-variant: small-caps;">d</span>-fucopyranose and <span style="font-variant: small-caps;">d</span>-fucofuranose residues. The fatty acid analysis of the LPS showed the prevalence of 3-hydroxytetradecanoic, hexadecenoic, octadecenoic, lactobacillic, and 27-hydroxyoctacosanoic acids. The dynamic light scattering demonstrated that LPS (in an aqueous solution) formed supramolecular particles with a size of 72.2 nm and a zeta-potential of –21.5 mV. The LPS solution (10 mkg/mL) promoted the growth of potato microplants under in vitro conditions. Thus, LPS of <i>O. quorumnocens</i> T1Kr02 can be recommended as a promoter for plants and as a source of biotechnological production of <span style="font-variant: small-caps;">d</span>-fucose. |
| first_indexed | 2024-03-07T22:29:24Z |
| format | Article |
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| issn | 1661-6596 1422-0067 |
| language | English |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1e6116ef29344984a9eb024c184b74142024-02-23T15:19:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01254197010.3390/ijms25041970Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium <i>Ochrobactrum quorumnocens</i> T1Kr02, Containing <span style="font-variant: small-caps">d</span>-Fucose ResiduesAleksandra A. Krivoruchko0Evelina L. Zdorovenko1Maria F. Ivanova2Ekaterina E. Kostina3Yulia P. Fedonenko4Alexander S. Shashkov5Andrey S. Dmitrenok6Elizaveta A. Ul’chenko7Oksana V. Tkachenko8Anastasia S. Astankova9Gennady L. Burygin10Department of Organic and Bioorganic Chemistry, Institute of Chemistry, Saratov State University, 410012 Saratov, RussiaN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, RussiaDepartment of Plant Breeding, Selection, and Genetics, Faculty of Agronomy, Saratov State University of Genetics, Biotechnology and Engineering Named after N.I. Vavilov, 410012 Saratov, RussiaDepartment of Plant Breeding, Selection, and Genetics, Faculty of Agronomy, Saratov State University of Genetics, Biotechnology and Engineering Named after N.I. Vavilov, 410012 Saratov, RussiaInstitute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Scientific Centre of the Russian Academy of Sciences, 410049 Saratov, RussiaN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, RussiaN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, RussiaDepartment of Biomedical Products, Faculty of Chemical Pharmaceutical Technologies, D.I. Mendeleev University of Chemical Technology of Russia, 125047 Moscow, RussiaDepartment of Plant Breeding, Selection, and Genetics, Faculty of Agronomy, Saratov State University of Genetics, Biotechnology and Engineering Named after N.I. Vavilov, 410012 Saratov, RussiaDepartment of Organic and Bioorganic Chemistry, Institute of Chemistry, Saratov State University, 410012 Saratov, RussiaDepartment of Organic and Bioorganic Chemistry, Institute of Chemistry, Saratov State University, 410012 Saratov, RussiaLipopolysaccharides (LPSs) are major components of the outer membranes of Gram-negative bacteria. In this work, the structure of the O-polysaccharide of <i>Ochrobactrum quorumnocens</i> T1Kr02 was identified by nuclear magnetic resonance (NMR), and the physical–chemical properties and biological activity of LPS were also investigated. The NMR analysis showed that the O-polysaccharide has the following structure: →2)-β-<span style="font-variant: small-caps;">d</span>-Fuc<i>f</i>-(1→3)-β-<span style="font-variant: small-caps;">d</span>-Fuc<i>p</i>-(1→. The structure of the periplasmic glucan coextracted with LPS was established by NMR spectroscopy and chemical methods: →2)-β-<span style="font-variant: small-caps;">d</span>-Glc<i>p</i>-(1→. Non-stoichiometric modifications were identified in both polysaccharides: 50% of <span style="font-variant: small-caps;">d</span>-fucofuranose residues at position 3 were O-acetylated, and 15% of <span style="font-variant: small-caps;">d</span>-Glc<i>p</i> residues at position 6 were linked with succinate. This is the first report of a polysaccharide containing both <span style="font-variant: small-caps;">d</span>-fucopyranose and <span style="font-variant: small-caps;">d</span>-fucofuranose residues. The fatty acid analysis of the LPS showed the prevalence of 3-hydroxytetradecanoic, hexadecenoic, octadecenoic, lactobacillic, and 27-hydroxyoctacosanoic acids. The dynamic light scattering demonstrated that LPS (in an aqueous solution) formed supramolecular particles with a size of 72.2 nm and a zeta-potential of –21.5 mV. The LPS solution (10 mkg/mL) promoted the growth of potato microplants under in vitro conditions. Thus, LPS of <i>O. quorumnocens</i> T1Kr02 can be recommended as a promoter for plants and as a source of biotechnological production of <span style="font-variant: small-caps;">d</span>-fucose.https://www.mdpi.com/1422-0067/25/4/1970<i>Ochrobactrum quorumnocens</i> T1Kr02lipopolysaccharideO-polysaccharide structure<span style="font-variant: small-caps">d</span>-fucoseβ-glucanesuccinate |
| spellingShingle | Aleksandra A. Krivoruchko Evelina L. Zdorovenko Maria F. Ivanova Ekaterina E. Kostina Yulia P. Fedonenko Alexander S. Shashkov Andrey S. Dmitrenok Elizaveta A. Ul’chenko Oksana V. Tkachenko Anastasia S. Astankova Gennady L. Burygin Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium <i>Ochrobactrum quorumnocens</i> T1Kr02, Containing <span style="font-variant: small-caps">d</span>-Fucose Residues International Journal of Molecular Sciences <i>Ochrobactrum quorumnocens</i> T1Kr02 lipopolysaccharide O-polysaccharide structure <span style="font-variant: small-caps">d</span>-fucose β-glucane succinate |
| title | Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium <i>Ochrobactrum quorumnocens</i> T1Kr02, Containing <span style="font-variant: small-caps">d</span>-Fucose Residues |
| title_full | Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium <i>Ochrobactrum quorumnocens</i> T1Kr02, Containing <span style="font-variant: small-caps">d</span>-Fucose Residues |
| title_fullStr | Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium <i>Ochrobactrum quorumnocens</i> T1Kr02, Containing <span style="font-variant: small-caps">d</span>-Fucose Residues |
| title_full_unstemmed | Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium <i>Ochrobactrum quorumnocens</i> T1Kr02, Containing <span style="font-variant: small-caps">d</span>-Fucose Residues |
| title_short | Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium <i>Ochrobactrum quorumnocens</i> T1Kr02, Containing <span style="font-variant: small-caps">d</span>-Fucose Residues |
| title_sort | structure physicochemical properties and biological activity of lipopolysaccharide from the rhizospheric bacterium i ochrobactrum quorumnocens i t1kr02 containing span style font variant small caps d span fucose residues |
| topic | <i>Ochrobactrum quorumnocens</i> T1Kr02 lipopolysaccharide O-polysaccharide structure <span style="font-variant: small-caps">d</span>-fucose β-glucane succinate |
| url | https://www.mdpi.com/1422-0067/25/4/1970 |
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