Cold Exposure Affects Lipid Metabolism, Fatty Acids Composition and Transcription in Pig Skeletal Muscle
Cold exposure promotes glucose oxidation and modulates the lipid metabolism in adipose tissue, but it is still not fully clear whether cold exposure could affect meat quality and fatty acid metabolism in skeletal muscle of pig in vivo. Here, we kept finishing pigs under cold or room temperature over...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2021.748801/full |
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| author | Ziye Xu Ziye Xu Ziye Xu Wentao Chen Wentao Chen Wentao Chen Liyi Wang Liyi Wang Liyi Wang Yanbing Zhou Yanbing Zhou Yanbing Zhou Qiuyun Nong Qiuyun Nong Qiuyun Nong Teresa G. Valencak Yizhen Wang Yizhen Wang Yizhen Wang Jintang Xie Tizhong Shan Tizhong Shan Tizhong Shan |
| author_facet | Ziye Xu Ziye Xu Ziye Xu Wentao Chen Wentao Chen Wentao Chen Liyi Wang Liyi Wang Liyi Wang Yanbing Zhou Yanbing Zhou Yanbing Zhou Qiuyun Nong Qiuyun Nong Qiuyun Nong Teresa G. Valencak Yizhen Wang Yizhen Wang Yizhen Wang Jintang Xie Tizhong Shan Tizhong Shan Tizhong Shan |
| author_sort | Ziye Xu |
| collection | DOAJ |
| description | Cold exposure promotes glucose oxidation and modulates the lipid metabolism in adipose tissue, but it is still not fully clear whether cold exposure could affect meat quality and fatty acid metabolism in skeletal muscle of pig in vivo. Here, we kept finishing pigs under cold or room temperature overnight and determined the effects of cold exposure on meat quality, fatty acids composition and transcriptional changes in skeletal muscle of pigs. We found that cold exposure significantly reduced the meat colour24 h and pH24 h, without affecting carcass characteristics and other meat quality traits. Considerable changes were found in the proportions of individual fatty acids and the total content of saturated fatty acid, polyunsaturated fatty acids, monounsaturated fatty acid and n3-fatty acids. RNA-seq results showed upregulated fatty acid biosynthesis genes and downregulated mitochondrial beta-oxidation genes. The lipid metabolism in cold-treated longissimus dorsi muscle might be regulated by functions of the lipoprotein particle, the extracellular matrix, and the PPAR signaling pathways. Our study revealed the potential of cold exposure to regulate the lipid metabolism and fatty acid composition in skeletal muscle of farmed animals. |
| first_indexed | 2024-12-20T02:43:22Z |
| format | Article |
| id | doaj.art-1e61c28e1d4a4641be2a6e845a341c40 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-20T02:43:22Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-1e61c28e1d4a4641be2a6e845a341c402022-12-21T19:56:14ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-10-011210.3389/fphys.2021.748801748801Cold Exposure Affects Lipid Metabolism, Fatty Acids Composition and Transcription in Pig Skeletal MuscleZiye Xu0Ziye Xu1Ziye Xu2Wentao Chen3Wentao Chen4Wentao Chen5Liyi Wang6Liyi Wang7Liyi Wang8Yanbing Zhou9Yanbing Zhou10Yanbing Zhou11Qiuyun Nong12Qiuyun Nong13Qiuyun Nong14Teresa G. Valencak15Yizhen Wang16Yizhen Wang17Yizhen Wang18Jintang Xie19Tizhong Shan20Tizhong Shan21Tizhong Shan22College of Animal Sciences, Zhejiang University, Hangzhou, ChinaKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, ChinaKey Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou, ChinaKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, ChinaKey Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou, ChinaKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, ChinaKey Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou, ChinaKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, ChinaKey Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou, ChinaKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, ChinaKey Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou, ChinaKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, ChinaKey Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou, ChinaShandong Chunteng Food Co., Ltd., Zaozhuang, ChinaCollege of Animal Sciences, Zhejiang University, Hangzhou, ChinaKey Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, Hangzhou, ChinaKey Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou, ChinaCold exposure promotes glucose oxidation and modulates the lipid metabolism in adipose tissue, but it is still not fully clear whether cold exposure could affect meat quality and fatty acid metabolism in skeletal muscle of pig in vivo. Here, we kept finishing pigs under cold or room temperature overnight and determined the effects of cold exposure on meat quality, fatty acids composition and transcriptional changes in skeletal muscle of pigs. We found that cold exposure significantly reduced the meat colour24 h and pH24 h, without affecting carcass characteristics and other meat quality traits. Considerable changes were found in the proportions of individual fatty acids and the total content of saturated fatty acid, polyunsaturated fatty acids, monounsaturated fatty acid and n3-fatty acids. RNA-seq results showed upregulated fatty acid biosynthesis genes and downregulated mitochondrial beta-oxidation genes. The lipid metabolism in cold-treated longissimus dorsi muscle might be regulated by functions of the lipoprotein particle, the extracellular matrix, and the PPAR signaling pathways. Our study revealed the potential of cold exposure to regulate the lipid metabolism and fatty acid composition in skeletal muscle of farmed animals.https://www.frontiersin.org/articles/10.3389/fphys.2021.748801/fullcold exposuremeat qualityfatty acidtranscriptomepigskeletal muscle |
| spellingShingle | Ziye Xu Ziye Xu Ziye Xu Wentao Chen Wentao Chen Wentao Chen Liyi Wang Liyi Wang Liyi Wang Yanbing Zhou Yanbing Zhou Yanbing Zhou Qiuyun Nong Qiuyun Nong Qiuyun Nong Teresa G. Valencak Yizhen Wang Yizhen Wang Yizhen Wang Jintang Xie Tizhong Shan Tizhong Shan Tizhong Shan Cold Exposure Affects Lipid Metabolism, Fatty Acids Composition and Transcription in Pig Skeletal Muscle Frontiers in Physiology cold exposure meat quality fatty acid transcriptome pig skeletal muscle |
| title | Cold Exposure Affects Lipid Metabolism, Fatty Acids Composition and Transcription in Pig Skeletal Muscle |
| title_full | Cold Exposure Affects Lipid Metabolism, Fatty Acids Composition and Transcription in Pig Skeletal Muscle |
| title_fullStr | Cold Exposure Affects Lipid Metabolism, Fatty Acids Composition and Transcription in Pig Skeletal Muscle |
| title_full_unstemmed | Cold Exposure Affects Lipid Metabolism, Fatty Acids Composition and Transcription in Pig Skeletal Muscle |
| title_short | Cold Exposure Affects Lipid Metabolism, Fatty Acids Composition and Transcription in Pig Skeletal Muscle |
| title_sort | cold exposure affects lipid metabolism fatty acids composition and transcription in pig skeletal muscle |
| topic | cold exposure meat quality fatty acid transcriptome pig skeletal muscle |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2021.748801/full |
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