OPDA has key role in regulating plant susceptibility to the root-knot nematode Meloidogyne hapla in Arabidopsis
Jasmonic acid (JA) is a plant hormone that plays important roles in regulating plant defenses against necrotrophic pathogens and herbivorous insects, but the role of JA in mediating the plant responses to root-knot nematodes has been unclear. Here we show that an application of either methyl jasmona...
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Frontiers Media S.A.
2016-10-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01565/full |
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| author | Cynthia Gleason Cynthia Gleason Natthanon Leelarasamee Dorothea Meldau Ivo Feussner Ivo Feussner |
| author_facet | Cynthia Gleason Cynthia Gleason Natthanon Leelarasamee Dorothea Meldau Ivo Feussner Ivo Feussner |
| author_sort | Cynthia Gleason |
| collection | DOAJ |
| description | Jasmonic acid (JA) is a plant hormone that plays important roles in regulating plant defenses against necrotrophic pathogens and herbivorous insects, but the role of JA in mediating the plant responses to root-knot nematodes has been unclear. Here we show that an application of either methyl jasmonate (MeJA) or the JA-mimic coronatine (COR) on Arabidopsis significantly reduced the number of galls caused by the root-knot nematode Meloidogyne hapla. Interestingly, the MeJA-induced resistance was independent of the JA-receptor COI1 (CORONATINE INSENSITIVE 1). The MeJA-treated plants accumulated the JA precursor cis-(+)-12-oxo-phytodienoic acid (OPDA) in addition to JA/JA-Isoleucine, indicating a positive feedback loop in JA biosynthesis. Using mutants in the JA-biosynthetic pathway, we found that plants deficient in the biosynthesis of JA and OPDA were hyper-susceptible to M. hapla. However, the opr3 mutant, which cannot convert OPDA to JA, exhibited wild-type levels of nematode galling. In addition, mutants in the JA-biosynthesis and perception which lie downstream of opr3 also displayed wild-type levels of galling. The data puts OPR3 (OPDA reductase 3) as the branch point between hyper-susceptibility and wild-type like levels of disease. Overall, the data suggests that the JA precursor, OPDA, plays a role in regulating plant defense against nematodes. |
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| issn | 1664-462X |
| language | English |
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| publishDate | 2016-10-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-1e64bb27781c4e72bdaf9d0af6374bff2022-12-22T00:44:48ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-10-01710.3389/fpls.2016.01565224933OPDA has key role in regulating plant susceptibility to the root-knot nematode Meloidogyne hapla in ArabidopsisCynthia Gleason0Cynthia Gleason1Natthanon Leelarasamee2Dorothea Meldau3Ivo Feussner4Ivo Feussner5Georg August UniversityGeorg August UniversityGeorg August UniversityGeorg August UniversityGeorg August UniversityGeorg August UniversityJasmonic acid (JA) is a plant hormone that plays important roles in regulating plant defenses against necrotrophic pathogens and herbivorous insects, but the role of JA in mediating the plant responses to root-knot nematodes has been unclear. Here we show that an application of either methyl jasmonate (MeJA) or the JA-mimic coronatine (COR) on Arabidopsis significantly reduced the number of galls caused by the root-knot nematode Meloidogyne hapla. Interestingly, the MeJA-induced resistance was independent of the JA-receptor COI1 (CORONATINE INSENSITIVE 1). The MeJA-treated plants accumulated the JA precursor cis-(+)-12-oxo-phytodienoic acid (OPDA) in addition to JA/JA-Isoleucine, indicating a positive feedback loop in JA biosynthesis. Using mutants in the JA-biosynthetic pathway, we found that plants deficient in the biosynthesis of JA and OPDA were hyper-susceptible to M. hapla. However, the opr3 mutant, which cannot convert OPDA to JA, exhibited wild-type levels of nematode galling. In addition, mutants in the JA-biosynthesis and perception which lie downstream of opr3 also displayed wild-type levels of galling. The data puts OPR3 (OPDA reductase 3) as the branch point between hyper-susceptibility and wild-type like levels of disease. Overall, the data suggests that the JA precursor, OPDA, plays a role in regulating plant defense against nematodes.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01565/fullinduced resistanceJasmonic acidNematodesplant defenseplant hormonesopda |
| spellingShingle | Cynthia Gleason Cynthia Gleason Natthanon Leelarasamee Dorothea Meldau Ivo Feussner Ivo Feussner OPDA has key role in regulating plant susceptibility to the root-knot nematode Meloidogyne hapla in Arabidopsis Frontiers in Plant Science induced resistance Jasmonic acid Nematodes plant defense plant hormones opda |
| title | OPDA has key role in regulating plant susceptibility to the root-knot nematode Meloidogyne hapla in Arabidopsis |
| title_full | OPDA has key role in regulating plant susceptibility to the root-knot nematode Meloidogyne hapla in Arabidopsis |
| title_fullStr | OPDA has key role in regulating plant susceptibility to the root-knot nematode Meloidogyne hapla in Arabidopsis |
| title_full_unstemmed | OPDA has key role in regulating plant susceptibility to the root-knot nematode Meloidogyne hapla in Arabidopsis |
| title_short | OPDA has key role in regulating plant susceptibility to the root-knot nematode Meloidogyne hapla in Arabidopsis |
| title_sort | opda has key role in regulating plant susceptibility to the root knot nematode meloidogyne hapla in arabidopsis |
| topic | induced resistance Jasmonic acid Nematodes plant defense plant hormones opda |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01565/full |
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