Development of serological assay for detection of antibodies in the N protein of SARS-CoV-2 in human sera in Mongolia
The capability to detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection and identify immune responses among the population is crucial for managing the outbreak of the COVID-19 pandemic. Although PCR-based nucleic acid detection techniques are utilized to detect viral infect...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Mongolian Academy of Sciences
2021-09-01
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| Series: | Proceedings of the Mongolian Academy of Sciences |
| Subjects: | |
| Online Access: | https://www.mongoliajol.info/index.php/PMAS/article/view/1819 |
| _version_ | 1818237749849751552 |
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| author | Gantulga Davaakhuu Zolzaya Sandag Nomin Myagmar Nomuun Oyunbat Ariya Enkhtuya Khurelsukh Buyanbat Maral Davaanyam Tuul Boldbaatar Darmaa Badarch Naranzul Tsedenbal Lkhagvasuren Damdindorj Oyunsuren Tsendsuren |
| author_facet | Gantulga Davaakhuu Zolzaya Sandag Nomin Myagmar Nomuun Oyunbat Ariya Enkhtuya Khurelsukh Buyanbat Maral Davaanyam Tuul Boldbaatar Darmaa Badarch Naranzul Tsedenbal Lkhagvasuren Damdindorj Oyunsuren Tsendsuren |
| author_sort | Gantulga Davaakhuu |
| collection | DOAJ |
| description | The capability to detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection and identify immune responses among the population is crucial for managing the outbreak of the COVID-19 pandemic.
Although PCR-based nucleic acid detection techniques are utilized to detect viral infection in people, alternative tests capable of distinguishing between exposure and infection are urgently needed beyond this restricted window of detectable viral replication. Antibodies are produced in human sera within a few days after viral infection, providing longer period for performing tests to acquire reliable database.
Herewith, we provide the results of our in-house developed ELISA (Enzyme-Linked Immunosorbent Assay) that displays all of the properties necessary for high-throughput of human sera sample analysis. This test does not involve the handling of live viruses, although it detects a variety of antibody types in serum and plasma of human after exposure to the virus.
For in-house development of the kit, the nucleocapsid (N) gene of SARS-CoV-2 virus was cloned in the prokaryotic expression vector pGEX-6P-1, and purified N protein was used to detect IgG antibodies in human sera samples. In total 76 human serum samples that were collected before novel coronavirus registry in Mongolia in March 2020, as well as 200 serum samples from patients who had been infected by SARS-CoV-2 virus, were used.
Among 200 serum samples, 188 were positive and 12 were false negative, while in non-infected cases 69 were negative and 7 were false positive, suggesting 94 per cent sensitivity and 90.7 per cent specificity of the kit, with p-values of 0.02. |
| first_indexed | 2024-12-12T12:30:43Z |
| format | Article |
| id | doaj.art-1e6547d31ff64b89879b19f9592bd1ff |
| institution | Directory Open Access Journal |
| issn | 2310-4716 2312-2994 |
| language | English |
| last_indexed | 2024-12-12T12:30:43Z |
| publishDate | 2021-09-01 |
| publisher | Mongolian Academy of Sciences |
| record_format | Article |
| series | Proceedings of the Mongolian Academy of Sciences |
| spelling | doaj.art-1e6547d31ff64b89879b19f9592bd1ff2022-12-22T00:24:25ZengMongolian Academy of SciencesProceedings of the Mongolian Academy of Sciences2310-47162312-29942021-09-01162310.5564/pmas.v61i03.18191770Development of serological assay for detection of antibodies in the N protein of SARS-CoV-2 in human sera in MongoliaGantulga Davaakhuu0https://orcid.org/0000-0003-3792-7101Zolzaya Sandag1https://orcid.org/0000-0001-9901-2156Nomin Myagmar2https://orcid.org/0000-0002-3448-9137Nomuun Oyunbat3https://orcid.org/0000-0002-0081-0105Ariya Enkhtuya4https://orcid.org/0000-0002-2623-840XKhurelsukh Buyanbat5https://orcid.org/0000-0003-2441-6118Maral Davaanyam6https://orcid.org/0000-0003-1712-6446Tuul Boldbaatar7https://orcid.org/0000-0002-5127-6432Darmaa Badarch8https://orcid.org/0000-0002-7097-0745Naranzul Tsedenbal9https://orcid.org/0000-0001-6993-4678Lkhagvasuren Damdindorj10https://orcid.org/0000-0002-5052-0341Oyunsuren Tsendsuren11https://orcid.org/0000-0001-9906-2428Laboratory of Molecular Biology, Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaLaboratory of Molecular Biology, Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaLaboratory of Molecular Biology, Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaLaboratory of Molecular Biology, Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaLaboratory of Molecular Biology, Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaLaboratory of Molecular Biology, Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaLaboratory of Molecular Biology, Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaLaboratory of Molecular Biology, Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaLaboratory of Virology, National Center for Communicable Diseases, Ulaanbaatar, MongoliaLaboratory of Virology, National Center for Communicable Diseases, Ulaanbaatar, MongoliaDepartment of Biology, School of Arts and Sciences, National University of Mongolia, Ulaanbaatar, MongoliaLaboratory of Molecular Biology, Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaThe capability to detect Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection and identify immune responses among the population is crucial for managing the outbreak of the COVID-19 pandemic. Although PCR-based nucleic acid detection techniques are utilized to detect viral infection in people, alternative tests capable of distinguishing between exposure and infection are urgently needed beyond this restricted window of detectable viral replication. Antibodies are produced in human sera within a few days after viral infection, providing longer period for performing tests to acquire reliable database. Herewith, we provide the results of our in-house developed ELISA (Enzyme-Linked Immunosorbent Assay) that displays all of the properties necessary for high-throughput of human sera sample analysis. This test does not involve the handling of live viruses, although it detects a variety of antibody types in serum and plasma of human after exposure to the virus. For in-house development of the kit, the nucleocapsid (N) gene of SARS-CoV-2 virus was cloned in the prokaryotic expression vector pGEX-6P-1, and purified N protein was used to detect IgG antibodies in human sera samples. In total 76 human serum samples that were collected before novel coronavirus registry in Mongolia in March 2020, as well as 200 serum samples from patients who had been infected by SARS-CoV-2 virus, were used. Among 200 serum samples, 188 were positive and 12 were false negative, while in non-infected cases 69 were negative and 7 were false positive, suggesting 94 per cent sensitivity and 90.7 per cent specificity of the kit, with p-values of 0.02.https://www.mongoliajol.info/index.php/PMAS/article/view/1819sars-cov-2 diagnosiselisanucleocapsid proteinigg |
| spellingShingle | Gantulga Davaakhuu Zolzaya Sandag Nomin Myagmar Nomuun Oyunbat Ariya Enkhtuya Khurelsukh Buyanbat Maral Davaanyam Tuul Boldbaatar Darmaa Badarch Naranzul Tsedenbal Lkhagvasuren Damdindorj Oyunsuren Tsendsuren Development of serological assay for detection of antibodies in the N protein of SARS-CoV-2 in human sera in Mongolia Proceedings of the Mongolian Academy of Sciences sars-cov-2 diagnosis elisa nucleocapsid protein igg |
| title | Development of serological assay for detection of antibodies in the N protein of SARS-CoV-2 in human sera in Mongolia |
| title_full | Development of serological assay for detection of antibodies in the N protein of SARS-CoV-2 in human sera in Mongolia |
| title_fullStr | Development of serological assay for detection of antibodies in the N protein of SARS-CoV-2 in human sera in Mongolia |
| title_full_unstemmed | Development of serological assay for detection of antibodies in the N protein of SARS-CoV-2 in human sera in Mongolia |
| title_short | Development of serological assay for detection of antibodies in the N protein of SARS-CoV-2 in human sera in Mongolia |
| title_sort | development of serological assay for detection of antibodies in the n protein of sars cov 2 in human sera in mongolia |
| topic | sars-cov-2 diagnosis elisa nucleocapsid protein igg |
| url | https://www.mongoliajol.info/index.php/PMAS/article/view/1819 |
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