Standard Methods for Dissection of <i>Varroa destructor</i> Females

<i>Varroa destructor</i> (Anderson and Trueman) is known as a major pest of <i>Apis mellifera</i> L, especially in the Northern Hemisphere where its effects can be deleterious. As an obligate parasite, this mite relies entirely on its host to reproduce and complete its cycle....

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Main Authors: Vincent Piou, Caroline Vilarem, Carolin Rein, Lina Sprau, Angélique Vétillard
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Insects
Subjects:
Online Access:https://www.mdpi.com/2075-4450/13/1/37
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author Vincent Piou
Caroline Vilarem
Carolin Rein
Lina Sprau
Angélique Vétillard
author_facet Vincent Piou
Caroline Vilarem
Carolin Rein
Lina Sprau
Angélique Vétillard
author_sort Vincent Piou
collection DOAJ
description <i>Varroa destructor</i> (Anderson and Trueman) is known as a major pest of <i>Apis mellifera</i> L, especially in the Northern Hemisphere where its effects can be deleterious. As an obligate parasite, this mite relies entirely on its host to reproduce and complete its cycle. Studies focusing on isolated organs are needed to better comprehend this organism. To conduct such targeted molecular or physiological studies, the dissection of <i>V. destructor</i> mites is crucial as it allows the extraction of specific organs. Here, we propose a technical article showing detailed steps of females <i>V. destructor</i> dissection, illustrated with pictures and videos. These illustrated guidelines will represent a helpful tool to go further in <i>V. destructor</i> research.
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spelling doaj.art-1e68608214a245f58546d7e574ff1df82023-11-23T14:09:46ZengMDPI AGInsects2075-44502021-12-011313710.3390/insects13010037Standard Methods for Dissection of <i>Varroa destructor</i> FemalesVincent Piou0Caroline Vilarem1Carolin Rein2Lina Sprau3Angélique Vétillard4Laboratoire Evolution et Diversité Biologique, UMR5174, CNRS-Université de Toulouse III-IRD, INU Jean-François Champollion, Université Paul Sabatier, 31077 Toulouse, FranceLaboratoire Evolution et Diversité Biologique, UMR5174, CNRS-Université de Toulouse III-IRD, INU Jean-François Champollion, Université Paul Sabatier, 31077 Toulouse, FranceApicultural State Institute, University of Hohenheim, Erna-Hruschka-Weg 6, 70599 Stuttgart, GermanyDepartment of Livestock Population Genomics, University of Hohenheim, Garbenstr. 17, 70599 Stuttgart, GermanyLaboratoire Evolution et Diversité Biologique, UMR5174, CNRS-Université de Toulouse III-IRD, INU Jean-François Champollion, Université Paul Sabatier, 31077 Toulouse, France<i>Varroa destructor</i> (Anderson and Trueman) is known as a major pest of <i>Apis mellifera</i> L, especially in the Northern Hemisphere where its effects can be deleterious. As an obligate parasite, this mite relies entirely on its host to reproduce and complete its cycle. Studies focusing on isolated organs are needed to better comprehend this organism. To conduct such targeted molecular or physiological studies, the dissection of <i>V. destructor</i> mites is crucial as it allows the extraction of specific organs. Here, we propose a technical article showing detailed steps of females <i>V. destructor</i> dissection, illustrated with pictures and videos. These illustrated guidelines will represent a helpful tool to go further in <i>V. destructor</i> research.https://www.mdpi.com/2075-4450/13/1/37<i>Varroa destructor</i>dissectionguidelinespicturesvideos
spellingShingle Vincent Piou
Caroline Vilarem
Carolin Rein
Lina Sprau
Angélique Vétillard
Standard Methods for Dissection of <i>Varroa destructor</i> Females
Insects
<i>Varroa destructor</i>
dissection
guidelines
pictures
videos
title Standard Methods for Dissection of <i>Varroa destructor</i> Females
title_full Standard Methods for Dissection of <i>Varroa destructor</i> Females
title_fullStr Standard Methods for Dissection of <i>Varroa destructor</i> Females
title_full_unstemmed Standard Methods for Dissection of <i>Varroa destructor</i> Females
title_short Standard Methods for Dissection of <i>Varroa destructor</i> Females
title_sort standard methods for dissection of i varroa destructor i females
topic <i>Varroa destructor</i>
dissection
guidelines
pictures
videos
url https://www.mdpi.com/2075-4450/13/1/37
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