Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt
ABSTRACT: Exopolysaccharide from Leuconostoc pseudomesenteroides XG5 (XG5 EPS) is a linear dextran that is built by glucose units via α-1,6 glycosidic bond. The primary objective of this study was to investigate the yield of XG5 EPS and its application in set yogurt. In laboratory scale, the culture...
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Elsevier
2022-02-01
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Series: | Journal of Dairy Science |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030221011188 |
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author | Lei Pan Qi Wang Liangfan Qu Lu Liang Ye Han Xianghe Wang Zhijiang Zhou |
author_facet | Lei Pan Qi Wang Liangfan Qu Lu Liang Ye Han Xianghe Wang Zhijiang Zhou |
author_sort | Lei Pan |
collection | DOAJ |
description | ABSTRACT: Exopolysaccharide from Leuconostoc pseudomesenteroides XG5 (XG5 EPS) is a linear dextran that is built by glucose units via α-1,6 glycosidic bond. The primary objective of this study was to investigate the yield of XG5 EPS and its application in set yogurt. In laboratory scale, the culture conditions of XG5 EPS production were optimized using the L9 (33) orthogonal test. Here, the optimized yield of XG5 EPS was 26.02 g/L under the conditions of 100 g/L sucrose, initial pH 7.0, 25°C incubation, and 100 rpm for 36 h in a shaking flask. Based on the optimized parameters of laboratory scale, a pilot fed-batch fermentation was performed in a 50-L bioreactor with an adjusted agitation speed of 20 rpm. The XG5 EPS yield reached 40.07 g/L in fed-batch fermentation, which was 54% higher than that achieved in laboratory scale. In addition, XG5 EPS was added into set yogurt to investigate its effect on the stability of set yogurt. Our data demonstrated that the XG5 EPS improved the water-holding capacity, texture profile, and viscosity of set yogurt during cold storage compared with the controls. In particular, addition of 0.5% XG5 EPS increased the structure of 3-dimensional network of set yogurt, which eventually improved the physical stability of the set yogurt. Overall, this study provided new insights for exploring the pilot scale production and application of dextran. |
first_indexed | 2024-04-13T16:11:35Z |
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id | doaj.art-1e6861b03bea461892f7184b4a146f36 |
institution | Directory Open Access Journal |
issn | 0022-0302 |
language | English |
last_indexed | 2024-04-13T16:11:35Z |
publishDate | 2022-02-01 |
publisher | Elsevier |
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series | Journal of Dairy Science |
spelling | doaj.art-1e6861b03bea461892f7184b4a146f362022-12-22T02:40:14ZengElsevierJournal of Dairy Science0022-03022022-02-01105210721083Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurtLei Pan0Qi Wang1Liangfan Qu2Lu Liang3Ye Han4Xianghe Wang5Zhijiang Zhou6School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaTianjin Research Institute of Industrial Microbiology Co., Ltd., Tianjin 300462, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaTianjin Research Institute of Industrial Microbiology Co., Ltd., Tianjin 300462, China; Tianjin SF-Bio Industrial Bio-Tec Co., Ltd., Tianjin 300462, China; Corresponding authorsSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China; Corresponding authorsABSTRACT: Exopolysaccharide from Leuconostoc pseudomesenteroides XG5 (XG5 EPS) is a linear dextran that is built by glucose units via α-1,6 glycosidic bond. The primary objective of this study was to investigate the yield of XG5 EPS and its application in set yogurt. In laboratory scale, the culture conditions of XG5 EPS production were optimized using the L9 (33) orthogonal test. Here, the optimized yield of XG5 EPS was 26.02 g/L under the conditions of 100 g/L sucrose, initial pH 7.0, 25°C incubation, and 100 rpm for 36 h in a shaking flask. Based on the optimized parameters of laboratory scale, a pilot fed-batch fermentation was performed in a 50-L bioreactor with an adjusted agitation speed of 20 rpm. The XG5 EPS yield reached 40.07 g/L in fed-batch fermentation, which was 54% higher than that achieved in laboratory scale. In addition, XG5 EPS was added into set yogurt to investigate its effect on the stability of set yogurt. Our data demonstrated that the XG5 EPS improved the water-holding capacity, texture profile, and viscosity of set yogurt during cold storage compared with the controls. In particular, addition of 0.5% XG5 EPS increased the structure of 3-dimensional network of set yogurt, which eventually improved the physical stability of the set yogurt. Overall, this study provided new insights for exploring the pilot scale production and application of dextran.http://www.sciencedirect.com/science/article/pii/S0022030221011188Leuconostoc pseudomesenteroides XG5exopolysaccharidedextranfed-batch fermentationset yogurt |
spellingShingle | Lei Pan Qi Wang Liangfan Qu Lu Liang Ye Han Xianghe Wang Zhijiang Zhou Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt Journal of Dairy Science Leuconostoc pseudomesenteroides XG5 exopolysaccharide dextran fed-batch fermentation set yogurt |
title | Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt |
title_full | Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt |
title_fullStr | Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt |
title_full_unstemmed | Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt |
title_short | Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt |
title_sort | pilot scale production of exopolysaccharide from leuconostoc pseudomesenteroides xg5 and its application in set yogurt |
topic | Leuconostoc pseudomesenteroides XG5 exopolysaccharide dextran fed-batch fermentation set yogurt |
url | http://www.sciencedirect.com/science/article/pii/S0022030221011188 |
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