Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt

ABSTRACT: Exopolysaccharide from Leuconostoc pseudomesenteroides XG5 (XG5 EPS) is a linear dextran that is built by glucose units via α-1,6 glycosidic bond. The primary objective of this study was to investigate the yield of XG5 EPS and its application in set yogurt. In laboratory scale, the culture...

Full description

Bibliographic Details
Main Authors: Lei Pan, Qi Wang, Liangfan Qu, Lu Liang, Ye Han, Xianghe Wang, Zhijiang Zhou
Format: Article
Language:English
Published: Elsevier 2022-02-01
Series:Journal of Dairy Science
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022030221011188
_version_ 1811330947154444288
author Lei Pan
Qi Wang
Liangfan Qu
Lu Liang
Ye Han
Xianghe Wang
Zhijiang Zhou
author_facet Lei Pan
Qi Wang
Liangfan Qu
Lu Liang
Ye Han
Xianghe Wang
Zhijiang Zhou
author_sort Lei Pan
collection DOAJ
description ABSTRACT: Exopolysaccharide from Leuconostoc pseudomesenteroides XG5 (XG5 EPS) is a linear dextran that is built by glucose units via α-1,6 glycosidic bond. The primary objective of this study was to investigate the yield of XG5 EPS and its application in set yogurt. In laboratory scale, the culture conditions of XG5 EPS production were optimized using the L9 (33) orthogonal test. Here, the optimized yield of XG5 EPS was 26.02 g/L under the conditions of 100 g/L sucrose, initial pH 7.0, 25°C incubation, and 100 rpm for 36 h in a shaking flask. Based on the optimized parameters of laboratory scale, a pilot fed-batch fermentation was performed in a 50-L bioreactor with an adjusted agitation speed of 20 rpm. The XG5 EPS yield reached 40.07 g/L in fed-batch fermentation, which was 54% higher than that achieved in laboratory scale. In addition, XG5 EPS was added into set yogurt to investigate its effect on the stability of set yogurt. Our data demonstrated that the XG5 EPS improved the water-holding capacity, texture profile, and viscosity of set yogurt during cold storage compared with the controls. In particular, addition of 0.5% XG5 EPS increased the structure of 3-dimensional network of set yogurt, which eventually improved the physical stability of the set yogurt. Overall, this study provided new insights for exploring the pilot scale production and application of dextran.
first_indexed 2024-04-13T16:11:35Z
format Article
id doaj.art-1e6861b03bea461892f7184b4a146f36
institution Directory Open Access Journal
issn 0022-0302
language English
last_indexed 2024-04-13T16:11:35Z
publishDate 2022-02-01
publisher Elsevier
record_format Article
series Journal of Dairy Science
spelling doaj.art-1e6861b03bea461892f7184b4a146f362022-12-22T02:40:14ZengElsevierJournal of Dairy Science0022-03022022-02-01105210721083Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurtLei Pan0Qi Wang1Liangfan Qu2Lu Liang3Ye Han4Xianghe Wang5Zhijiang Zhou6School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaTianjin Research Institute of Industrial Microbiology Co., Ltd., Tianjin 300462, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, ChinaTianjin Research Institute of Industrial Microbiology Co., Ltd., Tianjin 300462, China; Tianjin SF-Bio Industrial Bio-Tec Co., Ltd., Tianjin 300462, China; Corresponding authorsSchool of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China; Corresponding authorsABSTRACT: Exopolysaccharide from Leuconostoc pseudomesenteroides XG5 (XG5 EPS) is a linear dextran that is built by glucose units via α-1,6 glycosidic bond. The primary objective of this study was to investigate the yield of XG5 EPS and its application in set yogurt. In laboratory scale, the culture conditions of XG5 EPS production were optimized using the L9 (33) orthogonal test. Here, the optimized yield of XG5 EPS was 26.02 g/L under the conditions of 100 g/L sucrose, initial pH 7.0, 25°C incubation, and 100 rpm for 36 h in a shaking flask. Based on the optimized parameters of laboratory scale, a pilot fed-batch fermentation was performed in a 50-L bioreactor with an adjusted agitation speed of 20 rpm. The XG5 EPS yield reached 40.07 g/L in fed-batch fermentation, which was 54% higher than that achieved in laboratory scale. In addition, XG5 EPS was added into set yogurt to investigate its effect on the stability of set yogurt. Our data demonstrated that the XG5 EPS improved the water-holding capacity, texture profile, and viscosity of set yogurt during cold storage compared with the controls. In particular, addition of 0.5% XG5 EPS increased the structure of 3-dimensional network of set yogurt, which eventually improved the physical stability of the set yogurt. Overall, this study provided new insights for exploring the pilot scale production and application of dextran.http://www.sciencedirect.com/science/article/pii/S0022030221011188Leuconostoc pseudomesenteroides XG5exopolysaccharidedextranfed-batch fermentationset yogurt
spellingShingle Lei Pan
Qi Wang
Liangfan Qu
Lu Liang
Ye Han
Xianghe Wang
Zhijiang Zhou
Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt
Journal of Dairy Science
Leuconostoc pseudomesenteroides XG5
exopolysaccharide
dextran
fed-batch fermentation
set yogurt
title Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt
title_full Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt
title_fullStr Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt
title_full_unstemmed Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt
title_short Pilot-scale production of exopolysaccharide from Leuconostoc pseudomesenteroides XG5 and its application in set yogurt
title_sort pilot scale production of exopolysaccharide from leuconostoc pseudomesenteroides xg5 and its application in set yogurt
topic Leuconostoc pseudomesenteroides XG5
exopolysaccharide
dextran
fed-batch fermentation
set yogurt
url http://www.sciencedirect.com/science/article/pii/S0022030221011188
work_keys_str_mv AT leipan pilotscaleproductionofexopolysaccharidefromleuconostocpseudomesenteroidesxg5anditsapplicationinsetyogurt
AT qiwang pilotscaleproductionofexopolysaccharidefromleuconostocpseudomesenteroidesxg5anditsapplicationinsetyogurt
AT liangfanqu pilotscaleproductionofexopolysaccharidefromleuconostocpseudomesenteroidesxg5anditsapplicationinsetyogurt
AT luliang pilotscaleproductionofexopolysaccharidefromleuconostocpseudomesenteroidesxg5anditsapplicationinsetyogurt
AT yehan pilotscaleproductionofexopolysaccharidefromleuconostocpseudomesenteroidesxg5anditsapplicationinsetyogurt
AT xianghewang pilotscaleproductionofexopolysaccharidefromleuconostocpseudomesenteroidesxg5anditsapplicationinsetyogurt
AT zhijiangzhou pilotscaleproductionofexopolysaccharidefromleuconostocpseudomesenteroidesxg5anditsapplicationinsetyogurt