Using iron sucrose-labeled adipose-derived mesenchymal stem cells in 1.5 and 3 T MRI tracking: An in vitro study
Objectives: The objective of this study was to investigate iron sucrose labeling in mesenchymal stem cell (MSCs) tracking. Background: Adipose-derived mesenchymal stem cell-based therapy is a promising strategy for promoting musculoskeletal repair. Methods: Iron sucrose-labeled adipose-derived mesen...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-08-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020314262 |
| _version_ | 1818201622947299328 |
|---|---|
| author | Paween Tangchitphisut Narongrit Srikaew Sith Phongkitkarun Suphaneewan Jaovisidha Tulyapruek Tawonsawatruk |
| author_facet | Paween Tangchitphisut Narongrit Srikaew Sith Phongkitkarun Suphaneewan Jaovisidha Tulyapruek Tawonsawatruk |
| author_sort | Paween Tangchitphisut |
| collection | DOAJ |
| description | Objectives: The objective of this study was to investigate iron sucrose labeling in mesenchymal stem cell (MSCs) tracking. Background: Adipose-derived mesenchymal stem cell-based therapy is a promising strategy for promoting musculoskeletal repair. Methods: Iron sucrose-labeled adipose-derived mesenchymal stem cells (IS-labeled ASCs) were tracked using T2-and T2∗-weighted sequences by 1.5 and 3 T MRI in an in vitro model. ASCs were isolated from cosmetic liposuction specimens. ASCs from passages 4–6 were labeled with iron sucrose (Venofer®) which was added to the cell culture medium. Pre- and post-iron sucrose labeled ASCs were evaluated for cell surface immunophenotypes. Cell viability as well as chondrogenic, adipogenic and osteogenic differentiation of IS-labeled-ASCs were evaluated. The IS-labeled ASCs were titrated into microtubes at 1 × 103, 1 × 104, 1 × 105 and 1 × 106 cells/ml/microtube and their intensities were determined by 1.5 and 3T MRI using T2-and T2∗-weighted sequences. Results: The expression markers of IS-labeled ASCs from flow cytometry were equivalent to control. The mean cell viability was 97.73 ± 2.06%. Cell differentiations of IS-labeled ASCs were confirmed in each lineage using specific staining solutions. T2∗-weighted sequences (T2∗) were able to detect iron sucrose labeled-ASCs at a minimum of 1 × 105 cells/ml/microtube using 1.5 and 3T MRI, but the detection sensitivity was lower with T2-weighted sequences (T2). Conclusions: Iron sucrose incubation is a safe alternative method for ASCs labeling and tracking using MRI following treatment. Clinicians and researchers should be able to visualize the location of ASCs engraftment without secondary surgical investigation involving tissue sampling. |
| first_indexed | 2024-12-12T02:56:29Z |
| format | Article |
| id | doaj.art-1e6c56bb5d6b49deb3ffafa0b948ec56 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-12T02:56:29Z |
| publishDate | 2020-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-1e6c56bb5d6b49deb3ffafa0b948ec562022-12-22T00:40:45ZengElsevierHeliyon2405-84402020-08-0168e04582Using iron sucrose-labeled adipose-derived mesenchymal stem cells in 1.5 and 3 T MRI tracking: An in vitro studyPaween Tangchitphisut0Narongrit Srikaew1Sith Phongkitkarun2Suphaneewan Jaovisidha3Tulyapruek Tawonsawatruk4Department of Orthopaedics, School of Medicine, Mae Fah Luang University, Thailand; Corresponding author.Research and Innovative Center, Ramathibodi Hospital, ThailandDepartment of Radiology, Faculty of Medicine, Ramathibodi Hospital, ThailandDepartment of Radiology, Faculty of Medicine, Ramathibodi Hospital, ThailandDepartment of Orthopaedics, Faculty of Medicine, Ramathibodi Hospital, ThailandObjectives: The objective of this study was to investigate iron sucrose labeling in mesenchymal stem cell (MSCs) tracking. Background: Adipose-derived mesenchymal stem cell-based therapy is a promising strategy for promoting musculoskeletal repair. Methods: Iron sucrose-labeled adipose-derived mesenchymal stem cells (IS-labeled ASCs) were tracked using T2-and T2∗-weighted sequences by 1.5 and 3 T MRI in an in vitro model. ASCs were isolated from cosmetic liposuction specimens. ASCs from passages 4–6 were labeled with iron sucrose (Venofer®) which was added to the cell culture medium. Pre- and post-iron sucrose labeled ASCs were evaluated for cell surface immunophenotypes. Cell viability as well as chondrogenic, adipogenic and osteogenic differentiation of IS-labeled-ASCs were evaluated. The IS-labeled ASCs were titrated into microtubes at 1 × 103, 1 × 104, 1 × 105 and 1 × 106 cells/ml/microtube and their intensities were determined by 1.5 and 3T MRI using T2-and T2∗-weighted sequences. Results: The expression markers of IS-labeled ASCs from flow cytometry were equivalent to control. The mean cell viability was 97.73 ± 2.06%. Cell differentiations of IS-labeled ASCs were confirmed in each lineage using specific staining solutions. T2∗-weighted sequences (T2∗) were able to detect iron sucrose labeled-ASCs at a minimum of 1 × 105 cells/ml/microtube using 1.5 and 3T MRI, but the detection sensitivity was lower with T2-weighted sequences (T2). Conclusions: Iron sucrose incubation is a safe alternative method for ASCs labeling and tracking using MRI following treatment. Clinicians and researchers should be able to visualize the location of ASCs engraftment without secondary surgical investigation involving tissue sampling.http://www.sciencedirect.com/science/article/pii/S2405844020314262BioengineeringTissue engineeringStem cells researchRegenerative medicineOrthopedicsRadiology |
| spellingShingle | Paween Tangchitphisut Narongrit Srikaew Sith Phongkitkarun Suphaneewan Jaovisidha Tulyapruek Tawonsawatruk Using iron sucrose-labeled adipose-derived mesenchymal stem cells in 1.5 and 3 T MRI tracking: An in vitro study Heliyon Bioengineering Tissue engineering Stem cells research Regenerative medicine Orthopedics Radiology |
| title | Using iron sucrose-labeled adipose-derived mesenchymal stem cells in 1.5 and 3 T MRI tracking: An in vitro study |
| title_full | Using iron sucrose-labeled adipose-derived mesenchymal stem cells in 1.5 and 3 T MRI tracking: An in vitro study |
| title_fullStr | Using iron sucrose-labeled adipose-derived mesenchymal stem cells in 1.5 and 3 T MRI tracking: An in vitro study |
| title_full_unstemmed | Using iron sucrose-labeled adipose-derived mesenchymal stem cells in 1.5 and 3 T MRI tracking: An in vitro study |
| title_short | Using iron sucrose-labeled adipose-derived mesenchymal stem cells in 1.5 and 3 T MRI tracking: An in vitro study |
| title_sort | using iron sucrose labeled adipose derived mesenchymal stem cells in 1 5 and 3 t mri tracking an in vitro study |
| topic | Bioengineering Tissue engineering Stem cells research Regenerative medicine Orthopedics Radiology |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020314262 |
| work_keys_str_mv | AT paweentangchitphisut usingironsucroselabeledadiposederivedmesenchymalstemcellsin15and3tmritrackinganinvitrostudy AT narongritsrikaew usingironsucroselabeledadiposederivedmesenchymalstemcellsin15and3tmritrackinganinvitrostudy AT sithphongkitkarun usingironsucroselabeledadiposederivedmesenchymalstemcellsin15and3tmritrackinganinvitrostudy AT suphaneewanjaovisidha usingironsucroselabeledadiposederivedmesenchymalstemcellsin15and3tmritrackinganinvitrostudy AT tulyapruektawonsawatruk usingironsucroselabeledadiposederivedmesenchymalstemcellsin15and3tmritrackinganinvitrostudy |