Microfluidic Impedance Cytometry for Single‐Cell Particulate Inorganic Carbon:Particulate Organic Carbon Measurements of Calcifying Algae
Abstract Calcifying algae, like coccolithophores, greatly contribute to the oceanic carbon cycle and are therefore of particular interest for ocean carbon models. They play a key role in two processes that are important for the effective CO2 flux: The organic carbon pump (photosynthesis) and the ino...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-03-01
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| Series: | Global Challenges |
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| Online Access: | https://doi.org/10.1002/gch2.202200151 |
| _version_ | 1811154946851078144 |
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| author | Douwe S. deBruijn Dedmer B. Van de Waal Nico R. Helmsing Wouter Olthuis Albert van denBerg |
| author_facet | Douwe S. deBruijn Dedmer B. Van de Waal Nico R. Helmsing Wouter Olthuis Albert van denBerg |
| author_sort | Douwe S. deBruijn |
| collection | DOAJ |
| description | Abstract Calcifying algae, like coccolithophores, greatly contribute to the oceanic carbon cycle and are therefore of particular interest for ocean carbon models. They play a key role in two processes that are important for the effective CO2 flux: The organic carbon pump (photosynthesis) and the inorganic carbon pump (calcification). The relative contribution of calcification and photosynthesis can be measured in algae by the amount of particulate inorganic carbon (PIC) and particulate organic carbon (POC). A microfluidic impedance cytometer is presented, enabling non‐invasive and high‐throughput assessment of the calcification state of single coccolithophore cells. Gradual modification of the exoskeleton by acidification results in a strong linear fit (R2 = 0.98) between the average electrical phase and the PIC:POC ratio of the coccolithophore Emiliania huxleyi 920/9. The effect of different CO2 treatments on the PIC:POC ratio, however, is inconclusive, indicating that there is no strong effect observed for this particular strain. Lower PIC:POC ratios in cultures that grew to higher cell densities are found, which are also recorded with the impedance‐based PIC:POC sensor. The development of this new quantification tool for small volumes paves the way for high‐throughput analysis while applying multi‐variable environmental stressors to support projections of the future marine carbon cycle. |
| first_indexed | 2024-04-10T04:25:48Z |
| format | Article |
| id | doaj.art-1e706ffa16234ec0abe850531611b33f |
| institution | Directory Open Access Journal |
| issn | 2056-6646 |
| language | English |
| last_indexed | 2024-04-10T04:25:48Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Global Challenges |
| spelling | doaj.art-1e706ffa16234ec0abe850531611b33f2023-03-10T14:08:09ZengWileyGlobal Challenges2056-66462023-03-0173n/an/a10.1002/gch2.202200151Microfluidic Impedance Cytometry for Single‐Cell Particulate Inorganic Carbon:Particulate Organic Carbon Measurements of Calcifying AlgaeDouwe S. deBruijn0Dedmer B. Van de Waal1Nico R. Helmsing2Wouter Olthuis3Albert van denBerg4BIOS Lab‐on‐a‐Chip group MESA+ Institute for Nanotechnology Max Planck—University of Twente Center for Complex Fluid Dynamics University of Twente Drienerlolaan 5 Enschede Overijssel 7522 NB The NetherlandsDepartment of Aquatic Ecology Netherlands Institute of Ecology (NIOO‐KNAW) Droevendaalsesteeg 10 Wageningen 6708 PB The NetherlandsDepartment of Aquatic Ecology Netherlands Institute of Ecology (NIOO‐KNAW) Droevendaalsesteeg 10 Wageningen 6708 PB The NetherlandsBIOS Lab‐on‐a‐Chip group MESA+ Institute for Nanotechnology Max Planck—University of Twente Center for Complex Fluid Dynamics University of Twente Drienerlolaan 5 Enschede Overijssel 7522 NB The NetherlandsBIOS Lab‐on‐a‐Chip group MESA+ Institute for Nanotechnology Max Planck—University of Twente Center for Complex Fluid Dynamics University of Twente Drienerlolaan 5 Enschede Overijssel 7522 NB The NetherlandsAbstract Calcifying algae, like coccolithophores, greatly contribute to the oceanic carbon cycle and are therefore of particular interest for ocean carbon models. They play a key role in two processes that are important for the effective CO2 flux: The organic carbon pump (photosynthesis) and the inorganic carbon pump (calcification). The relative contribution of calcification and photosynthesis can be measured in algae by the amount of particulate inorganic carbon (PIC) and particulate organic carbon (POC). A microfluidic impedance cytometer is presented, enabling non‐invasive and high‐throughput assessment of the calcification state of single coccolithophore cells. Gradual modification of the exoskeleton by acidification results in a strong linear fit (R2 = 0.98) between the average electrical phase and the PIC:POC ratio of the coccolithophore Emiliania huxleyi 920/9. The effect of different CO2 treatments on the PIC:POC ratio, however, is inconclusive, indicating that there is no strong effect observed for this particular strain. Lower PIC:POC ratios in cultures that grew to higher cell densities are found, which are also recorded with the impedance‐based PIC:POC sensor. The development of this new quantification tool for small volumes paves the way for high‐throughput analysis while applying multi‐variable environmental stressors to support projections of the future marine carbon cycle.https://doi.org/10.1002/gch2.202200151calcifying algaemicrofluidic impedance cytometryocean carbon cyclePIC:POC ratiosingle‐cell characterization |
| spellingShingle | Douwe S. deBruijn Dedmer B. Van de Waal Nico R. Helmsing Wouter Olthuis Albert van denBerg Microfluidic Impedance Cytometry for Single‐Cell Particulate Inorganic Carbon:Particulate Organic Carbon Measurements of Calcifying Algae Global Challenges calcifying algae microfluidic impedance cytometry ocean carbon cycle PIC:POC ratio single‐cell characterization |
| title | Microfluidic Impedance Cytometry for Single‐Cell Particulate Inorganic Carbon:Particulate Organic Carbon Measurements of Calcifying Algae |
| title_full | Microfluidic Impedance Cytometry for Single‐Cell Particulate Inorganic Carbon:Particulate Organic Carbon Measurements of Calcifying Algae |
| title_fullStr | Microfluidic Impedance Cytometry for Single‐Cell Particulate Inorganic Carbon:Particulate Organic Carbon Measurements of Calcifying Algae |
| title_full_unstemmed | Microfluidic Impedance Cytometry for Single‐Cell Particulate Inorganic Carbon:Particulate Organic Carbon Measurements of Calcifying Algae |
| title_short | Microfluidic Impedance Cytometry for Single‐Cell Particulate Inorganic Carbon:Particulate Organic Carbon Measurements of Calcifying Algae |
| title_sort | microfluidic impedance cytometry for single cell particulate inorganic carbon particulate organic carbon measurements of calcifying algae |
| topic | calcifying algae microfluidic impedance cytometry ocean carbon cycle PIC:POC ratio single‐cell characterization |
| url | https://doi.org/10.1002/gch2.202200151 |
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