Pharmacological Effects of Guava (<i>Psidium guajava</i> L.) Seed Polysaccharides: GSF3 Inhibits PC-3 Prostate Cancer Cell Growth through Immunotherapy In Vitro
The inhibitory effects of purified fractions isolated from guava seed polysaccharides (GSPS) including guava seed polysaccharide fraction 1 (GSF1), GSF2, and GSF3 on prostate cancer cells remain unclear. To clarify the anti-prostate cancer potential, GSPS, GSF1, GSF2, and GSF3 were isolated using Se...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/7/3631 |
| _version_ | 1797539380262338560 |
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| author | Hsiao-Chien Lin Jin-Yuarn Lin |
| author_facet | Hsiao-Chien Lin Jin-Yuarn Lin |
| author_sort | Hsiao-Chien Lin |
| collection | DOAJ |
| description | The inhibitory effects of purified fractions isolated from guava seed polysaccharides (GSPS) including guava seed polysaccharide fraction 1 (GSF1), GSF2, and GSF3 on prostate cancer cells remain unclear. To clarify the anti-prostate cancer potential, GSPS, GSF1, GSF2, and GSF3 were isolated using Sepharose 6B gel filtration chromatography to assay their inhibitory effects on prostate PC-3 cell growth with direct action or indirect immunotherapy using either splenocyte conditioned media (SCM) or macrophage conditioned media (MCM). Correlations between cytokine profiles in the conditioned media and pro-apoptotic gene expression levels in the corresponding treated PC-3 cells were analyzed. Results showed that GSPS, GSF1, GSF2, and GSF3, particularly GSF3, through either direct action or indirect treatments using SCM or MCM, significantly (<i>p</i> < 0.05) inhibited PC-3 cell growth. GSF3 direct treatments increased pro-apoptotic Bax/anti-apoptotic Bcl-2 mRNA expression ratios in corresponding treated PC-3 cells. Either SCM or MCM cultured with GSF3 increased Fas mRNA expression levels in corresponding treated PC-3 cells. Both Th2-polarized and anti-inflammatory cytokine IL-10 either secreted in SCM or MCM were positively correlated with Fas mRNA expression levels in corresponding treated PC-3 cells. Our results suggest that GSF3 is a potent biological response modifier to decrease PC-3 cell growth through inducing apoptosis. |
| first_indexed | 2024-03-10T12:44:12Z |
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| id | doaj.art-1e718212d77f42b69d7d83e28e67f754 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:44:12Z |
| publishDate | 2021-03-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1e718212d77f42b69d7d83e28e67f7542023-11-21T13:34:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01227363110.3390/ijms22073631Pharmacological Effects of Guava (<i>Psidium guajava</i> L.) Seed Polysaccharides: GSF3 Inhibits PC-3 Prostate Cancer Cell Growth through Immunotherapy In VitroHsiao-Chien Lin0Jin-Yuarn Lin1Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227, TaiwanDepartment of Food Science and Biotechnology, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227, TaiwanThe inhibitory effects of purified fractions isolated from guava seed polysaccharides (GSPS) including guava seed polysaccharide fraction 1 (GSF1), GSF2, and GSF3 on prostate cancer cells remain unclear. To clarify the anti-prostate cancer potential, GSPS, GSF1, GSF2, and GSF3 were isolated using Sepharose 6B gel filtration chromatography to assay their inhibitory effects on prostate PC-3 cell growth with direct action or indirect immunotherapy using either splenocyte conditioned media (SCM) or macrophage conditioned media (MCM). Correlations between cytokine profiles in the conditioned media and pro-apoptotic gene expression levels in the corresponding treated PC-3 cells were analyzed. Results showed that GSPS, GSF1, GSF2, and GSF3, particularly GSF3, through either direct action or indirect treatments using SCM or MCM, significantly (<i>p</i> < 0.05) inhibited PC-3 cell growth. GSF3 direct treatments increased pro-apoptotic Bax/anti-apoptotic Bcl-2 mRNA expression ratios in corresponding treated PC-3 cells. Either SCM or MCM cultured with GSF3 increased Fas mRNA expression levels in corresponding treated PC-3 cells. Both Th2-polarized and anti-inflammatory cytokine IL-10 either secreted in SCM or MCM were positively correlated with Fas mRNA expression levels in corresponding treated PC-3 cells. Our results suggest that GSF3 is a potent biological response modifier to decrease PC-3 cell growth through inducing apoptosis.https://www.mdpi.com/1422-0067/22/7/3631<i>Fas</i> geneguava seedGSF3pro-(<i>Bax</i>)/anti-apoptotic (<i>Bcl-2</i>) genes<i>Psidium guajava</i> L.prostate cancer |
| spellingShingle | Hsiao-Chien Lin Jin-Yuarn Lin Pharmacological Effects of Guava (<i>Psidium guajava</i> L.) Seed Polysaccharides: GSF3 Inhibits PC-3 Prostate Cancer Cell Growth through Immunotherapy In Vitro International Journal of Molecular Sciences <i>Fas</i> gene guava seed GSF3 pro-(<i>Bax</i>)/anti-apoptotic (<i>Bcl-2</i>) genes <i>Psidium guajava</i> L. prostate cancer |
| title | Pharmacological Effects of Guava (<i>Psidium guajava</i> L.) Seed Polysaccharides: GSF3 Inhibits PC-3 Prostate Cancer Cell Growth through Immunotherapy In Vitro |
| title_full | Pharmacological Effects of Guava (<i>Psidium guajava</i> L.) Seed Polysaccharides: GSF3 Inhibits PC-3 Prostate Cancer Cell Growth through Immunotherapy In Vitro |
| title_fullStr | Pharmacological Effects of Guava (<i>Psidium guajava</i> L.) Seed Polysaccharides: GSF3 Inhibits PC-3 Prostate Cancer Cell Growth through Immunotherapy In Vitro |
| title_full_unstemmed | Pharmacological Effects of Guava (<i>Psidium guajava</i> L.) Seed Polysaccharides: GSF3 Inhibits PC-3 Prostate Cancer Cell Growth through Immunotherapy In Vitro |
| title_short | Pharmacological Effects of Guava (<i>Psidium guajava</i> L.) Seed Polysaccharides: GSF3 Inhibits PC-3 Prostate Cancer Cell Growth through Immunotherapy In Vitro |
| title_sort | pharmacological effects of guava i psidium guajava i l seed polysaccharides gsf3 inhibits pc 3 prostate cancer cell growth through immunotherapy in vitro |
| topic | <i>Fas</i> gene guava seed GSF3 pro-(<i>Bax</i>)/anti-apoptotic (<i>Bcl-2</i>) genes <i>Psidium guajava</i> L. prostate cancer |
| url | https://www.mdpi.com/1422-0067/22/7/3631 |
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