ANALYSIS OF POLYMORPHISM OF BETA CASEIN OF SLOVAK PINZGAU CATTLE BY PCR-RFLP FOR ALLELS A1 AND A2
The work was oriented to identification of -casein gene polymorphism and analysis of genotype structure in population of Slovak Pinzgau cattle. The material involved 89 cattle. Bovine genomic DNA was isolated by fenol-chlorophorm deprotenization and ethanol precipitation and used in order to estima...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-10-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1508 |
| _version_ | 1797215742213488640 |
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| author | MARTINA MILUCHOVÁ ANNA TRAKOVICKÁ M. GÁBOR |
| author_facet | MARTINA MILUCHOVÁ ANNA TRAKOVICKÁ M. GÁBOR |
| author_sort | MARTINA MILUCHOVÁ |
| collection | DOAJ |
| description | The work was oriented to identification of -casein gene polymorphism and
analysis of genotype structure in population of Slovak Pinzgau cattle. The material
involved 89 cattle. Bovine genomic DNA was isolated by fenol-chlorophorm
deprotenization and ethanol precipitation and used in order to estimate - casein
genotypes by means of PCR-RFLP method. The PCR products were digested with
DdeI restriction enzyme. In the population included in the study there were
homozygote genotype A1A1 (27 animals), heterozygote genotype A1A2 (46 animals)
and homozygote genotype A2A2 (16 animals). In the total population of cattle
heterozygotes A1A2 – 0.5168 were the most frequent, while homozygotes A2A2 –
0.1798 were the least frequent ones. This suggests a slight superiority of allele A1–
0.5618. |
| first_indexed | 2024-04-24T11:34:54Z |
| format | Article |
| id | doaj.art-1e721206636443aeae0e4fafecede1d6 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:34:54Z |
| publishDate | 2023-10-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-1e721206636443aeae0e4fafecede1d62024-04-10T05:28:00ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-10-014222882881508ANALYSIS OF POLYMORPHISM OF BETA CASEIN OF SLOVAK PINZGAU CATTLE BY PCR-RFLP FOR ALLELS A1 AND A2MARTINA MILUCHOVÁ0ANNA TRAKOVICKÁ1M. GÁBOR2Slovak University of Agriculture in NitraSlovak University of Agriculture in NitraSlovak University of Agriculture in NitraThe work was oriented to identification of -casein gene polymorphism and analysis of genotype structure in population of Slovak Pinzgau cattle. The material involved 89 cattle. Bovine genomic DNA was isolated by fenol-chlorophorm deprotenization and ethanol precipitation and used in order to estimate - casein genotypes by means of PCR-RFLP method. The PCR products were digested with DdeI restriction enzyme. In the population included in the study there were homozygote genotype A1A1 (27 animals), heterozygote genotype A1A2 (46 animals) and homozygote genotype A2A2 (16 animals). In the total population of cattle heterozygotes A1A2 – 0.5168 were the most frequent, while homozygotes A2A2 – 0.1798 were the least frequent ones. This suggests a slight superiority of allele A1– 0.5618.https://spasb.ro/index.php/public_html/article/view/1508cattlepcr-rflp-casein |
| spellingShingle | MARTINA MILUCHOVÁ ANNA TRAKOVICKÁ M. GÁBOR ANALYSIS OF POLYMORPHISM OF BETA CASEIN OF SLOVAK PINZGAU CATTLE BY PCR-RFLP FOR ALLELS A1 AND A2 Scientific Papers Animal Science and Biotechnologies cattle pcr-rflp -casein |
| title | ANALYSIS OF POLYMORPHISM OF BETA CASEIN OF SLOVAK PINZGAU CATTLE BY PCR-RFLP FOR ALLELS A1 AND A2 |
| title_full | ANALYSIS OF POLYMORPHISM OF BETA CASEIN OF SLOVAK PINZGAU CATTLE BY PCR-RFLP FOR ALLELS A1 AND A2 |
| title_fullStr | ANALYSIS OF POLYMORPHISM OF BETA CASEIN OF SLOVAK PINZGAU CATTLE BY PCR-RFLP FOR ALLELS A1 AND A2 |
| title_full_unstemmed | ANALYSIS OF POLYMORPHISM OF BETA CASEIN OF SLOVAK PINZGAU CATTLE BY PCR-RFLP FOR ALLELS A1 AND A2 |
| title_short | ANALYSIS OF POLYMORPHISM OF BETA CASEIN OF SLOVAK PINZGAU CATTLE BY PCR-RFLP FOR ALLELS A1 AND A2 |
| title_sort | analysis of polymorphism of beta casein of slovak pinzgau cattle by pcr rflp for allels a1 and a2 |
| topic | cattle pcr-rflp -casein |
| url | https://spasb.ro/index.php/public_html/article/view/1508 |
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