Simple diagnosis of cancer by detecting CEA and CYFRA 21-1 in saliva using electronic sensors
Abstract One way of early diagnosis of cancer is by detecting the biomarkers that get introduced into easily accessible body fluids. We report the development of portable and rapid electronic biosensors for quantitative detection of two secretive cancer biomarkers–Carcinoembryonic antigen (CEA) and...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-19593-8 |
| _version_ | 1828105324553306112 |
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| author | Sowmya Joshi Shashidhar Kallappa Pranjal Kumar Sudhanshu Shukla Ruma Ghosh |
| author_facet | Sowmya Joshi Shashidhar Kallappa Pranjal Kumar Sudhanshu Shukla Ruma Ghosh |
| author_sort | Sowmya Joshi |
| collection | DOAJ |
| description | Abstract One way of early diagnosis of cancer is by detecting the biomarkers that get introduced into easily accessible body fluids. We report the development of portable and rapid electronic biosensors for quantitative detection of two secretive cancer biomarkers–Carcinoembryonic antigen (CEA) and Cytokeratin fragment 19 (CYFRA 21-1). The reduced graphene oxide (rGO)/ melamine (MEL)/antibodies/ bovine serum albumin (BSA) based devices were tested for 1 pg/mL to 800 ng/mL of CEA and CYFRA 21-1. The responses of the sensors ranged from 7.14 to 59.1% and from 6.18 to 64% for 1 pg/mL to 800 ng/mL CEA and CYFRA 21-1 respectively. A read-out circuit was assembled to develop a portable prototype which was used to assess the concentrations of the two antigens present in saliva samples of 14 subjects. The prototype could accurately discriminate between 9 oral squamous cell carcinoma patients and 5 healthy controls. |
| first_indexed | 2024-04-11T10:01:27Z |
| format | Article |
| id | doaj.art-1e7532fcf8d84642a976394b093179a4 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T10:01:27Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1e7532fcf8d84642a976394b093179a42022-12-22T04:30:24ZengNature PortfolioScientific Reports2045-23222022-09-0112111310.1038/s41598-022-19593-8Simple diagnosis of cancer by detecting CEA and CYFRA 21-1 in saliva using electronic sensorsSowmya Joshi0Shashidhar Kallappa1Pranjal Kumar2Sudhanshu Shukla3Ruma Ghosh4Department of Electrical Engineering, Indian Institute of Technology DharwadDepartment of Surgical Oncology, Karnataka Institute of Medical SciencesDepartment of Biosciences and Bioengineering, Indian Institute of Technology DharwadDepartment of Biosciences and Bioengineering, Indian Institute of Technology DharwadDepartment of Electrical Engineering, Indian Institute of Technology DharwadAbstract One way of early diagnosis of cancer is by detecting the biomarkers that get introduced into easily accessible body fluids. We report the development of portable and rapid electronic biosensors for quantitative detection of two secretive cancer biomarkers–Carcinoembryonic antigen (CEA) and Cytokeratin fragment 19 (CYFRA 21-1). The reduced graphene oxide (rGO)/ melamine (MEL)/antibodies/ bovine serum albumin (BSA) based devices were tested for 1 pg/mL to 800 ng/mL of CEA and CYFRA 21-1. The responses of the sensors ranged from 7.14 to 59.1% and from 6.18 to 64% for 1 pg/mL to 800 ng/mL CEA and CYFRA 21-1 respectively. A read-out circuit was assembled to develop a portable prototype which was used to assess the concentrations of the two antigens present in saliva samples of 14 subjects. The prototype could accurately discriminate between 9 oral squamous cell carcinoma patients and 5 healthy controls.https://doi.org/10.1038/s41598-022-19593-8 |
| spellingShingle | Sowmya Joshi Shashidhar Kallappa Pranjal Kumar Sudhanshu Shukla Ruma Ghosh Simple diagnosis of cancer by detecting CEA and CYFRA 21-1 in saliva using electronic sensors Scientific Reports |
| title | Simple diagnosis of cancer by detecting CEA and CYFRA 21-1 in saliva using electronic sensors |
| title_full | Simple diagnosis of cancer by detecting CEA and CYFRA 21-1 in saliva using electronic sensors |
| title_fullStr | Simple diagnosis of cancer by detecting CEA and CYFRA 21-1 in saliva using electronic sensors |
| title_full_unstemmed | Simple diagnosis of cancer by detecting CEA and CYFRA 21-1 in saliva using electronic sensors |
| title_short | Simple diagnosis of cancer by detecting CEA and CYFRA 21-1 in saliva using electronic sensors |
| title_sort | simple diagnosis of cancer by detecting cea and cyfra 21 1 in saliva using electronic sensors |
| url | https://doi.org/10.1038/s41598-022-19593-8 |
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