Melatonin Application Modifies Antioxidant Defense and Induces Endoreplication in Maize Seeds Exposed to Chilling Stress
The aim of the study was to demonstrate the biostimulating effect of exogenous melatonin (MEL) applied to seeds via hydroconditioning. It was indicated that only well-chosen application technique and MEL dose guarantees success concerning seed germination and young seedlings growth under stress cond...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/16/8628 |
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| author | Izabela Kołodziejczyk Andrzej Kaźmierczak Małgorzata M. Posmyk |
| author_facet | Izabela Kołodziejczyk Andrzej Kaźmierczak Małgorzata M. Posmyk |
| author_sort | Izabela Kołodziejczyk |
| collection | DOAJ |
| description | The aim of the study was to demonstrate the biostimulating effect of exogenous melatonin (MEL) applied to seeds via hydroconditioning. It was indicated that only well-chosen application technique and MEL dose guarantees success concerning seed germination and young seedlings growth under stress conditions. For maize seed, 50 μM of MEL appeared to be the optimal dose. It improved seed germination and embryonic axes growth especially during chilling stress (5 °C/14 days) and during regeneration after its subsided. Unfortunately, MEL overdosing lowered IAA level in dry seeds and could disrupt the ROS-dependent signal transduction pathways. Very effective antioxidant MEL action was confirmed by low level of protein oxidative damage and smaller quantity of lipid oxidation products in embryonic axes isolated from seeds pre-treated with MEL and then exposed to cold. The stimulatory effects of MEL on antioxidant enzymes: SOD, APX and GSH-PX and on GST-a detoxifying enzyme, was also demonstrated. It was indicated for the first time, that MEL induced defence strategies against stress at the cytological level, as appearing endoreplication in embryonic axes cells even in the seeds germinating under optimal conditions (preventive action), but very intensively in those germinating under chilling stress conditions (intervention action), and after stress removal, to improve regeneration. |
| first_indexed | 2024-03-10T08:44:34Z |
| format | Article |
| id | doaj.art-1e76558f41c74de2afa5c7db5fd116fc |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:44:34Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1e76558f41c74de2afa5c7db5fd116fc2023-11-22T07:58:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216862810.3390/ijms22168628Melatonin Application Modifies Antioxidant Defense and Induces Endoreplication in Maize Seeds Exposed to Chilling StressIzabela Kołodziejczyk0Andrzej Kaźmierczak1Małgorzata M. Posmyk2Department of Plant Ecophisiology, Faculty of Biology and Environmental Protection, University of Lodz, 90237 Lodz, PolandDepartment of Cytophysiology, Faculty of Biology and Environmental Protection, University of Lodz, 90237 Lodz, PolandDepartment of Plant Ecophisiology, Faculty of Biology and Environmental Protection, University of Lodz, 90237 Lodz, PolandThe aim of the study was to demonstrate the biostimulating effect of exogenous melatonin (MEL) applied to seeds via hydroconditioning. It was indicated that only well-chosen application technique and MEL dose guarantees success concerning seed germination and young seedlings growth under stress conditions. For maize seed, 50 μM of MEL appeared to be the optimal dose. It improved seed germination and embryonic axes growth especially during chilling stress (5 °C/14 days) and during regeneration after its subsided. Unfortunately, MEL overdosing lowered IAA level in dry seeds and could disrupt the ROS-dependent signal transduction pathways. Very effective antioxidant MEL action was confirmed by low level of protein oxidative damage and smaller quantity of lipid oxidation products in embryonic axes isolated from seeds pre-treated with MEL and then exposed to cold. The stimulatory effects of MEL on antioxidant enzymes: SOD, APX and GSH-PX and on GST-a detoxifying enzyme, was also demonstrated. It was indicated for the first time, that MEL induced defence strategies against stress at the cytological level, as appearing endoreplication in embryonic axes cells even in the seeds germinating under optimal conditions (preventive action), but very intensively in those germinating under chilling stress conditions (intervention action), and after stress removal, to improve regeneration.https://www.mdpi.com/1422-0067/22/16/8628antioxidant enzymesbiostimulationchilling stresscorn seedsendoreplicationmelatonin |
| spellingShingle | Izabela Kołodziejczyk Andrzej Kaźmierczak Małgorzata M. Posmyk Melatonin Application Modifies Antioxidant Defense and Induces Endoreplication in Maize Seeds Exposed to Chilling Stress International Journal of Molecular Sciences antioxidant enzymes biostimulation chilling stress corn seeds endoreplication melatonin |
| title | Melatonin Application Modifies Antioxidant Defense and Induces Endoreplication in Maize Seeds Exposed to Chilling Stress |
| title_full | Melatonin Application Modifies Antioxidant Defense and Induces Endoreplication in Maize Seeds Exposed to Chilling Stress |
| title_fullStr | Melatonin Application Modifies Antioxidant Defense and Induces Endoreplication in Maize Seeds Exposed to Chilling Stress |
| title_full_unstemmed | Melatonin Application Modifies Antioxidant Defense and Induces Endoreplication in Maize Seeds Exposed to Chilling Stress |
| title_short | Melatonin Application Modifies Antioxidant Defense and Induces Endoreplication in Maize Seeds Exposed to Chilling Stress |
| title_sort | melatonin application modifies antioxidant defense and induces endoreplication in maize seeds exposed to chilling stress |
| topic | antioxidant enzymes biostimulation chilling stress corn seeds endoreplication melatonin |
| url | https://www.mdpi.com/1422-0067/22/16/8628 |
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