Xenosurveillance reflects traditional sampling techniques for the identification of human pathogens: A comparative study in West Africa.
Novel surveillance strategies are needed to detect the rapid and continuous emergence of infectious disease agents. Ideally, new sampling strategies should be simple to implement, technologically uncomplicated, and applicable to areas where emergence events are known to occur. To this end, xenosurve...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-03-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | http://europepmc.org/articles/PMC5880402?pdf=render |
| _version_ | 1818517476777918464 |
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| author | Joseph R Fauver James Weger-Lucarelli Lawrence S Fakoli Kpehe Bolay Fatorma K Bolay Joseph W Diclaro Doug E Brackney Brian D Foy Mark D Stenglein Gregory D Ebel |
| author_facet | Joseph R Fauver James Weger-Lucarelli Lawrence S Fakoli Kpehe Bolay Fatorma K Bolay Joseph W Diclaro Doug E Brackney Brian D Foy Mark D Stenglein Gregory D Ebel |
| author_sort | Joseph R Fauver |
| collection | DOAJ |
| description | Novel surveillance strategies are needed to detect the rapid and continuous emergence of infectious disease agents. Ideally, new sampling strategies should be simple to implement, technologically uncomplicated, and applicable to areas where emergence events are known to occur. To this end, xenosurveillance is a technique that makes use of blood collected by hematophagous arthropods to monitor and identify vertebrate pathogens. Mosquitoes are largely ubiquitous animals that often exist in sizable populations. As well, many domestic or peridomestic species of mosquitoes will preferentially take blood-meals from humans, making them a unique and largely untapped reservoir to collect human blood.We sought to take advantage of this phenomenon by systematically collecting blood-fed mosquitoes during a field trail in Northern Liberia to determine whether pathogen sequences from blood engorged mosquitoes accurately mirror those obtained directly from humans. Specifically, blood was collected from humans via finger-stick and by aspirating bloodfed mosquitoes from the inside of houses. Shotgun metagenomic sequencing of RNA and DNA derived from these specimens was performed to detect pathogen sequences. Samples obtained from xenosurveillance and from finger-stick blood collection produced a similar number and quality of reads aligning to two human viruses, GB virus C and hepatitis B virus.This study represents the first systematic comparison between xenosurveillance and more traditional sampling methodologies, while also demonstrating the viability of xenosurveillance as a tool to sample human blood for circulating pathogens. |
| first_indexed | 2024-12-11T00:56:47Z |
| format | Article |
| id | doaj.art-1e776fe70de54d1b9d10410520f689d5 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-11T00:56:47Z |
| publishDate | 2018-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-1e776fe70de54d1b9d10410520f689d52022-12-22T01:26:26ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352018-03-01123e000634810.1371/journal.pntd.0006348Xenosurveillance reflects traditional sampling techniques for the identification of human pathogens: A comparative study in West Africa.Joseph R FauverJames Weger-LucarelliLawrence S FakoliKpehe BolayFatorma K BolayJoseph W DiclaroDoug E BrackneyBrian D FoyMark D StengleinGregory D EbelNovel surveillance strategies are needed to detect the rapid and continuous emergence of infectious disease agents. Ideally, new sampling strategies should be simple to implement, technologically uncomplicated, and applicable to areas where emergence events are known to occur. To this end, xenosurveillance is a technique that makes use of blood collected by hematophagous arthropods to monitor and identify vertebrate pathogens. Mosquitoes are largely ubiquitous animals that often exist in sizable populations. As well, many domestic or peridomestic species of mosquitoes will preferentially take blood-meals from humans, making them a unique and largely untapped reservoir to collect human blood.We sought to take advantage of this phenomenon by systematically collecting blood-fed mosquitoes during a field trail in Northern Liberia to determine whether pathogen sequences from blood engorged mosquitoes accurately mirror those obtained directly from humans. Specifically, blood was collected from humans via finger-stick and by aspirating bloodfed mosquitoes from the inside of houses. Shotgun metagenomic sequencing of RNA and DNA derived from these specimens was performed to detect pathogen sequences. Samples obtained from xenosurveillance and from finger-stick blood collection produced a similar number and quality of reads aligning to two human viruses, GB virus C and hepatitis B virus.This study represents the first systematic comparison between xenosurveillance and more traditional sampling methodologies, while also demonstrating the viability of xenosurveillance as a tool to sample human blood for circulating pathogens.http://europepmc.org/articles/PMC5880402?pdf=render |
| spellingShingle | Joseph R Fauver James Weger-Lucarelli Lawrence S Fakoli Kpehe Bolay Fatorma K Bolay Joseph W Diclaro Doug E Brackney Brian D Foy Mark D Stenglein Gregory D Ebel Xenosurveillance reflects traditional sampling techniques for the identification of human pathogens: A comparative study in West Africa. PLoS Neglected Tropical Diseases |
| title | Xenosurveillance reflects traditional sampling techniques for the identification of human pathogens: A comparative study in West Africa. |
| title_full | Xenosurveillance reflects traditional sampling techniques for the identification of human pathogens: A comparative study in West Africa. |
| title_fullStr | Xenosurveillance reflects traditional sampling techniques for the identification of human pathogens: A comparative study in West Africa. |
| title_full_unstemmed | Xenosurveillance reflects traditional sampling techniques for the identification of human pathogens: A comparative study in West Africa. |
| title_short | Xenosurveillance reflects traditional sampling techniques for the identification of human pathogens: A comparative study in West Africa. |
| title_sort | xenosurveillance reflects traditional sampling techniques for the identification of human pathogens a comparative study in west africa |
| url | http://europepmc.org/articles/PMC5880402?pdf=render |
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