Characterization of Dextran Produced by the Food-Related Strain <i>Weissella cibaria</i> C43-11 and of the Relevant <i>Dextransucrase</i> Gene
A metabolic feature of lactic acid bacteria (LAB) is the production of exopolysaccharides (EPSs), which have technological and functional properties of interest to the food sector. The present study focused on the characterization of the <i>Weissella cibaria</i> strain C43-11, a high EPS...
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MDPI AG
2022-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/18/2819 |
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| author | Palmira De Bellis Massimo Ferrara Anna Rita Bavaro Vito Linsalata Mariaelena Di Biase Biagia Musio Vito Gallo Giuseppina Mulè Francesca Valerio |
| author_facet | Palmira De Bellis Massimo Ferrara Anna Rita Bavaro Vito Linsalata Mariaelena Di Biase Biagia Musio Vito Gallo Giuseppina Mulè Francesca Valerio |
| author_sort | Palmira De Bellis |
| collection | DOAJ |
| description | A metabolic feature of lactic acid bacteria (LAB) is the production of exopolysaccharides (EPSs), which have technological and functional properties of interest to the food sector. The present study focused on the characterization of the <i>Weissella cibaria</i> strain C43-11, a high EPS producer in the presence of sucrose, in comparison with a low-producing strain (C2-32), and on possible genetic regulatory elements responsible for the modulation of <i>dextransucrase</i> (<i>dsr</i>) genes expression. NMR analysis of the polymeric material produced by the C43-11 strain indicated the presence of dextran consisting mainly of a linear scaffold formed by α-(1–6) glycosidic linkages and a smaller amounts of branches derived from α-(1–2), α-(1–3), and α-(1–4) linkages. Molecular analysis of the <i>dsr</i> genes and the putative transcriptional promoters of the two strains showed differences in their regulatory regions. Such variations may have a role in the modulation of <i>dsr</i> expression levels in the presence of sucrose. The strong upregulation of the <i>dsr</i> gene in the C43-11 strain resulted in a high accumulation of EPS. This is the first report showing differences in the regulatory elements of the <i>dsr</i> gene in <i>W. cibaria</i> and indicates a new perspective of investigation to identify the regulatory mechanism of EPS production. |
| first_indexed | 2024-03-10T00:02:03Z |
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| id | doaj.art-1e7b9e9531e84d50880491ae8de652ff |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T00:02:03Z |
| publishDate | 2022-09-01 |
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| record_format | Article |
| series | Foods |
| spelling | doaj.art-1e7b9e9531e84d50880491ae8de652ff2023-11-23T16:13:42ZengMDPI AGFoods2304-81582022-09-011118281910.3390/foods11182819Characterization of Dextran Produced by the Food-Related Strain <i>Weissella cibaria</i> C43-11 and of the Relevant <i>Dextransucrase</i> GenePalmira De Bellis0Massimo Ferrara1Anna Rita Bavaro2Vito Linsalata3Mariaelena Di Biase4Biagia Musio5Vito Gallo6Giuseppina Mulè7Francesca Valerio8Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, ItalyInstitute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, ItalyInstitute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, ItalyInstitute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, ItalyInstitute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, ItalyDepartment of Civil, Environmental, Land, Building and Chemical Engineering, Dicatech, Polytechnic University of Bari, Via Edoardo Orabona 4, 70125 Bari, ItalyDepartment of Civil, Environmental, Land, Building and Chemical Engineering, Dicatech, Polytechnic University of Bari, Via Edoardo Orabona 4, 70125 Bari, ItalyInstitute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, ItalyInstitute of Sciences of Food Production (ISPA), National Research Council (CNR), Via G. Amendola 122/O, 70126 Bari, ItalyA metabolic feature of lactic acid bacteria (LAB) is the production of exopolysaccharides (EPSs), which have technological and functional properties of interest to the food sector. The present study focused on the characterization of the <i>Weissella cibaria</i> strain C43-11, a high EPS producer in the presence of sucrose, in comparison with a low-producing strain (C2-32), and on possible genetic regulatory elements responsible for the modulation of <i>dextransucrase</i> (<i>dsr</i>) genes expression. NMR analysis of the polymeric material produced by the C43-11 strain indicated the presence of dextran consisting mainly of a linear scaffold formed by α-(1–6) glycosidic linkages and a smaller amounts of branches derived from α-(1–2), α-(1–3), and α-(1–4) linkages. Molecular analysis of the <i>dsr</i> genes and the putative transcriptional promoters of the two strains showed differences in their regulatory regions. Such variations may have a role in the modulation of <i>dsr</i> expression levels in the presence of sucrose. The strong upregulation of the <i>dsr</i> gene in the C43-11 strain resulted in a high accumulation of EPS. This is the first report showing differences in the regulatory elements of the <i>dsr</i> gene in <i>W. cibaria</i> and indicates a new perspective of investigation to identify the regulatory mechanism of EPS production.https://www.mdpi.com/2304-8158/11/18/2819lactic acid bacteriaexopolysaccharideshomopolysaccharidesdextransucrosegene expression |
| spellingShingle | Palmira De Bellis Massimo Ferrara Anna Rita Bavaro Vito Linsalata Mariaelena Di Biase Biagia Musio Vito Gallo Giuseppina Mulè Francesca Valerio Characterization of Dextran Produced by the Food-Related Strain <i>Weissella cibaria</i> C43-11 and of the Relevant <i>Dextransucrase</i> Gene Foods lactic acid bacteria exopolysaccharides homopolysaccharides dextran sucrose gene expression |
| title | Characterization of Dextran Produced by the Food-Related Strain <i>Weissella cibaria</i> C43-11 and of the Relevant <i>Dextransucrase</i> Gene |
| title_full | Characterization of Dextran Produced by the Food-Related Strain <i>Weissella cibaria</i> C43-11 and of the Relevant <i>Dextransucrase</i> Gene |
| title_fullStr | Characterization of Dextran Produced by the Food-Related Strain <i>Weissella cibaria</i> C43-11 and of the Relevant <i>Dextransucrase</i> Gene |
| title_full_unstemmed | Characterization of Dextran Produced by the Food-Related Strain <i>Weissella cibaria</i> C43-11 and of the Relevant <i>Dextransucrase</i> Gene |
| title_short | Characterization of Dextran Produced by the Food-Related Strain <i>Weissella cibaria</i> C43-11 and of the Relevant <i>Dextransucrase</i> Gene |
| title_sort | characterization of dextran produced by the food related strain i weissella cibaria i c43 11 and of the relevant i dextransucrase i gene |
| topic | lactic acid bacteria exopolysaccharides homopolysaccharides dextran sucrose gene expression |
| url | https://www.mdpi.com/2304-8158/11/18/2819 |
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