ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum
Abstract Background Zinc oxide nanoparticles (ZnONPs) have been widely studied as bactericidal reagents. However, it is still challenging to use ZnONPs as a root canal sealant to eliminate infecting microorganisms in the root canal system. This study aimed at understanding the antibacterial and biof...
| প্রধান লেখক: | , , , , |
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| বিন্যাস: | প্রবন্ধ |
| ভাষা: | English |
| প্রকাশিত: |
BMC
2019-05-01
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| মালা: | BMC Oral Health |
| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | http://link.springer.com/article/10.1186/s12903-019-0780-y |
| _version_ | 1828794723474604032 |
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| author | Jingyu Wang Lele Du Yingmei Fu Peidong Jiang Xiumei Wang |
| author_facet | Jingyu Wang Lele Du Yingmei Fu Peidong Jiang Xiumei Wang |
| author_sort | Jingyu Wang |
| collection | DOAJ |
| description | Abstract Background Zinc oxide nanoparticles (ZnONPs) have been widely studied as bactericidal reagents. However, it is still challenging to use ZnONPs as a root canal sealant to eliminate infecting microorganisms in the root canal system. This study aimed at understanding the antibacterial and biofilm effects of ZnONPs in the infected root canal and their effect on cell function. Methods This study aimed to develop a better understanding of the antibacterial effects of ZnONPs in the infected root canal and their effect on cell function. Experiments were performed in two stages; the first stage included inhibition zone tests and the minimum inhibitory concentration (MIC) test, which were performed to examine the antibacterial activity of ZnONPs against Porphyromonas gingivalis (P. gingivalis) and Actinomyces Naeslundii (A. naeslundii) bacteria in vitro. ZnONPs were further evaluated for their biocompatibility using normal mouse NIH3T3 and OCCM-30 cells by the cell-based MTT assay. In addition, the influence of ZnONPs on matrix metalloproteinases in NIH3T3 cells and their inhibiting factors (Mmp13 and Timp1) were measured using the real-time PCR technique and western blot method. Results The MIC of ZnONPs against P. gingivalis and A. naeslundii were confirmed to be 10 μg/mL and 40 μg/mL, respectively. The MTT assay showed that ZnONPs were nontoxic. The RT-PCR and western blotting results showed that Mmp13 was downregulated and Timp1 expression was increased. Meanwhile, ZnONPs were shown to increase the expression of the OCCM-30 osteogenesis-related factors Bsp and Runx2. Finally, there was no significant change in the morphology of NIH3T3 and OCCM-30 cells after the addition of different concentrations of ZnONPs for different periods of time. Conclusion ZnONPs have excellent antibacterial activity against P. gingivalis and A. naeslundii and have low cell cytotoxicity in vitro. |
| first_indexed | 2024-12-12T03:51:08Z |
| format | Article |
| id | doaj.art-1e7dd4a873a841048c304c18141a488c |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-12-12T03:51:08Z |
| publishDate | 2019-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-1e7dd4a873a841048c304c18141a488c2022-12-22T00:39:23ZengBMCBMC Oral Health1472-68312019-05-0119111110.1186/s12903-019-0780-yZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementumJingyu Wang0Lele Du1Yingmei Fu2Peidong Jiang3Xiumei Wang4Department of Dentistry, the Second Affiliated Hospital of Harbin Medical UniversityDepartment of Dentistry, the Second Affiliated Hospital of Harbin Medical UniversityWu Lien-Teh Institute, Department of Microbiology, Harbin Medical University, The Heilongjiang Key laboratory of immunity and infection, Pathogen BiologyWu Lien-Teh Institute, Department of Microbiology, Harbin Medical University, The Heilongjiang Key laboratory of immunity and infection, Pathogen BiologyDepartment of Dentistry, the Second Affiliated Hospital of Harbin Medical UniversityAbstract Background Zinc oxide nanoparticles (ZnONPs) have been widely studied as bactericidal reagents. However, it is still challenging to use ZnONPs as a root canal sealant to eliminate infecting microorganisms in the root canal system. This study aimed at understanding the antibacterial and biofilm effects of ZnONPs in the infected root canal and their effect on cell function. Methods This study aimed to develop a better understanding of the antibacterial effects of ZnONPs in the infected root canal and their effect on cell function. Experiments were performed in two stages; the first stage included inhibition zone tests and the minimum inhibitory concentration (MIC) test, which were performed to examine the antibacterial activity of ZnONPs against Porphyromonas gingivalis (P. gingivalis) and Actinomyces Naeslundii (A. naeslundii) bacteria in vitro. ZnONPs were further evaluated for their biocompatibility using normal mouse NIH3T3 and OCCM-30 cells by the cell-based MTT assay. In addition, the influence of ZnONPs on matrix metalloproteinases in NIH3T3 cells and their inhibiting factors (Mmp13 and Timp1) were measured using the real-time PCR technique and western blot method. Results The MIC of ZnONPs against P. gingivalis and A. naeslundii were confirmed to be 10 μg/mL and 40 μg/mL, respectively. The MTT assay showed that ZnONPs were nontoxic. The RT-PCR and western blotting results showed that Mmp13 was downregulated and Timp1 expression was increased. Meanwhile, ZnONPs were shown to increase the expression of the OCCM-30 osteogenesis-related factors Bsp and Runx2. Finally, there was no significant change in the morphology of NIH3T3 and OCCM-30 cells after the addition of different concentrations of ZnONPs for different periods of time. Conclusion ZnONPs have excellent antibacterial activity against P. gingivalis and A. naeslundii and have low cell cytotoxicity in vitro.http://link.springer.com/article/10.1186/s12903-019-0780-yZinc oxide nanoparticlesAntimicrobialApical periodontitisDental materialCytotoxicity |
| spellingShingle | Jingyu Wang Lele Du Yingmei Fu Peidong Jiang Xiumei Wang ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum BMC Oral Health Zinc oxide nanoparticles Antimicrobial Apical periodontitis Dental material Cytotoxicity |
| title | ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum |
| title_full | ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum |
| title_fullStr | ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum |
| title_full_unstemmed | ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum |
| title_short | ZnO nanoparticles inhibit the activity of Porphyromonas gingivalis and Actinomyces naeslundii and promote the mineralization of the cementum |
| title_sort | zno nanoparticles inhibit the activity of porphyromonas gingivalis and actinomyces naeslundii and promote the mineralization of the cementum |
| topic | Zinc oxide nanoparticles Antimicrobial Apical periodontitis Dental material Cytotoxicity |
| url | http://link.springer.com/article/10.1186/s12903-019-0780-y |
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