Presynaptic protein synthesis required for NT-3-induced long-term synaptic modulation
<p>Abstract</p> <p>Background</p> <p>Neurotrophins elicit both acute and long-term modulation of synaptic transmission and plasticity. Previously, we demonstrated that the long-term synaptic modulation requires the endocytosis of neurotrophin-receptor complex, the activ...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-01-01
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| Series: | Molecular Brain |
| Online Access: | http://www.molecularbrain.com/content/4/1/1 |
| _version_ | 1811277614645510144 |
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| author | Je H Ji Yuanyuan Wang Ying Yang Feng Wu Wei Lu Bai |
| author_facet | Je H Ji Yuanyuan Wang Ying Yang Feng Wu Wei Lu Bai |
| author_sort | Je H |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Neurotrophins elicit both acute and long-term modulation of synaptic transmission and plasticity. Previously, we demonstrated that the long-term synaptic modulation requires the endocytosis of neurotrophin-receptor complex, the activation of PI3K and Akt, and mTOR mediated protein synthesis. However, it is unclear whether the long-term synaptic modulation by neurotrophins depends on protein synthesis in pre- or post-synaptic cells.</p> <p>Results</p> <p>Here we have developed an inducible protein translation blocker, in which the kinase domain of protein kinase R (PKR) is fused with bacterial gyrase B domain (GyrB-PKR), which could be dimerized upon treatment with a cell permeable drug, coumermycin. By genetically targeting GyrB-PKR to specific cell types, we show that NT-3 induced long-term synaptic modulation requires presynaptic, but not postsynaptic protein synthesis.</p> <p>Conclusions</p> <p>Our results provide mechanistic insights into the cell-specific requirement for protein synthesis in the long-term synaptic modulation by neurotrophins. The GyrB-PKR system may be useful tool to study protein synthesis in a cell-specific manner.</p> |
| first_indexed | 2024-04-13T00:19:55Z |
| format | Article |
| id | doaj.art-1e7e94b0193b44c1b9dd68d46792e519 |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-04-13T00:19:55Z |
| publishDate | 2011-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-1e7e94b0193b44c1b9dd68d46792e5192022-12-22T03:10:49ZengBMCMolecular Brain1756-66062011-01-0141110.1186/1756-6606-4-1Presynaptic protein synthesis required for NT-3-induced long-term synaptic modulationJe HJi YuanyuanWang YingYang FengWu WeiLu Bai<p>Abstract</p> <p>Background</p> <p>Neurotrophins elicit both acute and long-term modulation of synaptic transmission and plasticity. Previously, we demonstrated that the long-term synaptic modulation requires the endocytosis of neurotrophin-receptor complex, the activation of PI3K and Akt, and mTOR mediated protein synthesis. However, it is unclear whether the long-term synaptic modulation by neurotrophins depends on protein synthesis in pre- or post-synaptic cells.</p> <p>Results</p> <p>Here we have developed an inducible protein translation blocker, in which the kinase domain of protein kinase R (PKR) is fused with bacterial gyrase B domain (GyrB-PKR), which could be dimerized upon treatment with a cell permeable drug, coumermycin. By genetically targeting GyrB-PKR to specific cell types, we show that NT-3 induced long-term synaptic modulation requires presynaptic, but not postsynaptic protein synthesis.</p> <p>Conclusions</p> <p>Our results provide mechanistic insights into the cell-specific requirement for protein synthesis in the long-term synaptic modulation by neurotrophins. The GyrB-PKR system may be useful tool to study protein synthesis in a cell-specific manner.</p>http://www.molecularbrain.com/content/4/1/1 |
| spellingShingle | Je H Ji Yuanyuan Wang Ying Yang Feng Wu Wei Lu Bai Presynaptic protein synthesis required for NT-3-induced long-term synaptic modulation Molecular Brain |
| title | Presynaptic protein synthesis required for NT-3-induced long-term synaptic modulation |
| title_full | Presynaptic protein synthesis required for NT-3-induced long-term synaptic modulation |
| title_fullStr | Presynaptic protein synthesis required for NT-3-induced long-term synaptic modulation |
| title_full_unstemmed | Presynaptic protein synthesis required for NT-3-induced long-term synaptic modulation |
| title_short | Presynaptic protein synthesis required for NT-3-induced long-term synaptic modulation |
| title_sort | presynaptic protein synthesis required for nt 3 induced long term synaptic modulation |
| url | http://www.molecularbrain.com/content/4/1/1 |
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