Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells
Jong Hwa Jun,1 Wern-Joo Sohn,2 Youngkyun Lee,2 Jae-Young Kim21Department of Ophthalmology, School of Medicine, Dongsan Medical Center, Keimyung University, 2Department of Oral Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, South KoreaAbstract: The molecular a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2016-06-01
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| Series: | Clinical Ophthalmology |
| Subjects: | |
| Online Access: | https://www.dovepress.com/effects-of-anti-vascular-endothelial-growth-factor-monoclonal-antibody-peer-reviewed-article-OPTH |
| _version_ | 1818421129860087808 |
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| author | Jun JH Sohn WJ Lee Y Kim JY |
| author_facet | Jun JH Sohn WJ Lee Y Kim JY |
| author_sort | Jun JH |
| collection | DOAJ |
| description | Jong Hwa Jun,1 Wern-Joo Sohn,2 Youngkyun Lee,2 Jae-Young Kim21Department of Ophthalmology, School of Medicine, Dongsan Medical Center, Keimyung University, 2Department of Oral Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, South KoreaAbstract: The molecular and cellular effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells (LECs) were examined using both an immortalized human lens epithelial cell line and a porcine capsular bag model. After treatment with various concentrations of bevacizumab, cell viability and proliferation patterns were evaluated using the water-soluble tetrazolium salt assay and 5-bromo-2'-deoxyuridine enzyme-linked immunosorbent assay, respectively. The scratch assay and Western blot analysis were employed to validate the cell migration pattern and altered expression levels of signaling molecules related to the epithelial–mesenchymal transition (EMT). Application of bevacizumab induced a range of altered cellular events in a concentration-dependent manner. A 0.1–2 mg/mL concentration demonstrated dose-dependent increase in proliferation and viability of LECs. However, 4 mg/mL decreased cell proliferation and viability. Cell migrations displayed dose-dependent retardation from 0.1 mg/mL bevacizumab treatment. Transforming growth factor-β2 expression was markedly increased in a dose-dependent manner, and α-smooth muscle actin, matrix metalloproteinase-9, and vimentin expression levels showed dose-dependent changes in a B3 cell line. Microscopic observation of porcine capsular bag revealed changes in cellular morphology and a decline in cell density compared to the control after 2 mg/mL treatment. The central aspect of posterior capsule showed delayed confluence, and the factors related to EMT revealed similar expression patterns to those identified in the cell line. Based on these results, bevacizumab modulates the proliferation and viability of LECs and induces morphological alterations through the modulation of expression patterns of specific factors related to the EMT.Keywords: avastin, bevacizumab, lens epithelial cell, transforming growth factor, vascular endothelial growth factor |
| first_indexed | 2024-12-14T13:05:27Z |
| format | Article |
| id | doaj.art-1e7ef79f18d54570841eaea4dcb062fb |
| institution | Directory Open Access Journal |
| issn | 1177-5483 |
| language | English |
| last_indexed | 2024-12-14T13:05:27Z |
| publishDate | 2016-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Clinical Ophthalmology |
| spelling | doaj.art-1e7ef79f18d54570841eaea4dcb062fb2022-12-21T23:00:19ZengDove Medical PressClinical Ophthalmology1177-54832016-06-012016Issue 11167117427636Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cellsJun JHSohn WJLee YKim JYJong Hwa Jun,1 Wern-Joo Sohn,2 Youngkyun Lee,2 Jae-Young Kim21Department of Ophthalmology, School of Medicine, Dongsan Medical Center, Keimyung University, 2Department of Oral Biochemistry, School of Dentistry, IHBR, Kyungpook National University, Daegu, South KoreaAbstract: The molecular and cellular effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells (LECs) were examined using both an immortalized human lens epithelial cell line and a porcine capsular bag model. After treatment with various concentrations of bevacizumab, cell viability and proliferation patterns were evaluated using the water-soluble tetrazolium salt assay and 5-bromo-2'-deoxyuridine enzyme-linked immunosorbent assay, respectively. The scratch assay and Western blot analysis were employed to validate the cell migration pattern and altered expression levels of signaling molecules related to the epithelial–mesenchymal transition (EMT). Application of bevacizumab induced a range of altered cellular events in a concentration-dependent manner. A 0.1–2 mg/mL concentration demonstrated dose-dependent increase in proliferation and viability of LECs. However, 4 mg/mL decreased cell proliferation and viability. Cell migrations displayed dose-dependent retardation from 0.1 mg/mL bevacizumab treatment. Transforming growth factor-β2 expression was markedly increased in a dose-dependent manner, and α-smooth muscle actin, matrix metalloproteinase-9, and vimentin expression levels showed dose-dependent changes in a B3 cell line. Microscopic observation of porcine capsular bag revealed changes in cellular morphology and a decline in cell density compared to the control after 2 mg/mL treatment. The central aspect of posterior capsule showed delayed confluence, and the factors related to EMT revealed similar expression patterns to those identified in the cell line. Based on these results, bevacizumab modulates the proliferation and viability of LECs and induces morphological alterations through the modulation of expression patterns of specific factors related to the EMT.Keywords: avastin, bevacizumab, lens epithelial cell, transforming growth factor, vascular endothelial growth factorhttps://www.dovepress.com/effects-of-anti-vascular-endothelial-growth-factor-monoclonal-antibody-peer-reviewed-article-OPTHAvastinbevacizumablens epithelial celltransforming growth factorvascular endothelial growth factor |
| spellingShingle | Jun JH Sohn WJ Lee Y Kim JY Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells Clinical Ophthalmology Avastin bevacizumab lens epithelial cell transforming growth factor vascular endothelial growth factor |
| title | Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells |
| title_full | Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells |
| title_fullStr | Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells |
| title_full_unstemmed | Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells |
| title_short | Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells |
| title_sort | effects of anti vascular endothelial growth factor monoclonal antibody bevacizumab on lens epithelial cells |
| topic | Avastin bevacizumab lens epithelial cell transforming growth factor vascular endothelial growth factor |
| url | https://www.dovepress.com/effects-of-anti-vascular-endothelial-growth-factor-monoclonal-antibody-peer-reviewed-article-OPTH |
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