Discovery of a heat-generated compound DHD derived from Patrinia villosa water extract with inhibitory effects on colon cancer cells viability and migration
Introduction: The plant Patrinia villosa Juss. (PV) has long been used as a medicinal herb for treating intestinal disorders. Pharmacological activities such as anti-oxidation, anti-inflammation, and anti-cancer effects of compounds isolated from PV have been reported, but these bioactive compounds...
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2023.1195883/full |
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| author | Huihai Yang Huihai Yang Tao Zheng Tao Zheng Chuen-Fai Ku Chuen-Fai Ku Cheuk Kit Ngai Grace Gar-Lee Yue Grace Gar-Lee Yue Hung Kay Lee Clara Bik-San Lau Clara Bik-San Lau Clara Bik-San Lau |
| author_facet | Huihai Yang Huihai Yang Tao Zheng Tao Zheng Chuen-Fai Ku Chuen-Fai Ku Cheuk Kit Ngai Grace Gar-Lee Yue Grace Gar-Lee Yue Hung Kay Lee Clara Bik-San Lau Clara Bik-San Lau Clara Bik-San Lau |
| author_sort | Huihai Yang |
| collection | DOAJ |
| description | Introduction: The plant Patrinia villosa Juss. (PV) has long been used as a medicinal herb for treating intestinal disorders. Pharmacological activities such as anti-oxidation, anti-inflammation, and anti-cancer effects of compounds isolated from PV have been reported, but these bioactive compounds were not derived from PV water extract (PVW). Therefore, in the present study, we aimed to identify the active component(s) of PVW which exhibit inhibitory activities in colon cancer cells viability and migration.Methods: Human colon cancer HCT116 cells were treated with the isolated compounds of PVW and then subjected to MTT and transwell migration assays.Results: Our results showed that an active compound in PVW, 8,9-didehydro-7-hydroxydolichodial (DHD) inhibited cell viability of HCT116 cells, with IC50 value at 6.1 ± 2.2 μM. Interestingly, DHD was not detected in the herbal material of PV. Further investigation revealed that DHD is in fact a heat-generated compound derived from a natural compound present in PV, namely valerosidate. Valerosidate also reduced cell viability in HCT116 cells, with IC50 value at 22.2 ± 1.1 μM. Moreover, both DHD (2.75 μM) and valerosidate (10.81 μM) suppressed cell migration in HCT116 cells, with inhibitory rates at 74.8% and 74.6%, respectively. In addition, western blot results showed that DHD (5.5 μM) could significantly increase p53 expression by 34.8% and PTEN expression by 13.9%, while valerosidate (21.6 μM) could increase expressions of p53 and PTEN by 26.1% and 34.6%, respectively in HCT116 cells after 48 h treatment.Discussion: Taken together, this is the first report that a naturally-occurring valerosidate present in PV could actually transform to DHD by thermal hydrolysis, and both compounds exhibited inhibitory effects on cell viability and migration in HCT116 cells via increasing the expressions of tumor suppressors (p53 and PTEN). Our findings demonstrated that valerosidate is present in raw herb PV but not in PVW, while DHD is present in PVW rather than in raw herb PV. This difference in chemical profiles of raw herb and boiled water extract of PV may affect the anti-cancer activity, and hence further investigations are warranted. |
| first_indexed | 2024-03-13T07:55:51Z |
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| language | English |
| last_indexed | 2024-03-13T07:55:51Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-1e7f15ec85854ab0823f739d9a2217a82023-06-02T05:14:39ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462023-06-011110.3389/fchem.2023.11958831195883Discovery of a heat-generated compound DHD derived from Patrinia villosa water extract with inhibitory effects on colon cancer cells viability and migrationHuihai Yang0Huihai Yang1Tao Zheng2Tao Zheng3Chuen-Fai Ku4Chuen-Fai Ku5Cheuk Kit Ngai6Grace Gar-Lee Yue7Grace Gar-Lee Yue8Hung Kay Lee9Clara Bik-San Lau10Clara Bik-San Lau11Clara Bik-San Lau12Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, ChinaState Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, ChinaInstitute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, ChinaState Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, ChinaInstitute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, ChinaState Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, ChinaDepartment of Chemistry, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, ChinaInstitute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, ChinaState Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, ChinaDepartment of Chemistry, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, ChinaInstitute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, ChinaState Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, ChinaLi Dak Sum Yip Yio Chin R&D Centre for Chinese Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, ChinaIntroduction: The plant Patrinia villosa Juss. (PV) has long been used as a medicinal herb for treating intestinal disorders. Pharmacological activities such as anti-oxidation, anti-inflammation, and anti-cancer effects of compounds isolated from PV have been reported, but these bioactive compounds were not derived from PV water extract (PVW). Therefore, in the present study, we aimed to identify the active component(s) of PVW which exhibit inhibitory activities in colon cancer cells viability and migration.Methods: Human colon cancer HCT116 cells were treated with the isolated compounds of PVW and then subjected to MTT and transwell migration assays.Results: Our results showed that an active compound in PVW, 8,9-didehydro-7-hydroxydolichodial (DHD) inhibited cell viability of HCT116 cells, with IC50 value at 6.1 ± 2.2 μM. Interestingly, DHD was not detected in the herbal material of PV. Further investigation revealed that DHD is in fact a heat-generated compound derived from a natural compound present in PV, namely valerosidate. Valerosidate also reduced cell viability in HCT116 cells, with IC50 value at 22.2 ± 1.1 μM. Moreover, both DHD (2.75 μM) and valerosidate (10.81 μM) suppressed cell migration in HCT116 cells, with inhibitory rates at 74.8% and 74.6%, respectively. In addition, western blot results showed that DHD (5.5 μM) could significantly increase p53 expression by 34.8% and PTEN expression by 13.9%, while valerosidate (21.6 μM) could increase expressions of p53 and PTEN by 26.1% and 34.6%, respectively in HCT116 cells after 48 h treatment.Discussion: Taken together, this is the first report that a naturally-occurring valerosidate present in PV could actually transform to DHD by thermal hydrolysis, and both compounds exhibited inhibitory effects on cell viability and migration in HCT116 cells via increasing the expressions of tumor suppressors (p53 and PTEN). Our findings demonstrated that valerosidate is present in raw herb PV but not in PVW, while DHD is present in PVW rather than in raw herb PV. This difference in chemical profiles of raw herb and boiled water extract of PV may affect the anti-cancer activity, and hence further investigations are warranted.https://www.frontiersin.org/articles/10.3389/fchem.2023.1195883/fullPatrinia villosavalerosidatecolon cancerthermal hydrolysis8,9-didehydro-7-hydroxydolichodial |
| spellingShingle | Huihai Yang Huihai Yang Tao Zheng Tao Zheng Chuen-Fai Ku Chuen-Fai Ku Cheuk Kit Ngai Grace Gar-Lee Yue Grace Gar-Lee Yue Hung Kay Lee Clara Bik-San Lau Clara Bik-San Lau Clara Bik-San Lau Discovery of a heat-generated compound DHD derived from Patrinia villosa water extract with inhibitory effects on colon cancer cells viability and migration Frontiers in Chemistry Patrinia villosa valerosidate colon cancer thermal hydrolysis 8,9-didehydro-7-hydroxydolichodial |
| title | Discovery of a heat-generated compound DHD derived from Patrinia villosa water extract with inhibitory effects on colon cancer cells viability and migration |
| title_full | Discovery of a heat-generated compound DHD derived from Patrinia villosa water extract with inhibitory effects on colon cancer cells viability and migration |
| title_fullStr | Discovery of a heat-generated compound DHD derived from Patrinia villosa water extract with inhibitory effects on colon cancer cells viability and migration |
| title_full_unstemmed | Discovery of a heat-generated compound DHD derived from Patrinia villosa water extract with inhibitory effects on colon cancer cells viability and migration |
| title_short | Discovery of a heat-generated compound DHD derived from Patrinia villosa water extract with inhibitory effects on colon cancer cells viability and migration |
| title_sort | discovery of a heat generated compound dhd derived from patrinia villosa water extract with inhibitory effects on colon cancer cells viability and migration |
| topic | Patrinia villosa valerosidate colon cancer thermal hydrolysis 8,9-didehydro-7-hydroxydolichodial |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2023.1195883/full |
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