Prevalence of Extended Spectrum Beta‑Lactamase in Pseudomonas aeruginosa isolated from patients at Sharda Hospital, Greater Noida, Western UP
ABSTRACT Introduction: Pseudomonas aeruginosa is an important pathogen causing healthcare-associated infections especially in immunocompromised patients. It poses a threat to public health due to its inherent resistance to various antimicrobial agents and its ability to acquire new resistance thr...
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| Format: | Article |
| Language: | English |
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University of Science and Technology, Yemen
2022-06-01
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| Series: | Yemeni Journal for Medical Sciences |
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| Online Access: | https://journals.ust.edu/index.php/yjms/article/view/1915 |
| _version_ | 1818248727031185408 |
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| author | Tarana Sarwat Sneha Mohan Satendra P. Singh Dalip K. Kakru |
| author_facet | Tarana Sarwat Sneha Mohan Satendra P. Singh Dalip K. Kakru |
| author_sort | Tarana Sarwat |
| collection | DOAJ |
| description | ABSTRACT
Introduction: Pseudomonas aeruginosa is an important pathogen causing healthcare-associated infections especially in immunocompromised patients. It poses a threat to public health due to its inherent resistance to various antimicrobial agents and its ability to acquire new resistance through multiple mechanisms. Infections due to extended spectrum β-lactamase (ESBL) producing isolates of P. aeruginosa continue to be a challenge for clinicians as these result in high mortality and morbidity due to antimicrobial resistance. The aim of this study was to determine the prevalence of Cefotaximase-Munich (CTX-M) producing strains of P. aeruginosa in Sharda hospital, Greater Noida.
Methods: Strains of P. aeruginosa isolated from various clinical samples were subjected to phenotypic detection for ESBL production by disc combination method. Positive strains were then subjected to polymerase chain reaction (PCR) for detection of blaCTX-M gene.
Results: Out of 166 isolates of P. aeruginosa, 54 (32.53%) were phenotypically confirmed to produce ESBL. Out of these 54 isolates, 39 (72.22%) were positive for blaCTX-M gene. Multidrug resistance was found in 70 (42.17%) isolates. Imipenem was the most effective drug with a sensitivity of 64.86% whereas aztreonam was found to be least effective with sensitivity of only 36.74%.
Conclusion: Current study highlights the phenotypic and molecular characterization of CTX-M gene in P.aeruginosa in our hospital set-up. With judicious use of antimicrobials and strict infection control practices, it might be possible to limit the effect of these drug destroying enzymes.
Keywords: CTX-M gene, Extended spectrum beta lactamases, Hospital acquired infections, multidrug resistance,
Pseudomonas aeruginosa. |
| first_indexed | 2024-12-12T15:25:11Z |
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| id | doaj.art-1e81d3b1853c431292e1d1722ba4241c |
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| issn | 2227-9601 2227-961X |
| language | English |
| last_indexed | 2024-12-12T15:25:11Z |
| publishDate | 2022-06-01 |
| publisher | University of Science and Technology, Yemen |
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| series | Yemeni Journal for Medical Sciences |
| spelling | doaj.art-1e81d3b1853c431292e1d1722ba4241c2022-12-22T00:20:15ZengUniversity of Science and Technology, YemenYemeni Journal for Medical Sciences2227-96012227-961X2022-06-0116110.20428/YJMS.16.1.A11687Prevalence of Extended Spectrum Beta‑Lactamase in Pseudomonas aeruginosa isolated from patients at Sharda Hospital, Greater Noida, Western UPTarana Sarwat0Sneha Mohan1Satendra P. Singh2Dalip K. Kakru3Department of Microbiology, Sharda University, Greater NoidaDepartment of Microbiology, Sharda University, Greater NoidaDepartment of Microbiology, Sharda University, Greater NoidaDepartment of Microbiology, Sharda University, Greater Noida.ABSTRACT Introduction: Pseudomonas aeruginosa is an important pathogen causing healthcare-associated infections especially in immunocompromised patients. It poses a threat to public health due to its inherent resistance to various antimicrobial agents and its ability to acquire new resistance through multiple mechanisms. Infections due to extended spectrum β-lactamase (ESBL) producing isolates of P. aeruginosa continue to be a challenge for clinicians as these result in high mortality and morbidity due to antimicrobial resistance. The aim of this study was to determine the prevalence of Cefotaximase-Munich (CTX-M) producing strains of P. aeruginosa in Sharda hospital, Greater Noida. Methods: Strains of P. aeruginosa isolated from various clinical samples were subjected to phenotypic detection for ESBL production by disc combination method. Positive strains were then subjected to polymerase chain reaction (PCR) for detection of blaCTX-M gene. Results: Out of 166 isolates of P. aeruginosa, 54 (32.53%) were phenotypically confirmed to produce ESBL. Out of these 54 isolates, 39 (72.22%) were positive for blaCTX-M gene. Multidrug resistance was found in 70 (42.17%) isolates. Imipenem was the most effective drug with a sensitivity of 64.86% whereas aztreonam was found to be least effective with sensitivity of only 36.74%. Conclusion: Current study highlights the phenotypic and molecular characterization of CTX-M gene in P.aeruginosa in our hospital set-up. With judicious use of antimicrobials and strict infection control practices, it might be possible to limit the effect of these drug destroying enzymes. Keywords: CTX-M gene, Extended spectrum beta lactamases, Hospital acquired infections, multidrug resistance, Pseudomonas aeruginosa.https://journals.ust.edu/index.php/yjms/article/view/1915ctx-m gene, extended spectrum beta lactamases, hospital acquired infections, multidrug resistance, pseudomonas aeruginosa |
| spellingShingle | Tarana Sarwat Sneha Mohan Satendra P. Singh Dalip K. Kakru Prevalence of Extended Spectrum Beta‑Lactamase in Pseudomonas aeruginosa isolated from patients at Sharda Hospital, Greater Noida, Western UP Yemeni Journal for Medical Sciences ctx-m gene, extended spectrum beta lactamases, hospital acquired infections, multidrug resistance, pseudomonas aeruginosa |
| title | Prevalence of Extended Spectrum Beta‑Lactamase in Pseudomonas aeruginosa isolated from patients at Sharda Hospital, Greater Noida, Western UP |
| title_full | Prevalence of Extended Spectrum Beta‑Lactamase in Pseudomonas aeruginosa isolated from patients at Sharda Hospital, Greater Noida, Western UP |
| title_fullStr | Prevalence of Extended Spectrum Beta‑Lactamase in Pseudomonas aeruginosa isolated from patients at Sharda Hospital, Greater Noida, Western UP |
| title_full_unstemmed | Prevalence of Extended Spectrum Beta‑Lactamase in Pseudomonas aeruginosa isolated from patients at Sharda Hospital, Greater Noida, Western UP |
| title_short | Prevalence of Extended Spectrum Beta‑Lactamase in Pseudomonas aeruginosa isolated from patients at Sharda Hospital, Greater Noida, Western UP |
| title_sort | prevalence of extended spectrum beta lactamase in pseudomonas aeruginosa isolated from patients at sharda hospital greater noida western up |
| topic | ctx-m gene, extended spectrum beta lactamases, hospital acquired infections, multidrug resistance, pseudomonas aeruginosa |
| url | https://journals.ust.edu/index.php/yjms/article/view/1915 |
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