Bacteriophage 434 Hex Protein Prevents RecA-Mediated Repressor Autocleavage
In a λimm434 lysogen, two proteins are expressed from the integrated prophage. Both are encoded by the same mRNA whose transcription initiates at the PRM promoter. One protein is the 434 repressor, needed for the establishment and maintenance of lysogeny. The other is Hex which is trans...
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| Format: | Article |
| Language: | English |
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MDPI AG
2013-01-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/5/1/111 |
| _version_ | 1818917422042710016 |
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| author | Paul Shkilnyj Michael P. Colon Gerald B. Koudelka |
| author_facet | Paul Shkilnyj Michael P. Colon Gerald B. Koudelka |
| author_sort | Paul Shkilnyj |
| collection | DOAJ |
| description | In a λimm434 lysogen, two proteins are expressed from the integrated prophage. Both are encoded by the same mRNA whose transcription initiates at the PRM promoter. One protein is the 434 repressor, needed for the establishment and maintenance of lysogeny. The other is Hex which is translated from an open reading frame that apparently partially overlaps the 434 repressor coding region. In the wild type host, disruption of the gene encoding Hex destabilizes λimm434 lysogens. However, the hex mutation has no effect on lysogen stability in a recA− host. These observations suggest that Hex functions by modulating the ability of RecA to stimulate 434 repressor autocleavage. We tested this hypothesis by identifying and purifying Hex to determine if this protein inhibited RecA‑stimulated autocleavage of 434 repressor in vitro. Our results show that in vitro a fragment of Hex prevents RecA-stimulated autocleavage of 434 repressor, as well as the repressors of the closely related phage P22. Surprisingly, Hex does not prevent RecA‑stimulated autocleavage of phage lambda repressor, nor the E. coli LexA repressor. |
| first_indexed | 2024-12-20T00:33:49Z |
| format | Article |
| id | doaj.art-1e82aa30da444b6fa0e68e9ea628c58b |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-12-20T00:33:49Z |
| publishDate | 2013-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-1e82aa30da444b6fa0e68e9ea628c58b2022-12-21T19:59:50ZengMDPI AGViruses1999-49152013-01-015111112610.3390/v5010111Bacteriophage 434 Hex Protein Prevents RecA-Mediated Repressor AutocleavagePaul ShkilnyjMichael P. ColonGerald B. KoudelkaIn a λimm434 lysogen, two proteins are expressed from the integrated prophage. Both are encoded by the same mRNA whose transcription initiates at the PRM promoter. One protein is the 434 repressor, needed for the establishment and maintenance of lysogeny. The other is Hex which is translated from an open reading frame that apparently partially overlaps the 434 repressor coding region. In the wild type host, disruption of the gene encoding Hex destabilizes λimm434 lysogens. However, the hex mutation has no effect on lysogen stability in a recA− host. These observations suggest that Hex functions by modulating the ability of RecA to stimulate 434 repressor autocleavage. We tested this hypothesis by identifying and purifying Hex to determine if this protein inhibited RecA‑stimulated autocleavage of 434 repressor in vitro. Our results show that in vitro a fragment of Hex prevents RecA-stimulated autocleavage of 434 repressor, as well as the repressors of the closely related phage P22. Surprisingly, Hex does not prevent RecA‑stimulated autocleavage of phage lambda repressor, nor the E. coli LexA repressor.http://www.mdpi.com/1999-4915/5/1/111bacteriophageRecAlysogeny |
| spellingShingle | Paul Shkilnyj Michael P. Colon Gerald B. Koudelka Bacteriophage 434 Hex Protein Prevents RecA-Mediated Repressor Autocleavage Viruses bacteriophage RecA lysogeny |
| title | Bacteriophage 434 Hex Protein Prevents RecA-Mediated Repressor Autocleavage |
| title_full | Bacteriophage 434 Hex Protein Prevents RecA-Mediated Repressor Autocleavage |
| title_fullStr | Bacteriophage 434 Hex Protein Prevents RecA-Mediated Repressor Autocleavage |
| title_full_unstemmed | Bacteriophage 434 Hex Protein Prevents RecA-Mediated Repressor Autocleavage |
| title_short | Bacteriophage 434 Hex Protein Prevents RecA-Mediated Repressor Autocleavage |
| title_sort | bacteriophage 434 hex protein prevents reca mediated repressor autocleavage |
| topic | bacteriophage RecA lysogeny |
| url | http://www.mdpi.com/1999-4915/5/1/111 |
| work_keys_str_mv | AT paulshkilnyj bacteriophage434hexproteinpreventsrecamediatedrepressorautocleavage AT michaelpcolon bacteriophage434hexproteinpreventsrecamediatedrepressorautocleavage AT geraldbkoudelka bacteriophage434hexproteinpreventsrecamediatedrepressorautocleavage |