Tropomyosin: An Excretory/Secretory Protein from <i>Haemonchus contortus</i> Mediates the Immuno-Suppressive Potential of Goat Peripheral Blood Mononuclear Cells In Vitro
During host-parasite interactions, binding of excretory/secretory proteins (ESPs) on the host immune cells is considered the fundamental phase for regulation of immune responses. In this study, gene encoding <i>Haemonchus contortus </i>tropomyosin (Hc-TpMy), was successfully cloned and e...
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MDPI AG
2020-03-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/8/1/109 |
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| author | Muhammad Ehsan Muhammad Haseeb Ruisi Hu Haider Ali Muhammad Ali Memon Ruofeng Yan Lixin Xu Xiaokai Song Xingquan Zhu Xiangrui Li |
| author_facet | Muhammad Ehsan Muhammad Haseeb Ruisi Hu Haider Ali Muhammad Ali Memon Ruofeng Yan Lixin Xu Xiaokai Song Xingquan Zhu Xiangrui Li |
| author_sort | Muhammad Ehsan |
| collection | DOAJ |
| description | During host-parasite interactions, binding of excretory/secretory proteins (ESPs) on the host immune cells is considered the fundamental phase for regulation of immune responses. In this study, gene encoding <i>Haemonchus contortus </i>tropomyosin (Hc-TpMy), was successfully cloned and expressed, and the recombinant protein after host cell surface attachment was evaluated for immune functional analysis with goat peripheral blood mononuclear cells (PBMCs) in vitro. The isopropyl-β-D-thiogalactopyranoside (IPTG)-induced recombinant protein was successfully recognized by the sera of rat experimentally infected with rHc-TpMy. The immunofluorescence assay detected attachment of rHc-TpMy on the surface of host PBMCs. Furthermore, immunoregulatory roles of rHc-TpMy on cytokines expression, PBMC proliferation, migration, nitric oxide (NO) production, apoptosis and monocytes phagocytosis were observed. The results showed that expression of IL-4 and IFN-γ cytokines, cell proliferation, NO production and PBMC migration were significantly suppressed by goat PBMCs after co-incubation with rHc-TpMy protein. However, the productions of IL-10, IL-17 and TGF-β1 cytokines, PBMCs apoptosis and monocytes phagocytosis were elevated at dose dependent manner. Our findings indicated that rHc-TpMy is an important ES binding protein exhibit distinct immuno-suppressive roles on goat PBMCs which might be a potential molecular target to control haemonchosis in future. |
| first_indexed | 2024-04-13T06:51:01Z |
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| id | doaj.art-1e82aa3db7624f3fb19617d3b260f1ae |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-04-13T06:51:01Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
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| series | Vaccines |
| spelling | doaj.art-1e82aa3db7624f3fb19617d3b260f1ae2022-12-22T02:57:24ZengMDPI AGVaccines2076-393X2020-03-018110910.3390/vaccines8010109vaccines8010109Tropomyosin: An Excretory/Secretory Protein from <i>Haemonchus contortus</i> Mediates the Immuno-Suppressive Potential of Goat Peripheral Blood Mononuclear Cells In VitroMuhammad Ehsan0Muhammad Haseeb1Ruisi Hu2Haider Ali3Muhammad Ali Memon4Ruofeng Yan5Lixin Xu6Xiaokai Song7Xingquan Zhu8Xiangrui Li9MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, ChinaMOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, Jiangsu, ChinaDuring host-parasite interactions, binding of excretory/secretory proteins (ESPs) on the host immune cells is considered the fundamental phase for regulation of immune responses. In this study, gene encoding <i>Haemonchus contortus </i>tropomyosin (Hc-TpMy), was successfully cloned and expressed, and the recombinant protein after host cell surface attachment was evaluated for immune functional analysis with goat peripheral blood mononuclear cells (PBMCs) in vitro. The isopropyl-β-D-thiogalactopyranoside (IPTG)-induced recombinant protein was successfully recognized by the sera of rat experimentally infected with rHc-TpMy. The immunofluorescence assay detected attachment of rHc-TpMy on the surface of host PBMCs. Furthermore, immunoregulatory roles of rHc-TpMy on cytokines expression, PBMC proliferation, migration, nitric oxide (NO) production, apoptosis and monocytes phagocytosis were observed. The results showed that expression of IL-4 and IFN-γ cytokines, cell proliferation, NO production and PBMC migration were significantly suppressed by goat PBMCs after co-incubation with rHc-TpMy protein. However, the productions of IL-10, IL-17 and TGF-β1 cytokines, PBMCs apoptosis and monocytes phagocytosis were elevated at dose dependent manner. Our findings indicated that rHc-TpMy is an important ES binding protein exhibit distinct immuno-suppressive roles on goat PBMCs which might be a potential molecular target to control haemonchosis in future.https://www.mdpi.com/2076-393X/8/1/109<i>haemonchus contortus</i>tropomyosingoatpbmcshost-parasite interactionsimmune responses |
| spellingShingle | Muhammad Ehsan Muhammad Haseeb Ruisi Hu Haider Ali Muhammad Ali Memon Ruofeng Yan Lixin Xu Xiaokai Song Xingquan Zhu Xiangrui Li Tropomyosin: An Excretory/Secretory Protein from <i>Haemonchus contortus</i> Mediates the Immuno-Suppressive Potential of Goat Peripheral Blood Mononuclear Cells In Vitro Vaccines <i>haemonchus contortus</i> tropomyosin goat pbmcs host-parasite interactions immune responses |
| title | Tropomyosin: An Excretory/Secretory Protein from <i>Haemonchus contortus</i> Mediates the Immuno-Suppressive Potential of Goat Peripheral Blood Mononuclear Cells In Vitro |
| title_full | Tropomyosin: An Excretory/Secretory Protein from <i>Haemonchus contortus</i> Mediates the Immuno-Suppressive Potential of Goat Peripheral Blood Mononuclear Cells In Vitro |
| title_fullStr | Tropomyosin: An Excretory/Secretory Protein from <i>Haemonchus contortus</i> Mediates the Immuno-Suppressive Potential of Goat Peripheral Blood Mononuclear Cells In Vitro |
| title_full_unstemmed | Tropomyosin: An Excretory/Secretory Protein from <i>Haemonchus contortus</i> Mediates the Immuno-Suppressive Potential of Goat Peripheral Blood Mononuclear Cells In Vitro |
| title_short | Tropomyosin: An Excretory/Secretory Protein from <i>Haemonchus contortus</i> Mediates the Immuno-Suppressive Potential of Goat Peripheral Blood Mononuclear Cells In Vitro |
| title_sort | tropomyosin an excretory secretory protein from i haemonchus contortus i mediates the immuno suppressive potential of goat peripheral blood mononuclear cells in vitro |
| topic | <i>haemonchus contortus</i> tropomyosin goat pbmcs host-parasite interactions immune responses |
| url | https://www.mdpi.com/2076-393X/8/1/109 |
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