Comparison of Fecal Collection Methods on Variation in Gut Metagenomics and Untargeted Metabolomics
The choice of fecal collection method is essential for studying gut microbe-human interactions in large-scale population-based research. In this study, we examined the effects of fecal collection methods and storage time at ambient temperature on variations in the gut microbiome community compositio...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2021-10-01
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| Series: | mSphere |
| Online Access: | https://journals.asm.org/doi/10.1128/mSphere.00636-21 |
| _version_ | 1818384262853820416 |
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| author | Huihui Guan Yanni Pu Chenglin Liu Tao Lou Shishang Tan Mengmeng Kong Zhonghan Sun Zhendong Mei Qibin Qi Zhexue Quan Guoping Zhao Yan Zheng |
| author_facet | Huihui Guan Yanni Pu Chenglin Liu Tao Lou Shishang Tan Mengmeng Kong Zhonghan Sun Zhendong Mei Qibin Qi Zhexue Quan Guoping Zhao Yan Zheng |
| author_sort | Huihui Guan |
| collection | DOAJ |
| description | The choice of fecal collection method is essential for studying gut microbe-human interactions in large-scale population-based research. In this study, we examined the effects of fecal collection methods and storage time at ambient temperature on variations in the gut microbiome community composition; microbial diversity metrics at the species, gene, and pathway levels; antibiotic resistance genes; and metabolome profiling. |
| first_indexed | 2024-12-14T03:19:28Z |
| format | Article |
| id | doaj.art-1e83845c222e480a8ffbbcbd8c3de4e0 |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-14T03:19:28Z |
| publishDate | 2021-10-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-1e83845c222e480a8ffbbcbd8c3de4e02022-12-21T23:19:04ZengAmerican Society for MicrobiologymSphere2379-50422021-10-016510.1128/mSphere.00636-21Comparison of Fecal Collection Methods on Variation in Gut Metagenomics and Untargeted MetabolomicsHuihui Guan0Yanni Pu1Chenglin Liu2Tao Lou3Shishang Tan4Mengmeng Kong5Zhonghan Sun6Zhendong Mei7Qibin Qi8Zhexue Quan9https://orcid.org/0000-0002-7561-8572Guoping Zhao10Yan Zheng11https://orcid.org/0000-0003-1129-3147Human Phenome Institute, School of Life Sciences, Fudan University, Shanghai, ChinaHuman Phenome Institute, School of Life Sciences, Fudan University, Shanghai, ChinaHuman Phenome Institute, School of Life Sciences, Fudan University, Shanghai, ChinaHuman Phenome Institute, School of Life Sciences, Fudan University, Shanghai, ChinaHuman Phenome Institute, School of Life Sciences, Fudan University, Shanghai, ChinaHuman Phenome Institute, School of Life Sciences, Fudan University, Shanghai, ChinaHuman Phenome Institute, School of Life Sciences, Fudan University, Shanghai, ChinaHuman Phenome Institute, School of Life Sciences, Fudan University, Shanghai, ChinaDepartment of Epidemiology and Population Health, Albert Einstein College of Medicine, Bronx, New York, USAMinistry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Institute of Biodiversity Science, School of Life Sciences, Fudan University, Shanghai, ChinaInstitute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaState Key Laboratory of Genetic Engineering School of Life Sciences, Human Phenome Institute, Fudan University, Shanghai, ChinaThe choice of fecal collection method is essential for studying gut microbe-human interactions in large-scale population-based research. In this study, we examined the effects of fecal collection methods and storage time at ambient temperature on variations in the gut microbiome community composition; microbial diversity metrics at the species, gene, and pathway levels; antibiotic resistance genes; and metabolome profiling.https://journals.asm.org/doi/10.1128/mSphere.00636-21 |
| spellingShingle | Huihui Guan Yanni Pu Chenglin Liu Tao Lou Shishang Tan Mengmeng Kong Zhonghan Sun Zhendong Mei Qibin Qi Zhexue Quan Guoping Zhao Yan Zheng Comparison of Fecal Collection Methods on Variation in Gut Metagenomics and Untargeted Metabolomics mSphere |
| title | Comparison of Fecal Collection Methods on Variation in Gut Metagenomics and Untargeted Metabolomics |
| title_full | Comparison of Fecal Collection Methods on Variation in Gut Metagenomics and Untargeted Metabolomics |
| title_fullStr | Comparison of Fecal Collection Methods on Variation in Gut Metagenomics and Untargeted Metabolomics |
| title_full_unstemmed | Comparison of Fecal Collection Methods on Variation in Gut Metagenomics and Untargeted Metabolomics |
| title_short | Comparison of Fecal Collection Methods on Variation in Gut Metagenomics and Untargeted Metabolomics |
| title_sort | comparison of fecal collection methods on variation in gut metagenomics and untargeted metabolomics |
| url | https://journals.asm.org/doi/10.1128/mSphere.00636-21 |
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