Sputum Microbiota in Coal Workers Diagnosed with Pneumoconiosis as Revealed by 16S rRNA Gene Sequencing
Coal worker’s pneumoconiosis (CWP) is an occupationally induced progressive fibrotic lung disease. This irreversible but preventable disease currently affects millions across the world, mainly in countries with developed coal mining industries. Here, we report a pilot study that explores the sputum...
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MDPI AG
2022-06-01
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| Online Access: | https://www.mdpi.com/2075-1729/12/6/830 |
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| author | Vladimir G. Druzhinin Elizaveta D. Baranova Ludmila V. Matskova Pavel S. Demenkov Valentin P. Volobaev Varvara I. Minina Alexey V. Larionov Snezana A. Paradnikova |
| author_facet | Vladimir G. Druzhinin Elizaveta D. Baranova Ludmila V. Matskova Pavel S. Demenkov Valentin P. Volobaev Varvara I. Minina Alexey V. Larionov Snezana A. Paradnikova |
| author_sort | Vladimir G. Druzhinin |
| collection | DOAJ |
| description | Coal worker’s pneumoconiosis (CWP) is an occupationally induced progressive fibrotic lung disease. This irreversible but preventable disease currently affects millions across the world, mainly in countries with developed coal mining industries. Here, we report a pilot study that explores the sputum microbiome as a potential non-invasive bacterial biomarker of CWP status. Sputum samples were collected from 35 former and active coal miners diagnosed with CWP and 35 healthy controls. Sequencing of bacterial 16S rRNA genes was used to study the taxonomic composition of the respiratory microbiome. There was no difference in alpha diversity between CWP and controls. The structure of bacterial communities in sputum samples (β diversity) differed significantly between cases and controls (pseudo-F = 3.61; <i>p</i> = 0.004). A significant increase in the abundance of <i>Streptococcus</i> (25.12 ± 11.37 vs. 16.85 ± 11.35%; <i>p</i> = 0.0003) was detected in samples from CWP subjects as compared to controls. The increased representation of <i>Streptococcus</i> in sputum from CWP patients was associated only with the presence of occupational pulmonary fibrosis, but did not depend on age, and did not differ between former and current miners. The study shows, for the first time, that the sputum microbiota of CWP subjects differs from that of controls. The results of our present exploratory study warrant further investigations on a larger cohort. |
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| language | English |
| last_indexed | 2024-03-09T23:15:20Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
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| series | Life |
| spelling | doaj.art-1e8e2248052341c48eaf912e3e8561e52023-11-23T17:36:09ZengMDPI AGLife2075-17292022-06-0112683010.3390/life12060830Sputum Microbiota in Coal Workers Diagnosed with Pneumoconiosis as Revealed by 16S rRNA Gene SequencingVladimir G. Druzhinin0Elizaveta D. Baranova1Ludmila V. Matskova2Pavel S. Demenkov3Valentin P. Volobaev4Varvara I. Minina5Alexey V. Larionov6Snezana A. Paradnikova7Department of Genetics and Fundamental Medicine, Kemerovo State University, 650000 Kemerovo, RussiaDepartment of Genetics and Fundamental Medicine, Kemerovo State University, 650000 Kemerovo, RussiaInstitute of Living Systems, Immanuel Kant Baltic Federal University, 236016 Kaliningrad, RussiaInstitute of Cytology and Genetics SB RAS, 630090 Novosibirsk, RussiaCenter for Genetics and Life Sciences, Sirius University of Science and Technology, 354340 Sochi, RussiaDepartment of Genetics and Fundamental Medicine, Kemerovo State University, 650000 Kemerovo, RussiaDepartment of Genetics and Fundamental Medicine, Kemerovo State University, 650000 Kemerovo, RussiaDepartment of Genetics and Fundamental Medicine, Kemerovo State University, 650000 Kemerovo, RussiaCoal worker’s pneumoconiosis (CWP) is an occupationally induced progressive fibrotic lung disease. This irreversible but preventable disease currently affects millions across the world, mainly in countries with developed coal mining industries. Here, we report a pilot study that explores the sputum microbiome as a potential non-invasive bacterial biomarker of CWP status. Sputum samples were collected from 35 former and active coal miners diagnosed with CWP and 35 healthy controls. Sequencing of bacterial 16S rRNA genes was used to study the taxonomic composition of the respiratory microbiome. There was no difference in alpha diversity between CWP and controls. The structure of bacterial communities in sputum samples (β diversity) differed significantly between cases and controls (pseudo-F = 3.61; <i>p</i> = 0.004). A significant increase in the abundance of <i>Streptococcus</i> (25.12 ± 11.37 vs. 16.85 ± 11.35%; <i>p</i> = 0.0003) was detected in samples from CWP subjects as compared to controls. The increased representation of <i>Streptococcus</i> in sputum from CWP patients was associated only with the presence of occupational pulmonary fibrosis, but did not depend on age, and did not differ between former and current miners. The study shows, for the first time, that the sputum microbiota of CWP subjects differs from that of controls. The results of our present exploratory study warrant further investigations on a larger cohort.https://www.mdpi.com/2075-1729/12/6/830coal worker’s pneumoconiosissputum microbiomelung fibrosisnext-generation sequencing16S rRNA genes<i>Streptococcus</i> |
| spellingShingle | Vladimir G. Druzhinin Elizaveta D. Baranova Ludmila V. Matskova Pavel S. Demenkov Valentin P. Volobaev Varvara I. Minina Alexey V. Larionov Snezana A. Paradnikova Sputum Microbiota in Coal Workers Diagnosed with Pneumoconiosis as Revealed by 16S rRNA Gene Sequencing Life coal worker’s pneumoconiosis sputum microbiome lung fibrosis next-generation sequencing 16S rRNA genes <i>Streptococcus</i> |
| title | Sputum Microbiota in Coal Workers Diagnosed with Pneumoconiosis as Revealed by 16S rRNA Gene Sequencing |
| title_full | Sputum Microbiota in Coal Workers Diagnosed with Pneumoconiosis as Revealed by 16S rRNA Gene Sequencing |
| title_fullStr | Sputum Microbiota in Coal Workers Diagnosed with Pneumoconiosis as Revealed by 16S rRNA Gene Sequencing |
| title_full_unstemmed | Sputum Microbiota in Coal Workers Diagnosed with Pneumoconiosis as Revealed by 16S rRNA Gene Sequencing |
| title_short | Sputum Microbiota in Coal Workers Diagnosed with Pneumoconiosis as Revealed by 16S rRNA Gene Sequencing |
| title_sort | sputum microbiota in coal workers diagnosed with pneumoconiosis as revealed by 16s rrna gene sequencing |
| topic | coal worker’s pneumoconiosis sputum microbiome lung fibrosis next-generation sequencing 16S rRNA genes <i>Streptococcus</i> |
| url | https://www.mdpi.com/2075-1729/12/6/830 |
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