A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au
Abstract Carbon dots and Fe3O4@Au were synthesized to develop a new biosensor to detect DNA target. We investigated the photoluminescence property of carbon dots (CDs) in the presence of Fe3O4-capped Au (Fe3O4@Au). Firstly, we designed two dedicated probes for unique long sequence region of human T-...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2018-10-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-018-32756-w |
| _version_ | 1818995986183225344 |
|---|---|
| author | Mohadeseh Zarei-Ghobadi Sayed-Hamidreza Mozhgani Fariba Dashtestani Amir Yadegari Fatemeh Hakimian Mehdi Norouzi Hedayatollah Ghourchian |
| author_facet | Mohadeseh Zarei-Ghobadi Sayed-Hamidreza Mozhgani Fariba Dashtestani Amir Yadegari Fatemeh Hakimian Mehdi Norouzi Hedayatollah Ghourchian |
| author_sort | Mohadeseh Zarei-Ghobadi |
| collection | DOAJ |
| description | Abstract Carbon dots and Fe3O4@Au were synthesized to develop a new biosensor to detect DNA target. We investigated the photoluminescence property of carbon dots (CDs) in the presence of Fe3O4-capped Au (Fe3O4@Au). Firstly, we designed two dedicated probes for unique long sequence region of human T-lymphotropic virus type 1 genome. One of the probes was covalently bound to the CDs. In the absence of target, CDs-probe was adsorbed on the surface of Fe3O4@Au through two possible mechanisms, leading to quenching the fluorescence emission of CDs. The fluorescence emission of CDs was recovered in the presence of target since double-stranded DNA cannot adsorb on the Fe3O4@Au. Also, Fe3O4@Au can adsorb the unhybridized oligonucleotides and improves the accuracy of detection. The specificity of the proposed biosensor was confirmed by BLAST search and assessed by exposing the biosensor to other virus targets. The experimental detection limit of the biosensor was below 10 nM with linear range from 10 to 320 nM. |
| first_indexed | 2024-12-20T21:22:33Z |
| format | Article |
| id | doaj.art-1e8f3db8911e4b82971b69174923d6bb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T21:22:33Z |
| publishDate | 2018-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1e8f3db8911e4b82971b69174923d6bb2022-12-21T19:26:14ZengNature PortfolioScientific Reports2045-23222018-10-01811810.1038/s41598-018-32756-wA genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped AuMohadeseh Zarei-Ghobadi0Sayed-Hamidreza Mozhgani1Fariba Dashtestani2Amir Yadegari3Fatemeh Hakimian4Mehdi Norouzi5Hedayatollah Ghourchian6Institute of Biochemistry and Biophysics, University of TehranDepartment of Microbiology, School of Medicine, Alborz University of Medical SciencesInstitute of Biochemistry and Biophysics, University of TehranDepartment of Developmental Sciences, Marquette University School of DentistryInstitute of Biochemistry and Biophysics, University of TehranDepartment of Virology, School of Public Health, Tehran University of Medical SciencesInstitute of Biochemistry and Biophysics, University of TehranAbstract Carbon dots and Fe3O4@Au were synthesized to develop a new biosensor to detect DNA target. We investigated the photoluminescence property of carbon dots (CDs) in the presence of Fe3O4-capped Au (Fe3O4@Au). Firstly, we designed two dedicated probes for unique long sequence region of human T-lymphotropic virus type 1 genome. One of the probes was covalently bound to the CDs. In the absence of target, CDs-probe was adsorbed on the surface of Fe3O4@Au through two possible mechanisms, leading to quenching the fluorescence emission of CDs. The fluorescence emission of CDs was recovered in the presence of target since double-stranded DNA cannot adsorb on the Fe3O4@Au. Also, Fe3O4@Au can adsorb the unhybridized oligonucleotides and improves the accuracy of detection. The specificity of the proposed biosensor was confirmed by BLAST search and assessed by exposing the biosensor to other virus targets. The experimental detection limit of the biosensor was below 10 nM with linear range from 10 to 320 nM.https://doi.org/10.1038/s41598-018-32756-wCarbon Dots (CDs)CDs FluorescenceBiosensorsFRET EfficiencyFörster Resonance Energy Transfer (FRET) |
| spellingShingle | Mohadeseh Zarei-Ghobadi Sayed-Hamidreza Mozhgani Fariba Dashtestani Amir Yadegari Fatemeh Hakimian Mehdi Norouzi Hedayatollah Ghourchian A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au Scientific Reports Carbon Dots (CDs) CDs Fluorescence Biosensors FRET Efficiency Förster Resonance Energy Transfer (FRET) |
| title | A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au |
| title_full | A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au |
| title_fullStr | A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au |
| title_full_unstemmed | A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au |
| title_short | A genosensor for detection of HTLV-I based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle-capped Au |
| title_sort | genosensor for detection of htlv i based on photoluminescence quenching of fluorescent carbon dots in presence of iron magnetic nanoparticle capped au |
| topic | Carbon Dots (CDs) CDs Fluorescence Biosensors FRET Efficiency Förster Resonance Energy Transfer (FRET) |
| url | https://doi.org/10.1038/s41598-018-32756-w |
| work_keys_str_mv | AT mohadesehzareighobadi agenosensorfordetectionofhtlvibasedonphotoluminescencequenchingoffluorescentcarbondotsinpresenceofironmagneticnanoparticlecappedau AT sayedhamidrezamozhgani agenosensorfordetectionofhtlvibasedonphotoluminescencequenchingoffluorescentcarbondotsinpresenceofironmagneticnanoparticlecappedau AT faribadashtestani agenosensorfordetectionofhtlvibasedonphotoluminescencequenchingoffluorescentcarbondotsinpresenceofironmagneticnanoparticlecappedau AT amiryadegari agenosensorfordetectionofhtlvibasedonphotoluminescencequenchingoffluorescentcarbondotsinpresenceofironmagneticnanoparticlecappedau AT fatemehhakimian agenosensorfordetectionofhtlvibasedonphotoluminescencequenchingoffluorescentcarbondotsinpresenceofironmagneticnanoparticlecappedau AT mehdinorouzi agenosensorfordetectionofhtlvibasedonphotoluminescencequenchingoffluorescentcarbondotsinpresenceofironmagneticnanoparticlecappedau AT hedayatollahghourchian agenosensorfordetectionofhtlvibasedonphotoluminescencequenchingoffluorescentcarbondotsinpresenceofironmagneticnanoparticlecappedau AT mohadesehzareighobadi genosensorfordetectionofhtlvibasedonphotoluminescencequenchingoffluorescentcarbondotsinpresenceofironmagneticnanoparticlecappedau AT sayedhamidrezamozhgani genosensorfordetectionofhtlvibasedonphotoluminescencequenchingoffluorescentcarbondotsinpresenceofironmagneticnanoparticlecappedau AT faribadashtestani genosensorfordetectionofhtlvibasedonphotoluminescencequenchingoffluorescentcarbondotsinpresenceofironmagneticnanoparticlecappedau AT amiryadegari genosensorfordetectionofhtlvibasedonphotoluminescencequenchingoffluorescentcarbondotsinpresenceofironmagneticnanoparticlecappedau AT fatemehhakimian genosensorfordetectionofhtlvibasedonphotoluminescencequenchingoffluorescentcarbondotsinpresenceofironmagneticnanoparticlecappedau AT mehdinorouzi genosensorfordetectionofhtlvibasedonphotoluminescencequenchingoffluorescentcarbondotsinpresenceofironmagneticnanoparticlecappedau AT hedayatollahghourchian genosensorfordetectionofhtlvibasedonphotoluminescencequenchingoffluorescentcarbondotsinpresenceofironmagneticnanoparticlecappedau |