Sinisan ameliorates colonic injury induced by water immersion restraint stress by enhancing intestinal barrier function and the gut microbiota structure
AbstractContext Sinisan (SNS) has been used to treat psychosomatic diseases of the digestive system. But little is known about how SNS affects water immersion restraint stress (WIRS).Objective To study the effects of SNS on colonic tissue injury in the WIRS model.Materials and methods Forty-eight Ku...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Pharmaceutical Biology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2023.2191643 |
| _version_ | 1797234814490771456 |
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| author | Xiaoying Xu Huimei Hu Haizhou Zeng Boyi Li Qiuxiong Yin Yupeng Jiang Linquan Zang Changlin Zhao Guoqiang Qian |
| author_facet | Xiaoying Xu Huimei Hu Haizhou Zeng Boyi Li Qiuxiong Yin Yupeng Jiang Linquan Zang Changlin Zhao Guoqiang Qian |
| author_sort | Xiaoying Xu |
| collection | DOAJ |
| description | AbstractContext Sinisan (SNS) has been used to treat psychosomatic diseases of the digestive system. But little is known about how SNS affects water immersion restraint stress (WIRS).Objective To study the effects of SNS on colonic tissue injury in the WIRS model.Materials and methods Forty-eight Kunming (KM) mice were randomized into 6 groups (n = 8): The control and WIRS groups receiving deionized water; the SNS low-dose (SL, 3.12 g/kg/d), SNS middle-dose (SM, 6.24 g/kg/d), SNS high-dose (SH, 12.48 g/kg/d), and diazepam (DZ, 5 mg/kg/d) groups; each with two daily administrations for 5 consecutive days. The 5 treatment groups were subjected to WIRS for 24 h on day 6. The effects of SNS on colon tissue injury caused by WIRS were assessed by changes in colon histology, inflammatory cytokines, brain-gut peptides, and tight junction (TJ) proteins levels. 16S rRNA gene sequencing was used to detect the regulation of the gut microbiota.Results SNS pretreatment significantly reduced TNF-α (0.75- to 0.81-fold), IL-6 (0.77-fold), and IFN-γ (0.69-fold) levels; and increased TJ proteins levels, such as ZO-1 (4.06- to 5.27-fold), claudin-1 (3.33- to 5.14-fold), and occludin (6.46- to 11.82-fold). However, there was no significant difference between the levels of substance P (SP) and vasoactive intestinal peptide (VIP) in the control and WIRS groups. SNS regulated the composition of gut microbiota in WIRS mice.Conclusion The positive effects of SNS on WIRS could provide a theoretical basis to treat stress-related gastrointestinal disorders. |
| first_indexed | 2024-03-09T00:08:51Z |
| format | Article |
| id | doaj.art-1e8f5aa4836743478d257c332fb9c31e |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-04-24T16:38:03Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-1e8f5aa4836743478d257c332fb9c31e2024-03-29T11:10:26ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162023-12-0161159860910.1080/13880209.2023.2191643Sinisan ameliorates colonic injury induced by water immersion restraint stress by enhancing intestinal barrier function and the gut microbiota structureXiaoying Xu0Huimei Hu1Haizhou Zeng2Boyi Li3Qiuxiong Yin4Yupeng Jiang5Linquan Zang6Changlin Zhao7Guoqiang Qian8School of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. ChinaSchool of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. ChinaSchool of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. ChinaSchool of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. ChinaSchool of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. ChinaSchool of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. ChinaCollege of Pharmacy, Guangdong Pharmaceutical University, Guangzhou, P.R. ChinaHealth Science College, Guangdong Pharmaceutical University, Guangzhou, P.R. ChinaSchool of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, P.R. ChinaAbstractContext Sinisan (SNS) has been used to treat psychosomatic diseases of the digestive system. But little is known about how SNS affects water immersion restraint stress (WIRS).Objective To study the effects of SNS on colonic tissue injury in the WIRS model.Materials and methods Forty-eight Kunming (KM) mice were randomized into 6 groups (n = 8): The control and WIRS groups receiving deionized water; the SNS low-dose (SL, 3.12 g/kg/d), SNS middle-dose (SM, 6.24 g/kg/d), SNS high-dose (SH, 12.48 g/kg/d), and diazepam (DZ, 5 mg/kg/d) groups; each with two daily administrations for 5 consecutive days. The 5 treatment groups were subjected to WIRS for 24 h on day 6. The effects of SNS on colon tissue injury caused by WIRS were assessed by changes in colon histology, inflammatory cytokines, brain-gut peptides, and tight junction (TJ) proteins levels. 16S rRNA gene sequencing was used to detect the regulation of the gut microbiota.Results SNS pretreatment significantly reduced TNF-α (0.75- to 0.81-fold), IL-6 (0.77-fold), and IFN-γ (0.69-fold) levels; and increased TJ proteins levels, such as ZO-1 (4.06- to 5.27-fold), claudin-1 (3.33- to 5.14-fold), and occludin (6.46- to 11.82-fold). However, there was no significant difference between the levels of substance P (SP) and vasoactive intestinal peptide (VIP) in the control and WIRS groups. SNS regulated the composition of gut microbiota in WIRS mice.Conclusion The positive effects of SNS on WIRS could provide a theoretical basis to treat stress-related gastrointestinal disorders.https://www.tandfonline.com/doi/10.1080/13880209.2023.2191643Inflammationintestinal floratight junctionintestinal barrierbrain-gut peptidemicrobiome-gut-brain axis |
| spellingShingle | Xiaoying Xu Huimei Hu Haizhou Zeng Boyi Li Qiuxiong Yin Yupeng Jiang Linquan Zang Changlin Zhao Guoqiang Qian Sinisan ameliorates colonic injury induced by water immersion restraint stress by enhancing intestinal barrier function and the gut microbiota structure Pharmaceutical Biology Inflammation intestinal flora tight junction intestinal barrier brain-gut peptide microbiome-gut-brain axis |
| title | Sinisan ameliorates colonic injury induced by water immersion restraint stress by enhancing intestinal barrier function and the gut microbiota structure |
| title_full | Sinisan ameliorates colonic injury induced by water immersion restraint stress by enhancing intestinal barrier function and the gut microbiota structure |
| title_fullStr | Sinisan ameliorates colonic injury induced by water immersion restraint stress by enhancing intestinal barrier function and the gut microbiota structure |
| title_full_unstemmed | Sinisan ameliorates colonic injury induced by water immersion restraint stress by enhancing intestinal barrier function and the gut microbiota structure |
| title_short | Sinisan ameliorates colonic injury induced by water immersion restraint stress by enhancing intestinal barrier function and the gut microbiota structure |
| title_sort | sinisan ameliorates colonic injury induced by water immersion restraint stress by enhancing intestinal barrier function and the gut microbiota structure |
| topic | Inflammation intestinal flora tight junction intestinal barrier brain-gut peptide microbiome-gut-brain axis |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2023.2191643 |
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