Antimicrobial resistance characteristics of Extended Spectrum Beta Lactamase (ESBL)- producing Escherichia coli from dairy farms in the Sleman district of Yogyakarta province, Indonesia
Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia coli (E. coli) infections are a global health challenge resulting from human contact with infected animals and contaminated farm environments. This study aims to identify antimicrobial resistance patterns of ESBL-producing E. col...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Chiang Mai University
2021-05-01
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| Series: | Veterinary Integrative Sciences |
| Online Access: | https://he02.tci-thaijo.org/index.php/vis/article/view/252653 |
| _version_ | 1797837756325429248 |
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| author | Kusnul Yuli Maulana Duangporn Pichpol Nur Rohmi Farhani Dyah Ayu Widiasih Fred Unger Veerasak Punyapornwithaya Tongkorn Meeyam |
| author_facet | Kusnul Yuli Maulana Duangporn Pichpol Nur Rohmi Farhani Dyah Ayu Widiasih Fred Unger Veerasak Punyapornwithaya Tongkorn Meeyam |
| author_sort | Kusnul Yuli Maulana |
| collection | DOAJ |
| description | Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia coli (E. coli) infections are a global health challenge resulting from human contact with infected animals and contaminated farm environments. This study aims to identify antimicrobial resistance patterns of ESBL-producing E. coli isolated from dairy farms in the Sleman District of Yogyakarta Province, Indonesia. Ninety-three dairy farms with a history of antibiotic use in the previous 6 months were identified. Samples were collected from 6 different sources (feces, milk, wastewater, animal drinking water, feed and rinses of workers’ hands) on each farm during August through November 2020. These samples were cultured with conventional microbiological methods for the isolation of ESBL-producing E. coli. ESBL-producing E. coli was identified in one or more of the sources in 54% (50/93) of the dairy farms sampled. Fecal samples were the most commonly positive (25%) while wastewater, animal drinking water feed, milk and hand rinses were positive at 16%, 10%, 5%, 4% and 3% respectively. Colonies from each positive sample were screened for antibiotic susceptibility test using the Vitek-2 system. Resistance to trimethoprim/sulfamethoxazole, tetracycline and gentamicin were found in 74%, 63% and 48% of the isolates, respectively. Multidrug resistant (MDR) was identified in 50% (63/127) of the isolates. In conclusion, ESBL-producing E. coli appears widespread in dairy farms using antibiotics and antimicrobial resistance among these bacteria is common in this study area. Further study of the risk of human transmission from contaminated cattle and their environments could benefit the national antimicrobial resistance strategic plans. |
| first_indexed | 2024-04-09T15:30:51Z |
| format | Article |
| id | doaj.art-1e90ccaea6694c89a77c504b058c5677 |
| institution | Directory Open Access Journal |
| issn | 2629-9968 |
| language | English |
| last_indexed | 2024-04-09T15:30:51Z |
| publishDate | 2021-05-01 |
| publisher | Faculty of Veterinary Medicine, Chiang Mai University |
| record_format | Article |
| series | Veterinary Integrative Sciences |
| spelling | doaj.art-1e90ccaea6694c89a77c504b058c56772023-04-28T08:22:51ZengFaculty of Veterinary Medicine, Chiang Mai UniversityVeterinary Integrative Sciences2629-99682021-05-0119352553510.12982/VIS.2021.041Antimicrobial resistance characteristics of Extended Spectrum Beta Lactamase (ESBL)- producing Escherichia coli from dairy farms in the Sleman district of Yogyakarta province, IndonesiaKusnul Yuli MaulanaDuangporn PichpolNur Rohmi FarhaniDyah Ayu WidiasihFred UngerVeerasak PunyapornwithayaTongkorn Meeyam Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia coli (E. coli) infections are a global health challenge resulting from human contact with infected animals and contaminated farm environments. This study aims to identify antimicrobial resistance patterns of ESBL-producing E. coli isolated from dairy farms in the Sleman District of Yogyakarta Province, Indonesia. Ninety-three dairy farms with a history of antibiotic use in the previous 6 months were identified. Samples were collected from 6 different sources (feces, milk, wastewater, animal drinking water, feed and rinses of workers’ hands) on each farm during August through November 2020. These samples were cultured with conventional microbiological methods for the isolation of ESBL-producing E. coli. ESBL-producing E. coli was identified in one or more of the sources in 54% (50/93) of the dairy farms sampled. Fecal samples were the most commonly positive (25%) while wastewater, animal drinking water feed, milk and hand rinses were positive at 16%, 10%, 5%, 4% and 3% respectively. Colonies from each positive sample were screened for antibiotic susceptibility test using the Vitek-2 system. Resistance to trimethoprim/sulfamethoxazole, tetracycline and gentamicin were found in 74%, 63% and 48% of the isolates, respectively. Multidrug resistant (MDR) was identified in 50% (63/127) of the isolates. In conclusion, ESBL-producing E. coli appears widespread in dairy farms using antibiotics and antimicrobial resistance among these bacteria is common in this study area. Further study of the risk of human transmission from contaminated cattle and their environments could benefit the national antimicrobial resistance strategic plans.https://he02.tci-thaijo.org/index.php/vis/article/view/252653 |
| spellingShingle | Kusnul Yuli Maulana Duangporn Pichpol Nur Rohmi Farhani Dyah Ayu Widiasih Fred Unger Veerasak Punyapornwithaya Tongkorn Meeyam Antimicrobial resistance characteristics of Extended Spectrum Beta Lactamase (ESBL)- producing Escherichia coli from dairy farms in the Sleman district of Yogyakarta province, Indonesia Veterinary Integrative Sciences |
| title | Antimicrobial resistance characteristics of Extended Spectrum Beta Lactamase (ESBL)- producing Escherichia coli from dairy farms in the Sleman district of Yogyakarta province, Indonesia |
| title_full | Antimicrobial resistance characteristics of Extended Spectrum Beta Lactamase (ESBL)- producing Escherichia coli from dairy farms in the Sleman district of Yogyakarta province, Indonesia |
| title_fullStr | Antimicrobial resistance characteristics of Extended Spectrum Beta Lactamase (ESBL)- producing Escherichia coli from dairy farms in the Sleman district of Yogyakarta province, Indonesia |
| title_full_unstemmed | Antimicrobial resistance characteristics of Extended Spectrum Beta Lactamase (ESBL)- producing Escherichia coli from dairy farms in the Sleman district of Yogyakarta province, Indonesia |
| title_short | Antimicrobial resistance characteristics of Extended Spectrum Beta Lactamase (ESBL)- producing Escherichia coli from dairy farms in the Sleman district of Yogyakarta province, Indonesia |
| title_sort | antimicrobial resistance characteristics of extended spectrum beta lactamase esbl producing escherichia coli from dairy farms in the sleman district of yogyakarta province indonesia |
| url | https://he02.tci-thaijo.org/index.php/vis/article/view/252653 |
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