Evaluation of Soil Streptomyces spp. for the Biological Control of Fusarium Wilt Disease and Growth Promotion in Tomato and Banana
Fusarium oxysporum f. sp. lycopersici (Fol) and Fusarium oxysporum f. sp. cubense (Foc), are the causal agent of Fusarium wilt disease of tomato and banana, respectively, and cause significant yield losses worldwide. A cost-effective measure, such as biological control agents, was used as an alterna...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2023-02-01
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| Series: | The Plant Pathology Journal |
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| Online Access: | http://ppjonline.org/upload/pdf/PPJ-OA-08-2022-0124.pdf |
| _version_ | 1811167159740530688 |
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| author | Praphat Kawicha Jariya Nitayaros Prakob Saman Sirikanya Thaporn Thanwanit Thanyasiriwat Khanitta Somtrakoon Kusavadee Sangdee Aphidech Sangdee |
| author_facet | Praphat Kawicha Jariya Nitayaros Prakob Saman Sirikanya Thaporn Thanwanit Thanyasiriwat Khanitta Somtrakoon Kusavadee Sangdee Aphidech Sangdee |
| author_sort | Praphat Kawicha |
| collection | DOAJ |
| description | Fusarium oxysporum f. sp. lycopersici (Fol) and Fusarium oxysporum f. sp. cubense (Foc), are the causal agent of Fusarium wilt disease of tomato and banana, respectively, and cause significant yield losses worldwide. A cost-effective measure, such as biological control agents, was used as an alternative method to control these pathogens. Therefore, in this study, six isolates of the Streptomyces-like colony were isolated from soils and their antagonistic activity against phytopathogenic fungi and plant growth-promoting (PGP) activity were assessed. The results showed that these isolates could inhibit the mycelial growth of Fol and Foc. Among them, isolate STRM304 showed the highest percentage of mycelial growth reduction and broad-spectrum antagonistic activity against all tested fungi. In the pot experiment study, the culture filtrate of isolates STRM103 and STRM104 significantly decreased disease severity and symptoms in Fol inoculated plants. Similarly, the culture filtrate of the STRM304 isolate significantly reduced the severity of the disease and symptoms of the disease in Foc inoculated plants. The PGP activity test presents PGP activities, such as indole acetic acid production, phosphate solubilization, starch hydrolysis, lignin hydrolysis, and cellulase activity. Interestingly, the application of the culture filtrate from all isolates increased the percentage of tomato seed germination and stimulated the growth of tomato plants and banana seedlings, increasing the elongation of the shoot and the root and shoot and root weight compared to the control treatment. Therefore, the isolate STRM103 and STRM104, and STRM304 could be used as biocontrol and PGP agents for tomato and banana, respectively, in sustainable agriculture. |
| first_indexed | 2024-04-10T16:04:29Z |
| format | Article |
| id | doaj.art-1e9af4cd2c364ac3b2aa52e46d30e645 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-04-10T16:04:29Z |
| publishDate | 2023-02-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-1e9af4cd2c364ac3b2aa52e46d30e6452023-02-10T05:32:58ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802023-02-0139110812210.5423/PPJ.OA.08.2022.01242374Evaluation of Soil Streptomyces spp. for the Biological Control of Fusarium Wilt Disease and Growth Promotion in Tomato and BananaPraphat Kawicha0Jariya Nitayaros1Prakob Saman2Sirikanya Thaporn3Thanwanit Thanyasiriwat4Khanitta Somtrakoon5Kusavadee Sangdee6Aphidech Sangdee7 Plant Pest and Biocontrol Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, Thailand Plant Pest and Biocontrol Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, Thailand Plant Pest and Biocontrol Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, Thailand Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand Plant Pest and Biocontrol Research Unit, Department of Agriculture and Resources, Faculty of Natural Resources and Agro-Industry, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, Thailand Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, Thailand Preclinical Group, Faculty of Medicine, Mahasarakham University, Muang District, Maha Sarakham 44000, Thailand Department of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, ThailandFusarium oxysporum f. sp. lycopersici (Fol) and Fusarium oxysporum f. sp. cubense (Foc), are the causal agent of Fusarium wilt disease of tomato and banana, respectively, and cause significant yield losses worldwide. A cost-effective measure, such as biological control agents, was used as an alternative method to control these pathogens. Therefore, in this study, six isolates of the Streptomyces-like colony were isolated from soils and their antagonistic activity against phytopathogenic fungi and plant growth-promoting (PGP) activity were assessed. The results showed that these isolates could inhibit the mycelial growth of Fol and Foc. Among them, isolate STRM304 showed the highest percentage of mycelial growth reduction and broad-spectrum antagonistic activity against all tested fungi. In the pot experiment study, the culture filtrate of isolates STRM103 and STRM104 significantly decreased disease severity and symptoms in Fol inoculated plants. Similarly, the culture filtrate of the STRM304 isolate significantly reduced the severity of the disease and symptoms of the disease in Foc inoculated plants. The PGP activity test presents PGP activities, such as indole acetic acid production, phosphate solubilization, starch hydrolysis, lignin hydrolysis, and cellulase activity. Interestingly, the application of the culture filtrate from all isolates increased the percentage of tomato seed germination and stimulated the growth of tomato plants and banana seedlings, increasing the elongation of the shoot and the root and shoot and root weight compared to the control treatment. Therefore, the isolate STRM103 and STRM104, and STRM304 could be used as biocontrol and PGP agents for tomato and banana, respectively, in sustainable agriculture.http://ppjonline.org/upload/pdf/PPJ-OA-08-2022-0124.pdfbiocontrolfusarium wiltplant growth promoting |
| spellingShingle | Praphat Kawicha Jariya Nitayaros Prakob Saman Sirikanya Thaporn Thanwanit Thanyasiriwat Khanitta Somtrakoon Kusavadee Sangdee Aphidech Sangdee Evaluation of Soil Streptomyces spp. for the Biological Control of Fusarium Wilt Disease and Growth Promotion in Tomato and Banana The Plant Pathology Journal biocontrol fusarium wilt plant growth promoting |
| title | Evaluation of Soil Streptomyces spp. for the Biological Control of Fusarium Wilt Disease and Growth Promotion in Tomato and Banana |
| title_full | Evaluation of Soil Streptomyces spp. for the Biological Control of Fusarium Wilt Disease and Growth Promotion in Tomato and Banana |
| title_fullStr | Evaluation of Soil Streptomyces spp. for the Biological Control of Fusarium Wilt Disease and Growth Promotion in Tomato and Banana |
| title_full_unstemmed | Evaluation of Soil Streptomyces spp. for the Biological Control of Fusarium Wilt Disease and Growth Promotion in Tomato and Banana |
| title_short | Evaluation of Soil Streptomyces spp. for the Biological Control of Fusarium Wilt Disease and Growth Promotion in Tomato and Banana |
| title_sort | evaluation of soil streptomyces spp for the biological control of fusarium wilt disease and growth promotion in tomato and banana |
| topic | biocontrol fusarium wilt plant growth promoting |
| url | http://ppjonline.org/upload/pdf/PPJ-OA-08-2022-0124.pdf |
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