Internal state affects local neuron function in an early sensory processing center to shape olfactory behavior in Drosophila larvae
Abstract Crawling insects, when starved, tend to have fewer head wavings and travel in straighter tracks in search of food. We used the Drosophila melanogaster larva to investigate whether this flexibility in the insect’s navigation strategy arises during early olfactory processing and, if so, how....
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-20147-1 |
| _version_ | 1828175472131833856 |
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| author | Seth R. Odell David Clark Nicholas Zito Roshni Jain Hui Gong Kendall Warnock Ricardo Carrion-Lopez Coral Maixner Lucia Prieto-Godino Dennis Mathew |
| author_facet | Seth R. Odell David Clark Nicholas Zito Roshni Jain Hui Gong Kendall Warnock Ricardo Carrion-Lopez Coral Maixner Lucia Prieto-Godino Dennis Mathew |
| author_sort | Seth R. Odell |
| collection | DOAJ |
| description | Abstract Crawling insects, when starved, tend to have fewer head wavings and travel in straighter tracks in search of food. We used the Drosophila melanogaster larva to investigate whether this flexibility in the insect’s navigation strategy arises during early olfactory processing and, if so, how. We demonstrate a critical role for Keystone-LN, an inhibitory local neuron in the antennal lobe, in implementing head-sweep behavior. Keystone-LN responds to odor stimuli, and its inhibitory output is required for a larva to successfully navigate attractive and aversive odor gradients. We show that insulin signaling in Keystone-LN likely mediates the starvation-dependent changes in head-sweep magnitude, shaping the larva’s odor-guided movement. Our findings demonstrate how flexibility in an insect’s navigation strategy can arise from context-dependent modulation of inhibitory neurons in an early sensory processing center. They raise new questions about modulating a circuit’s inhibitory output to implement changes in a goal-directed movement. |
| first_indexed | 2024-04-12T04:28:35Z |
| format | Article |
| id | doaj.art-1e9d2e0f4bf8497094536a9bae30a1fb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T04:28:35Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1e9d2e0f4bf8497094536a9bae30a1fb2022-12-22T03:48:00ZengNature PortfolioScientific Reports2045-23222022-09-0112111410.1038/s41598-022-20147-1Internal state affects local neuron function in an early sensory processing center to shape olfactory behavior in Drosophila larvaeSeth R. Odell0David Clark1Nicholas Zito2Roshni Jain3Hui Gong4Kendall Warnock5Ricardo Carrion-Lopez6Coral Maixner7Lucia Prieto-Godino8Dennis Mathew9Integrative Neuroscience Program, University of NevadaIntegrative Neuroscience Program, University of NevadaIntegrative Neuroscience Program, University of NevadaMolecular Biosciences Program, University of NevadaThe Francis Crick InstituteDepartment of Biology, University of NevadaNSF-REU (BioSoRo) Program, University of NevadaNSF-REU (BioSoRo) Program, University of NevadaThe Francis Crick InstituteIntegrative Neuroscience Program, University of NevadaAbstract Crawling insects, when starved, tend to have fewer head wavings and travel in straighter tracks in search of food. We used the Drosophila melanogaster larva to investigate whether this flexibility in the insect’s navigation strategy arises during early olfactory processing and, if so, how. We demonstrate a critical role for Keystone-LN, an inhibitory local neuron in the antennal lobe, in implementing head-sweep behavior. Keystone-LN responds to odor stimuli, and its inhibitory output is required for a larva to successfully navigate attractive and aversive odor gradients. We show that insulin signaling in Keystone-LN likely mediates the starvation-dependent changes in head-sweep magnitude, shaping the larva’s odor-guided movement. Our findings demonstrate how flexibility in an insect’s navigation strategy can arise from context-dependent modulation of inhibitory neurons in an early sensory processing center. They raise new questions about modulating a circuit’s inhibitory output to implement changes in a goal-directed movement.https://doi.org/10.1038/s41598-022-20147-1 |
| spellingShingle | Seth R. Odell David Clark Nicholas Zito Roshni Jain Hui Gong Kendall Warnock Ricardo Carrion-Lopez Coral Maixner Lucia Prieto-Godino Dennis Mathew Internal state affects local neuron function in an early sensory processing center to shape olfactory behavior in Drosophila larvae Scientific Reports |
| title | Internal state affects local neuron function in an early sensory processing center to shape olfactory behavior in Drosophila larvae |
| title_full | Internal state affects local neuron function in an early sensory processing center to shape olfactory behavior in Drosophila larvae |
| title_fullStr | Internal state affects local neuron function in an early sensory processing center to shape olfactory behavior in Drosophila larvae |
| title_full_unstemmed | Internal state affects local neuron function in an early sensory processing center to shape olfactory behavior in Drosophila larvae |
| title_short | Internal state affects local neuron function in an early sensory processing center to shape olfactory behavior in Drosophila larvae |
| title_sort | internal state affects local neuron function in an early sensory processing center to shape olfactory behavior in drosophila larvae |
| url | https://doi.org/10.1038/s41598-022-20147-1 |
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