The combined fixed-dose antituberculous drugs alter some reproductive functions with oxidative stress involvement in wistar rats
The reproductive toxicity of combined fixed-dose first-line antituberculosis (CFDAT) regimen was assessed in rats. Thirty-two (32) Wistar rats weighing 168.1 ± 8.0 g were divided into four groups of eight rats per group. Two groups of male and female rats were administered oral distilled water (1.6 ...
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| Format: | Article |
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Elsevier
2016-01-01
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| Series: | Toxicology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221475001630049X |
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| author | O. Awodele, B.Pharm M.Sc MPH PhD D.Sc FPCPharm FASI A.A. Momoh N.A. Awolola O.E. Kale W.O. Okunowo |
| author_facet | O. Awodele, B.Pharm M.Sc MPH PhD D.Sc FPCPharm FASI A.A. Momoh N.A. Awolola O.E. Kale W.O. Okunowo |
| author_sort | O. Awodele, B.Pharm M.Sc MPH PhD D.Sc FPCPharm FASI |
| collection | DOAJ |
| description | The reproductive toxicity of combined fixed-dose first-line antituberculosis (CFDAT) regimen was assessed in rats. Thirty-two (32) Wistar rats weighing 168.1 ± 8.0 g were divided into four groups of eight rats per group. Two groups of male and female rats were administered oral distilled water (1.6 ml) and CFDAT drugs containing rifampicin, isoniazid, pyrazinamide and ethambutol (RIPE, 92.5 mg/m2 per body surface area) respectively for forty-five days. Serum follicle stimulating hormone, luteinizing and testosterone were reduced significantly (p < 0.05) in the treated male rats. Similarly, sperm count levels were decreased by 27.3% when compared with control. RIPE elevated serum oestrogen (p < 0.05), progesterone (p < 0.05) as well as prolactin (p > 0.05) levels in the treated females. In addition, RIPE reduced (p < 0.05) total proteins levels and increased (p < 0.05, 53%) catalase levels in male but not female animals. Superoxide dismutase, glutathione-S-transferase, glutathione peroxidase, reduced glutathione levels as well as lipid peroxidation were unaltered in all rats respectively. Histopathological studies revealed congested peritesticular vessels and no changes in the ovary when compared with control. Overall, our results demonstrate reproductive toxicity potentials of RIPE in the rat, thus, suggesting that these reproductive parameters be monitored during antituberculous chemotherapy. Keywords: Fixed dose combined antituberculous drugs, Sub-chronic study, Reproductive toxicity, Rats |
| first_indexed | 2024-12-11T02:36:33Z |
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| id | doaj.art-1e9f50c4eb3344f7a6ba04161e69ca94 |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-12-11T02:36:33Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-1e9f50c4eb3344f7a6ba04161e69ca942022-12-22T01:23:43ZengElsevierToxicology Reports2214-75002016-01-013620627The combined fixed-dose antituberculous drugs alter some reproductive functions with oxidative stress involvement in wistar ratsO. Awodele, B.Pharm M.Sc MPH PhD D.Sc FPCPharm FASI0A.A. Momoh1N.A. Awolola2O.E. Kale3W.O. Okunowo4Department of Pharmacology, Therapeutics and Toxicology, College of Medicine, PMB 12003, Idi-Araba Campus, University of Lagos, Nigeria; Corresponding author at: Consultant Toxicologist, Toxicology Unit, Department of Pharmacology, Therapeutics & Toxicology, College of Medicine, University of Lagos, Lagos−Nigeria.Department of Pharmacology, Therapeutics and Toxicology, College of Medicine, PMB 12003, Idi-Araba Campus, University of Lagos, NigeriaDepartment of Anatomic and Molecular Pathology, College of Medicine, Idi-Araba Campus, University of Lagos, NigeriaDepartment of Pharmacology, Therapeutics and Toxicology, College of Medicine, PMB 12003, Idi-Araba Campus, University of Lagos, Nigeria; Department of Pharmacology, Benjamin Carson (Snr.) School of Medicine, Babcock University, Ilisan Remo, Ogun Nigeria, NigeriaDepartment of Biochemistry, College of Medicine University of Lagos, PMB 12003, Lagos, NigeriaThe reproductive toxicity of combined fixed-dose first-line antituberculosis (CFDAT) regimen was assessed in rats. Thirty-two (32) Wistar rats weighing 168.1 ± 8.0 g were divided into four groups of eight rats per group. Two groups of male and female rats were administered oral distilled water (1.6 ml) and CFDAT drugs containing rifampicin, isoniazid, pyrazinamide and ethambutol (RIPE, 92.5 mg/m2 per body surface area) respectively for forty-five days. Serum follicle stimulating hormone, luteinizing and testosterone were reduced significantly (p < 0.05) in the treated male rats. Similarly, sperm count levels were decreased by 27.3% when compared with control. RIPE elevated serum oestrogen (p < 0.05), progesterone (p < 0.05) as well as prolactin (p > 0.05) levels in the treated females. In addition, RIPE reduced (p < 0.05) total proteins levels and increased (p < 0.05, 53%) catalase levels in male but not female animals. Superoxide dismutase, glutathione-S-transferase, glutathione peroxidase, reduced glutathione levels as well as lipid peroxidation were unaltered in all rats respectively. Histopathological studies revealed congested peritesticular vessels and no changes in the ovary when compared with control. Overall, our results demonstrate reproductive toxicity potentials of RIPE in the rat, thus, suggesting that these reproductive parameters be monitored during antituberculous chemotherapy. Keywords: Fixed dose combined antituberculous drugs, Sub-chronic study, Reproductive toxicity, Ratshttp://www.sciencedirect.com/science/article/pii/S221475001630049X |
| spellingShingle | O. Awodele, B.Pharm M.Sc MPH PhD D.Sc FPCPharm FASI A.A. Momoh N.A. Awolola O.E. Kale W.O. Okunowo The combined fixed-dose antituberculous drugs alter some reproductive functions with oxidative stress involvement in wistar rats Toxicology Reports |
| title | The combined fixed-dose antituberculous drugs alter some reproductive functions with oxidative stress involvement in wistar rats |
| title_full | The combined fixed-dose antituberculous drugs alter some reproductive functions with oxidative stress involvement in wistar rats |
| title_fullStr | The combined fixed-dose antituberculous drugs alter some reproductive functions with oxidative stress involvement in wistar rats |
| title_full_unstemmed | The combined fixed-dose antituberculous drugs alter some reproductive functions with oxidative stress involvement in wistar rats |
| title_short | The combined fixed-dose antituberculous drugs alter some reproductive functions with oxidative stress involvement in wistar rats |
| title_sort | combined fixed dose antituberculous drugs alter some reproductive functions with oxidative stress involvement in wistar rats |
| url | http://www.sciencedirect.com/science/article/pii/S221475001630049X |
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