IL4I1 in M2-like macrophage promotes glioma progression and is a promising target for immunotherapy
BackgroundGlioma is the prevailing malignant intracranial tumor, characterized by an abundance of macrophages. Specifically, the infiltrating macrophages often display the M2 subtype and are known as tumor-associated macrophages (TAMs). They have a critical role in promoting the oncogenic properties...
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1338244/full |
| _version_ | 1797365561949159424 |
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| author | Feng Ye Lichong Wang Yuanyou Li Chengyuan Dong Liangxue Zhou Jianguo Xu |
| author_facet | Feng Ye Lichong Wang Yuanyou Li Chengyuan Dong Liangxue Zhou Jianguo Xu |
| author_sort | Feng Ye |
| collection | DOAJ |
| description | BackgroundGlioma is the prevailing malignant intracranial tumor, characterized by an abundance of macrophages. Specifically, the infiltrating macrophages often display the M2 subtype and are known as tumor-associated macrophages (TAMs). They have a critical role in promoting the oncogenic properties of tumor cells. Interleukin-4-induced-1 (IL4I1) functions as an L-phenylalanine oxidase, playing a key part in regulating immune responses and the progression of various tumors. However, there is limited understanding of the IL4I1-mediated cross-talk function between TAMs and glioma cell in the glioma microenvironment.MethodsTCGA, GTEx, and HPA databases were applied to assess the IL4I1 expression, clinical characteristics, and prognostic value of pan-cancer. The link between IL4I1 levels and the prognosis, methylation, and immune checkpoints (ICs) in gliomas were explored through Kaplan–Meier curve, Cox regression, and Spearman correlation analyses. The IL4I1 levels and their distribution were investigated by single-cell analysis and the TIMER 2 database. Additionally, validation of IL4I1 expression was performed by WB, RT-qPCR, IHC, and IF. Co-culture models between glioma cells and M2-like macrophages were used to explore the IL4I1-mediated effects on tumor growth, invasion, and migration of glioma cells. Moreover, the function of IL4I1 on macrophage polarization was evaluated by ELISA, RT-qPCR, WB, and siRNA transfection.ResultsBoth transcriptome and protein levels of IL4I1 were increased obviously in various tumor types, and correlated with a dismal prognosis. Specifically, IL4I1 was implicated in aggressive progression and a dismal prognosis for patients with glioma. A negative association was noticed between the glioma grade and DNA promoter methylation of IL4I1. Enrichment analyses in glioma patients suggested that IL4I1 was linked to cytokine and immune responses, and was positively correlated with ICs. Single-cell analysis, molecular experiments, and in vitro assays showed that IL4I1 was significantly expressed in TAMs. Importantly, co-culture models proved that IL4I1 significantly promoted the invasion and migration of glioma cells, and induced the polarization of M2-like macrophages.ConclusionIL4I1 could be a promising immunotherapy target for selective modulation of TAMs and stands as a novel macrophage-related prognostic biomarker in glioma. |
| first_indexed | 2024-03-08T16:51:42Z |
| format | Article |
| id | doaj.art-1e9f6e5f2f8c465fa156a6aa6805c545 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-08T16:51:42Z |
| publishDate | 2024-01-01 |
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| series | Frontiers in Immunology |
| spelling | doaj.art-1e9f6e5f2f8c465fa156a6aa6805c5452024-01-05T04:20:58ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-01-011410.3389/fimmu.2023.13382441338244IL4I1 in M2-like macrophage promotes glioma progression and is a promising target for immunotherapyFeng YeLichong WangYuanyou LiChengyuan DongLiangxue ZhouJianguo XuBackgroundGlioma is the prevailing malignant intracranial tumor, characterized by an abundance of macrophages. Specifically, the infiltrating macrophages often display the M2 subtype and are known as tumor-associated macrophages (TAMs). They have a critical role in promoting the oncogenic properties of tumor cells. Interleukin-4-induced-1 (IL4I1) functions as an L-phenylalanine oxidase, playing a key part in regulating immune responses and the progression of various tumors. However, there is limited understanding of the IL4I1-mediated cross-talk function between TAMs and glioma cell in the glioma microenvironment.MethodsTCGA, GTEx, and HPA databases were applied to assess the IL4I1 expression, clinical characteristics, and prognostic value of pan-cancer. The link between IL4I1 levels and the prognosis, methylation, and immune checkpoints (ICs) in gliomas were explored through Kaplan–Meier curve, Cox regression, and Spearman correlation analyses. The IL4I1 levels and their distribution were investigated by single-cell analysis and the TIMER 2 database. Additionally, validation of IL4I1 expression was performed by WB, RT-qPCR, IHC, and IF. Co-culture models between glioma cells and M2-like macrophages were used to explore the IL4I1-mediated effects on tumor growth, invasion, and migration of glioma cells. Moreover, the function of IL4I1 on macrophage polarization was evaluated by ELISA, RT-qPCR, WB, and siRNA transfection.ResultsBoth transcriptome and protein levels of IL4I1 were increased obviously in various tumor types, and correlated with a dismal prognosis. Specifically, IL4I1 was implicated in aggressive progression and a dismal prognosis for patients with glioma. A negative association was noticed between the glioma grade and DNA promoter methylation of IL4I1. Enrichment analyses in glioma patients suggested that IL4I1 was linked to cytokine and immune responses, and was positively correlated with ICs. Single-cell analysis, molecular experiments, and in vitro assays showed that IL4I1 was significantly expressed in TAMs. Importantly, co-culture models proved that IL4I1 significantly promoted the invasion and migration of glioma cells, and induced the polarization of M2-like macrophages.ConclusionIL4I1 could be a promising immunotherapy target for selective modulation of TAMs and stands as a novel macrophage-related prognostic biomarker in glioma.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1338244/fullIL4I1gliomatumor microenvironmentimmunotherapy targetM2-like macrophageTAM |
| spellingShingle | Feng Ye Lichong Wang Yuanyou Li Chengyuan Dong Liangxue Zhou Jianguo Xu IL4I1 in M2-like macrophage promotes glioma progression and is a promising target for immunotherapy Frontiers in Immunology IL4I1 glioma tumor microenvironment immunotherapy target M2-like macrophage TAM |
| title | IL4I1 in M2-like macrophage promotes glioma progression and is a promising target for immunotherapy |
| title_full | IL4I1 in M2-like macrophage promotes glioma progression and is a promising target for immunotherapy |
| title_fullStr | IL4I1 in M2-like macrophage promotes glioma progression and is a promising target for immunotherapy |
| title_full_unstemmed | IL4I1 in M2-like macrophage promotes glioma progression and is a promising target for immunotherapy |
| title_short | IL4I1 in M2-like macrophage promotes glioma progression and is a promising target for immunotherapy |
| title_sort | il4i1 in m2 like macrophage promotes glioma progression and is a promising target for immunotherapy |
| topic | IL4I1 glioma tumor microenvironment immunotherapy target M2-like macrophage TAM |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1338244/full |
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