piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition
Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Genome Editing |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgeed.2021.786893/full |
| _version_ | 1819038639574745088 |
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| author | Jonas Holst Wolff Jakob Haldrup Emil Aagaard Thomsen Sofie Andersen Jacob Giehm Mikkelsen |
| author_facet | Jonas Holst Wolff Jakob Haldrup Emil Aagaard Thomsen Sofie Andersen Jacob Giehm Mikkelsen |
| author_sort | Jonas Holst Wolff |
| collection | DOAJ |
| description | Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines. |
| first_indexed | 2024-12-21T08:40:31Z |
| format | Article |
| id | doaj.art-1e9f9970af5f4f1bbb8f20886290ab8a |
| institution | Directory Open Access Journal |
| issn | 2673-3439 |
| language | English |
| last_indexed | 2024-12-21T08:40:31Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genome Editing |
| spelling | doaj.art-1e9f9970af5f4f1bbb8f20886290ab8a2022-12-21T19:09:57ZengFrontiers Media S.A.Frontiers in Genome Editing2673-34392021-11-01310.3389/fgeed.2021.786893786893piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA TranspositionJonas Holst WolffJakob HaldrupEmil Aagaard ThomsenSofie AndersenJacob Giehm MikkelsenPrime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines.https://www.frontiersin.org/articles/10.3389/fgeed.2021.786893/fullprime editingCRISPRprime editing guide RNApegRNApiggyBacDNA transposition |
| spellingShingle | Jonas Holst Wolff Jakob Haldrup Emil Aagaard Thomsen Sofie Andersen Jacob Giehm Mikkelsen piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition Frontiers in Genome Editing prime editing CRISPR prime editing guide RNA pegRNA piggyBac DNA transposition |
| title | piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition |
| title_full | piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition |
| title_fullStr | piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition |
| title_full_unstemmed | piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition |
| title_short | piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition |
| title_sort | piggyprime high efficacy prime editing in human cells using piggybac based dna transposition |
| topic | prime editing CRISPR prime editing guide RNA pegRNA piggyBac DNA transposition |
| url | https://www.frontiersin.org/articles/10.3389/fgeed.2021.786893/full |
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