piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition

piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition

Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition...

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Bibliographic Details
Main Authors:	Jonas Holst Wolff, Jakob Haldrup, Emil Aagaard Thomsen, Sofie Andersen, Jacob Giehm Mikkelsen
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2021-11-01
Series:	Frontiers in Genome Editing
Subjects:	prime editing CRISPR prime editing guide RNA pegRNA piggyBac DNA transposition
Online Access:	https://www.frontiersin.org/articles/10.3389/fgeed.2021.786893/full

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