Rapid Diagnosis of <i>Pseudomonas aeruginosa</i> in Wounds with Point-Of-Care Fluorescence Imaing
<i>Pseudomonas aeruginosa</i> (PA) is a common bacterial pathogen in chronic wounds known for its propensity to form biofilms and evade conventional treatment methods. Early detection of PA in wounds is critical to the mitigation of more severe wound outcomes. Point-of-care bacterial flu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/11/2/280 |
| _version_ | 1797411272221786112 |
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| author | Rose Raizman William Little Allie Clinton Smith |
| author_facet | Rose Raizman William Little Allie Clinton Smith |
| author_sort | Rose Raizman |
| collection | DOAJ |
| description | <i>Pseudomonas aeruginosa</i> (PA) is a common bacterial pathogen in chronic wounds known for its propensity to form biofilms and evade conventional treatment methods. Early detection of PA in wounds is critical to the mitigation of more severe wound outcomes. Point-of-care bacterial fluorescence imaging illuminates wounds with safe, violet light, triggering the production of cyan fluorescence from PA. A prospective single blind clinical study was conducted to determine the positive predictive value (PPV) of cyan fluorescence for the detection of PA in wounds. Bacterial fluorescence using the MolecuLight <i>i:X</i> imaging device revealed cyan fluorescence signal in 28 chronic wounds, including venous leg ulcers, surgical wounds, diabetic foot ulcers and other wound types. To correlate the cyan signal to the presence of PA, wound regions positive for cyan fluorescence were sampled via curettage. A semi-quantitative culture analysis of curettage samples confirmed the presence of PA in 26/28 wounds, resulting in a PPV of 92.9%. The bacterial load of PA from cyan-positive regions ranged from light to heavy. Less than 20% of wounds that were positive for PA exhibited the classic symptoms of PA infection. These findings suggest that cyan detected on fluorescence images can be used to reliably predict bacteria, specifically PA at the point-of-care. |
| first_indexed | 2024-03-09T04:42:41Z |
| format | Article |
| id | doaj.art-1ea0d2b34363451d9d8eb9e4095bd32a |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T04:42:41Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-1ea0d2b34363451d9d8eb9e4095bd32a2023-12-03T13:18:41ZengMDPI AGDiagnostics2075-44182021-02-0111228010.3390/diagnostics11020280Rapid Diagnosis of <i>Pseudomonas aeruginosa</i> in Wounds with Point-Of-Care Fluorescence ImaingRose Raizman0William Little1Allie Clinton Smith2Department of Professional Practice, Scarborough Health Network, Lawrence S. Bloomberg Faculty of Nursing, University of Toronto, Toronto, ON M1E 4B9, CanadaDepartment of Honors Studies, Texas Tech University, Lubbock, TX 79409, USADepartment of Honors Studies, Texas Tech University, Lubbock, TX 79409, USA<i>Pseudomonas aeruginosa</i> (PA) is a common bacterial pathogen in chronic wounds known for its propensity to form biofilms and evade conventional treatment methods. Early detection of PA in wounds is critical to the mitigation of more severe wound outcomes. Point-of-care bacterial fluorescence imaging illuminates wounds with safe, violet light, triggering the production of cyan fluorescence from PA. A prospective single blind clinical study was conducted to determine the positive predictive value (PPV) of cyan fluorescence for the detection of PA in wounds. Bacterial fluorescence using the MolecuLight <i>i:X</i> imaging device revealed cyan fluorescence signal in 28 chronic wounds, including venous leg ulcers, surgical wounds, diabetic foot ulcers and other wound types. To correlate the cyan signal to the presence of PA, wound regions positive for cyan fluorescence were sampled via curettage. A semi-quantitative culture analysis of curettage samples confirmed the presence of PA in 26/28 wounds, resulting in a PPV of 92.9%. The bacterial load of PA from cyan-positive regions ranged from light to heavy. Less than 20% of wounds that were positive for PA exhibited the classic symptoms of PA infection. These findings suggest that cyan detected on fluorescence images can be used to reliably predict bacteria, specifically PA at the point-of-care.https://www.mdpi.com/2075-4418/11/2/280bacteriafluorescence imaging device<i>Pseudomonas aeruginosa</i>chronic wounds |
| spellingShingle | Rose Raizman William Little Allie Clinton Smith Rapid Diagnosis of <i>Pseudomonas aeruginosa</i> in Wounds with Point-Of-Care Fluorescence Imaing Diagnostics bacteria fluorescence imaging device <i>Pseudomonas aeruginosa</i> chronic wounds |
| title | Rapid Diagnosis of <i>Pseudomonas aeruginosa</i> in Wounds with Point-Of-Care Fluorescence Imaing |
| title_full | Rapid Diagnosis of <i>Pseudomonas aeruginosa</i> in Wounds with Point-Of-Care Fluorescence Imaing |
| title_fullStr | Rapid Diagnosis of <i>Pseudomonas aeruginosa</i> in Wounds with Point-Of-Care Fluorescence Imaing |
| title_full_unstemmed | Rapid Diagnosis of <i>Pseudomonas aeruginosa</i> in Wounds with Point-Of-Care Fluorescence Imaing |
| title_short | Rapid Diagnosis of <i>Pseudomonas aeruginosa</i> in Wounds with Point-Of-Care Fluorescence Imaing |
| title_sort | rapid diagnosis of i pseudomonas aeruginosa i in wounds with point of care fluorescence imaing |
| topic | bacteria fluorescence imaging device <i>Pseudomonas aeruginosa</i> chronic wounds |
| url | https://www.mdpi.com/2075-4418/11/2/280 |
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