A proposal to improve gene expression analysis
Abstract Gene expression profiling is commonly used to define molecular subtypes in cancers and gene sets can be identified as prognostic indicators or as potential biomarkers. Reverse transcription‐quantitative real‐time polymerase chain reaction is commonly used to measure relative changes in gene...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-03-01
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| Series: | Clinical and Translational Discovery |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ctd2.26 |
| _version_ | 1828057311968493568 |
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| author | Mohammad Amin Kerachian Marjan Azghandi Jean Paul Thiery |
| author_facet | Mohammad Amin Kerachian Marjan Azghandi Jean Paul Thiery |
| author_sort | Mohammad Amin Kerachian |
| collection | DOAJ |
| description | Abstract Gene expression profiling is commonly used to define molecular subtypes in cancers and gene sets can be identified as prognostic indicators or as potential biomarkers. Reverse transcription‐quantitative real‐time polymerase chain reaction is commonly used to measure relative changes in gene transcript messenger RNA levels. However, given target gene expression levels are inconstantly reported in the literature. The variable quantification of the gene expression level could be affected by intra/intergenic noncoding RNAs. To overcome this problem, we suggest the analysis be performed using at least two sets of primers, which are designed in two different regions of the target gene. |
| first_indexed | 2024-04-10T21:09:32Z |
| format | Article |
| id | doaj.art-1ea15466644c43f084943fb7145138f2 |
| institution | Directory Open Access Journal |
| issn | 2768-0622 |
| language | English |
| last_indexed | 2024-04-10T21:09:32Z |
| publishDate | 2022-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Translational Discovery |
| spelling | doaj.art-1ea15466644c43f084943fb7145138f22023-01-21T04:01:41ZengWileyClinical and Translational Discovery2768-06222022-03-0121n/an/a10.1002/ctd2.26A proposal to improve gene expression analysisMohammad Amin Kerachian0Marjan Azghandi1Jean Paul Thiery2Medical Genetics Research Center Mashhad University of Medical Sciences Mashhad IranCancer Genetics Research Unit Reza Radiotherapy and Oncology Center Mashhad IranBioland Laboratory Guangzhou Regenerative Medicine and Health Guangdong Laboratory Guangzhou ChinaAbstract Gene expression profiling is commonly used to define molecular subtypes in cancers and gene sets can be identified as prognostic indicators or as potential biomarkers. Reverse transcription‐quantitative real‐time polymerase chain reaction is commonly used to measure relative changes in gene transcript messenger RNA levels. However, given target gene expression levels are inconstantly reported in the literature. The variable quantification of the gene expression level could be affected by intra/intergenic noncoding RNAs. To overcome this problem, we suggest the analysis be performed using at least two sets of primers, which are designed in two different regions of the target gene.https://doi.org/10.1002/ctd2.26exon‐exon junctiongene expressionprimer designRT‐qPCR |
| spellingShingle | Mohammad Amin Kerachian Marjan Azghandi Jean Paul Thiery A proposal to improve gene expression analysis Clinical and Translational Discovery exon‐exon junction gene expression primer design RT‐qPCR |
| title | A proposal to improve gene expression analysis |
| title_full | A proposal to improve gene expression analysis |
| title_fullStr | A proposal to improve gene expression analysis |
| title_full_unstemmed | A proposal to improve gene expression analysis |
| title_short | A proposal to improve gene expression analysis |
| title_sort | proposal to improve gene expression analysis |
| topic | exon‐exon junction gene expression primer design RT‐qPCR |
| url | https://doi.org/10.1002/ctd2.26 |
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