Depletion and reconstitution of macrophages in mice with vesicular stomatitis virus infection
Summary: Acting as the first line of host defense, macrophages play a central role in antiviral response. Here, we present a protocol for depletion and reconstitution of macrophages in mice with vesicular stomatitis virus (VSV) infection. We describe steps for induction and isolation of peritoneal m...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723002861 |
| _version_ | 1797824491184717824 |
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| author | Yingchun Wang Lifen Gao |
| author_facet | Yingchun Wang Lifen Gao |
| author_sort | Yingchun Wang |
| collection | DOAJ |
| description | Summary: Acting as the first line of host defense, macrophages play a central role in antiviral response. Here, we present a protocol for depletion and reconstitution of macrophages in mice with vesicular stomatitis virus (VSV) infection. We describe steps for induction and isolation of peritoneal macrophages from CD45.2+ donor mice, macrophage depletion in CD45.1+ recipient mice, adoptive transfer of CD45.2+ macrophages to CD45.1+ recipient mice, and VSV infection. This protocol highlights the role of exogenous macrophages in antiviral response in vivo.For complete details on the use and execution of this profile, please refer to Wang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-13T10:39:46Z |
| format | Article |
| id | doaj.art-1ea4986c23394d98927ace938a1a7975 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-13T10:39:46Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-1ea4986c23394d98927ace938a1a79752023-05-18T04:40:16ZengElsevierSTAR Protocols2666-16672023-06-0142102319Depletion and reconstitution of macrophages in mice with vesicular stomatitis virus infectionYingchun Wang0Lifen Gao1Key Laboratory for Experimental Teratology of Ministry of Education, Shandong Key Laboratory of Infection and Immunity, and Department of Immunology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, P.R. ChinaKey Laboratory for Experimental Teratology of Ministry of Education, Shandong Key Laboratory of Infection and Immunity, and Department of Immunology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, P.R. China; Corresponding authorSummary: Acting as the first line of host defense, macrophages play a central role in antiviral response. Here, we present a protocol for depletion and reconstitution of macrophages in mice with vesicular stomatitis virus (VSV) infection. We describe steps for induction and isolation of peritoneal macrophages from CD45.2+ donor mice, macrophage depletion in CD45.1+ recipient mice, adoptive transfer of CD45.2+ macrophages to CD45.1+ recipient mice, and VSV infection. This protocol highlights the role of exogenous macrophages in antiviral response in vivo.For complete details on the use and execution of this profile, please refer to Wang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723002861Cell isolationImmunology |
| spellingShingle | Yingchun Wang Lifen Gao Depletion and reconstitution of macrophages in mice with vesicular stomatitis virus infection STAR Protocols Cell isolation Immunology |
| title | Depletion and reconstitution of macrophages in mice with vesicular stomatitis virus infection |
| title_full | Depletion and reconstitution of macrophages in mice with vesicular stomatitis virus infection |
| title_fullStr | Depletion and reconstitution of macrophages in mice with vesicular stomatitis virus infection |
| title_full_unstemmed | Depletion and reconstitution of macrophages in mice with vesicular stomatitis virus infection |
| title_short | Depletion and reconstitution of macrophages in mice with vesicular stomatitis virus infection |
| title_sort | depletion and reconstitution of macrophages in mice with vesicular stomatitis virus infection |
| topic | Cell isolation Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723002861 |
| work_keys_str_mv | AT yingchunwang depletionandreconstitutionofmacrophagesinmicewithvesicularstomatitisvirusinfection AT lifengao depletionandreconstitutionofmacrophagesinmicewithvesicularstomatitisvirusinfection |