Micronutrient specific SSR marker based diversity analysis for developing short duration wheat (Triticum aestivum)

Malnutrition leads to high mortality especially in developing countries. Present study comprised 11 high yielding wheat (Triticum aestivum L.) genotypes sown during rabi 2014-15 and 2015-16 and crosses attempted during 2014-15 and sown at Research Field of CCS Haryana Agricultural University, Hisar...

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Main Authors: DIVYA PHOUGAT, S K SETHI
Format: Article
Language:English
Published: Indian Council of Agricultural Research 2020-07-01
Series:The Indian Journal of Agricultural Sciences
Subjects:
Online Access:https://epubs.icar.org.in/index.php/IJAgS/article/view/102146
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author DIVYA PHOUGAT
S K SETHI
author_facet DIVYA PHOUGAT
S K SETHI
author_sort DIVYA PHOUGAT
collection DOAJ
description Malnutrition leads to high mortality especially in developing countries. Present study comprised 11 high yielding wheat (Triticum aestivum L.) genotypes sown during rabi 2014-15 and 2015-16 and crosses attempted during 2014-15 and sown at Research Field of CCS Haryana Agricultural University, Hisar, Haryana. Forty nine microsatellite markers linked with identified QTLs for micronutrient were used for the genetic diversity assessment among bread wheat parents their F1s. Out of 49, 38 SSRs were found polymorphic which amplified a total of 76 alleles with an average of 2.00 alleles per locus. The range of amplified PCR products ranged from 100 to 480 bp. Estimates of similarity coefficient among parents and F1s suggest enough divergence and averaged to 0.71. Enough genetic variability was observed for all the 14 agronomic traits in all genotypes. Selection of genotypes from different clusters helps in making proper choice of breeding programme. In the present study, the information generated about genetic diversity will be useful for selection of parents for breeding including gene mapping and ultimately for marker assisted selection (MAS) for micronutrient content improvement worldwide.
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spelling doaj.art-1ea56d49df384abbb68ac910dbbc52282023-02-14T09:10:07ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192020-07-0190410.56093/ijas.v90i4.102146Micronutrient specific SSR marker based diversity analysis for developing short duration wheat (Triticum aestivum)DIVYA PHOUGAT0S K SETHI1Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, IndiaChaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India Malnutrition leads to high mortality especially in developing countries. Present study comprised 11 high yielding wheat (Triticum aestivum L.) genotypes sown during rabi 2014-15 and 2015-16 and crosses attempted during 2014-15 and sown at Research Field of CCS Haryana Agricultural University, Hisar, Haryana. Forty nine microsatellite markers linked with identified QTLs for micronutrient were used for the genetic diversity assessment among bread wheat parents their F1s. Out of 49, 38 SSRs were found polymorphic which amplified a total of 76 alleles with an average of 2.00 alleles per locus. The range of amplified PCR products ranged from 100 to 480 bp. Estimates of similarity coefficient among parents and F1s suggest enough divergence and averaged to 0.71. Enough genetic variability was observed for all the 14 agronomic traits in all genotypes. Selection of genotypes from different clusters helps in making proper choice of breeding programme. In the present study, the information generated about genetic diversity will be useful for selection of parents for breeding including gene mapping and ultimately for marker assisted selection (MAS) for micronutrient content improvement worldwide. https://epubs.icar.org.in/index.php/IJAgS/article/view/102146DiversityMalnutritionMicronutrientMicrosatellites markers
spellingShingle DIVYA PHOUGAT
S K SETHI
Micronutrient specific SSR marker based diversity analysis for developing short duration wheat (Triticum aestivum)
The Indian Journal of Agricultural Sciences
Diversity
Malnutrition
Micronutrient
Microsatellites markers
title Micronutrient specific SSR marker based diversity analysis for developing short duration wheat (Triticum aestivum)
title_full Micronutrient specific SSR marker based diversity analysis for developing short duration wheat (Triticum aestivum)
title_fullStr Micronutrient specific SSR marker based diversity analysis for developing short duration wheat (Triticum aestivum)
title_full_unstemmed Micronutrient specific SSR marker based diversity analysis for developing short duration wheat (Triticum aestivum)
title_short Micronutrient specific SSR marker based diversity analysis for developing short duration wheat (Triticum aestivum)
title_sort micronutrient specific ssr marker based diversity analysis for developing short duration wheat triticum aestivum
topic Diversity
Malnutrition
Micronutrient
Microsatellites markers
url https://epubs.icar.org.in/index.php/IJAgS/article/view/102146
work_keys_str_mv AT divyaphougat micronutrientspecificssrmarkerbaseddiversityanalysisfordevelopingshortdurationwheattriticumaestivum
AT sksethi micronutrientspecificssrmarkerbaseddiversityanalysisfordevelopingshortdurationwheattriticumaestivum