Regulatory Mechanism and Experimental Verification of Patchouli Alcohol on Gastric Cancer Cell Based on Network Pharmacology
BackgroundPogostemon cablin is a traditional Chinese medicine (TCM) that is frequently used to treat various gastrointestinal diseases. Patchouli alcohol (PA), a compound extracted from the Pogostemon cablin, has been shown to have anti-tumor efficacy in human colorectal cancer. However, the mechani...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2021.711984/full |
| _version_ | 1828961681000103936 |
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| author | Yanru Song Liang Chang Xiaoyuan Wang Bibo Tan Jianbo Li Jie Zhang Fengbin Zhang Lianmei Zhao Guangjie Liu Bingjie Huo |
| author_facet | Yanru Song Liang Chang Xiaoyuan Wang Bibo Tan Jianbo Li Jie Zhang Fengbin Zhang Lianmei Zhao Guangjie Liu Bingjie Huo |
| author_sort | Yanru Song |
| collection | DOAJ |
| description | BackgroundPogostemon cablin is a traditional Chinese medicine (TCM) that is frequently used to treat various gastrointestinal diseases. Patchouli alcohol (PA), a compound extracted from the Pogostemon cablin, has been shown to have anti-tumor efficacy in human colorectal cancer. However, the mechanism of PA’s anticancer effect on gastric cancer (GC) remains unknown.MethodsWe used the public database to obtain the potential targets of PA and genes related to GC. Bioinformatic analyses, such as the Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and protein-protein interactions (PPI), were used for analyzing the potential signal pathways and targets. Cell experiments were also conducted to further explain the impact and molecular mechanism of PA on GC, as well as to confirm the findings of network pharmacology.ResultsUsing network pharmacological analysis, 161 possible targets were identified for the treatment of GC. Network analysis and functional enrichment analysis show that PA produced a marked effect in the treatment of GC through multi-targets and multi-pathways, especially the MAPK and PI3K/AKT signal pathways. In addition, PA showed the inhibition of GC cell proliferation, migration and invasion in cell experiments. According to our findings, PA could also cause G0/G1 phase arrest and apoptosis in GC cells.ConclusionUsing network pharmacology, we aim to uncover the possible molecular mechanism of PA on GC treatment in this research. Cell experiments were also conducted to confirm the therapeutic effect of PA on GC. |
| first_indexed | 2024-12-14T09:50:02Z |
| format | Article |
| id | doaj.art-1ea5e02416e7442898394999671d2858 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-14T09:50:02Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-1ea5e02416e7442898394999671d28582022-12-21T23:07:32ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-09-011110.3389/fonc.2021.711984711984Regulatory Mechanism and Experimental Verification of Patchouli Alcohol on Gastric Cancer Cell Based on Network PharmacologyYanru Song0Liang Chang1Xiaoyuan Wang2Bibo Tan3Jianbo Li4Jie Zhang5Fengbin Zhang6Lianmei Zhao7Guangjie Liu8Bingjie Huo9Department of Traditional Chinese Medicine, The Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Basic Theory of Traditional Chinese Medicine of Basic Medical Science College, HeBei University of Chinese Medicine, Shijiazhuang, ChinaDepartment of Traditional Chinese Medicine, The Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of General Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Traditional Chinese Medicine, The Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Traditional Chinese Medicine, The Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Gastroenterology Pharmacology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaResearch Centre, The Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaDepartment of Traditional Chinese Medicine, The Fourth Hospital of Hebei Medical University, Shijiazhuang, ChinaBackgroundPogostemon cablin is a traditional Chinese medicine (TCM) that is frequently used to treat various gastrointestinal diseases. Patchouli alcohol (PA), a compound extracted from the Pogostemon cablin, has been shown to have anti-tumor efficacy in human colorectal cancer. However, the mechanism of PA’s anticancer effect on gastric cancer (GC) remains unknown.MethodsWe used the public database to obtain the potential targets of PA and genes related to GC. Bioinformatic analyses, such as the Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and protein-protein interactions (PPI), were used for analyzing the potential signal pathways and targets. Cell experiments were also conducted to further explain the impact and molecular mechanism of PA on GC, as well as to confirm the findings of network pharmacology.ResultsUsing network pharmacological analysis, 161 possible targets were identified for the treatment of GC. Network analysis and functional enrichment analysis show that PA produced a marked effect in the treatment of GC through multi-targets and multi-pathways, especially the MAPK and PI3K/AKT signal pathways. In addition, PA showed the inhibition of GC cell proliferation, migration and invasion in cell experiments. According to our findings, PA could also cause G0/G1 phase arrest and apoptosis in GC cells.ConclusionUsing network pharmacology, we aim to uncover the possible molecular mechanism of PA on GC treatment in this research. Cell experiments were also conducted to confirm the therapeutic effect of PA on GC.https://www.frontiersin.org/articles/10.3389/fonc.2021.711984/fullgastric cancernetwork pharmacologyproliferationapoptosisPatchouli alcohol |
| spellingShingle | Yanru Song Liang Chang Xiaoyuan Wang Bibo Tan Jianbo Li Jie Zhang Fengbin Zhang Lianmei Zhao Guangjie Liu Bingjie Huo Regulatory Mechanism and Experimental Verification of Patchouli Alcohol on Gastric Cancer Cell Based on Network Pharmacology Frontiers in Oncology gastric cancer network pharmacology proliferation apoptosis Patchouli alcohol |
| title | Regulatory Mechanism and Experimental Verification of Patchouli Alcohol on Gastric Cancer Cell Based on Network Pharmacology |
| title_full | Regulatory Mechanism and Experimental Verification of Patchouli Alcohol on Gastric Cancer Cell Based on Network Pharmacology |
| title_fullStr | Regulatory Mechanism and Experimental Verification of Patchouli Alcohol on Gastric Cancer Cell Based on Network Pharmacology |
| title_full_unstemmed | Regulatory Mechanism and Experimental Verification of Patchouli Alcohol on Gastric Cancer Cell Based on Network Pharmacology |
| title_short | Regulatory Mechanism and Experimental Verification of Patchouli Alcohol on Gastric Cancer Cell Based on Network Pharmacology |
| title_sort | regulatory mechanism and experimental verification of patchouli alcohol on gastric cancer cell based on network pharmacology |
| topic | gastric cancer network pharmacology proliferation apoptosis Patchouli alcohol |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2021.711984/full |
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