Tick Populations and Molecular Analysis of <i>Anaplasma</i> Species in Ticks from the Republic of Korea
The present study was performed to survey the dominant tick populations and molecularly determine the pathogenic agents of anaplasmosis in ticks from Gyeongsang, Republic of Korea. A total of 3825 questing ticks were collected by the flagging method from 12 sites near animal farms in Gyeongsang from...
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MDPI AG
2023-03-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/820 |
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| author | Min-Goo Seo Haeseung Lee Badriah Alkathiri KyuSung Ahn Seung-Hun Lee SungShik Shin Seulgi Bae Kyoo-Tae Kim Min Jang Sang-Kwon Lee Yun Sang Cho Kyung-Yeon Eo Oh-Deog Kwon Dongmi Kwak |
| author_facet | Min-Goo Seo Haeseung Lee Badriah Alkathiri KyuSung Ahn Seung-Hun Lee SungShik Shin Seulgi Bae Kyoo-Tae Kim Min Jang Sang-Kwon Lee Yun Sang Cho Kyung-Yeon Eo Oh-Deog Kwon Dongmi Kwak |
| author_sort | Min-Goo Seo |
| collection | DOAJ |
| description | The present study was performed to survey the dominant tick populations and molecularly determine the pathogenic agents of anaplasmosis in ticks from Gyeongsang, Republic of Korea. A total of 3825 questing ticks were collected by the flagging method from 12 sites near animal farms in Gyeongsang from March to October 2021. A molecular genomic study was performed with ticks stored in 70% ethanol to detect <i>Anaplasma</i> genes by the previously described method. The monthly incidence of ticks varied by developmental stages, i.e., nymphs, adults, and larvae, and each of their populations peaked in May, March, and October, respectively. The predominant tick species were <i>Haemaphysalis longicornis, Haemaphysalis</i> sp., <i>Haemaphysalis flava, Ixodes nipponensis</i>, and <i>Amblyomma testudinarium</i> in order. To determine the <i>Anaplasma</i> infection rate, collected ticks were pooled into 395 groups. The minimum infection rate (MIR) of <i>Anaplasma</i> was 0.7% (27 pools). That of <i>A. phagocytophilum</i> was highest (23 pools, MIR 0.6%), followed by <i>A. phagocytophilum</i>-like <i>Anaplasma</i> spp. clade B (2 pools, MIR 0.1%), <i>A. bovis</i> (1 pool, MIR 0.1%), and <i>A. capra</i> (1 pool, MIR 0.1%), respectively. In this study, five species of ticks, including unidentified <i>Haemaphysalis</i> species, were collected in 12 survey sites in Gyeongsang, but their prevalence was somewhat different according to the tick species and survey sites. Further, the incidence rate (6.8%) of 4 <i>Anaplasma</i> spp. was not as high in tick pools. However, the results of this study may offer a basis for future epidemiological research and risk assessment of tick-borne diseases. |
| first_indexed | 2024-03-11T04:43:16Z |
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| id | doaj.art-1ea6b294298f4874b5eb0a36786d6df9 |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T04:43:16Z |
| publishDate | 2023-03-01 |
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| series | Microorganisms |
| spelling | doaj.art-1ea6b294298f4874b5eb0a36786d6df92023-11-17T20:31:00ZengMDPI AGMicroorganisms2076-26072023-03-0111482010.3390/microorganisms11040820Tick Populations and Molecular Analysis of <i>Anaplasma</i> Species in Ticks from the Republic of KoreaMin-Goo Seo0Haeseung Lee1Badriah Alkathiri2KyuSung Ahn3Seung-Hun Lee4SungShik Shin5Seulgi Bae6Kyoo-Tae Kim7Min Jang8Sang-Kwon Lee9Yun Sang Cho10Kyung-Yeon Eo11Oh-Deog Kwon12Dongmi Kwak13College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Parasitology, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of KoreaCollege of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Parasitology, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaAnimal and Plant Quarantine Agency, Gimcheon 39660, Republic of KoreaDepartment of Animal Health and Welfare, College of Healthcare and Biotechnology, Semyung University, Jecheon 27136, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaCollege of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaThe present study was performed to survey the dominant tick populations and molecularly determine the pathogenic agents of anaplasmosis in ticks from Gyeongsang, Republic of Korea. A total of 3825 questing ticks were collected by the flagging method from 12 sites near animal farms in Gyeongsang from March to October 2021. A molecular genomic study was performed with ticks stored in 70% ethanol to detect <i>Anaplasma</i> genes by the previously described method. The monthly incidence of ticks varied by developmental stages, i.e., nymphs, adults, and larvae, and each of their populations peaked in May, March, and October, respectively. The predominant tick species were <i>Haemaphysalis longicornis, Haemaphysalis</i> sp., <i>Haemaphysalis flava, Ixodes nipponensis</i>, and <i>Amblyomma testudinarium</i> in order. To determine the <i>Anaplasma</i> infection rate, collected ticks were pooled into 395 groups. The minimum infection rate (MIR) of <i>Anaplasma</i> was 0.7% (27 pools). That of <i>A. phagocytophilum</i> was highest (23 pools, MIR 0.6%), followed by <i>A. phagocytophilum</i>-like <i>Anaplasma</i> spp. clade B (2 pools, MIR 0.1%), <i>A. bovis</i> (1 pool, MIR 0.1%), and <i>A. capra</i> (1 pool, MIR 0.1%), respectively. In this study, five species of ticks, including unidentified <i>Haemaphysalis</i> species, were collected in 12 survey sites in Gyeongsang, but their prevalence was somewhat different according to the tick species and survey sites. Further, the incidence rate (6.8%) of 4 <i>Anaplasma</i> spp. was not as high in tick pools. However, the results of this study may offer a basis for future epidemiological research and risk assessment of tick-borne diseases.https://www.mdpi.com/2076-2607/11/4/820<i>Anaplasma phagocytophilum</i><i>Anaplasma phagocytophilum</i>-like <i>Anaplasma</i> spp.<i>Anaplasma bovis</i><i>Anaplasma capra</i>questing tick |
| spellingShingle | Min-Goo Seo Haeseung Lee Badriah Alkathiri KyuSung Ahn Seung-Hun Lee SungShik Shin Seulgi Bae Kyoo-Tae Kim Min Jang Sang-Kwon Lee Yun Sang Cho Kyung-Yeon Eo Oh-Deog Kwon Dongmi Kwak Tick Populations and Molecular Analysis of <i>Anaplasma</i> Species in Ticks from the Republic of Korea Microorganisms <i>Anaplasma phagocytophilum</i> <i>Anaplasma phagocytophilum</i>-like <i>Anaplasma</i> spp. <i>Anaplasma bovis</i> <i>Anaplasma capra</i> questing tick |
| title | Tick Populations and Molecular Analysis of <i>Anaplasma</i> Species in Ticks from the Republic of Korea |
| title_full | Tick Populations and Molecular Analysis of <i>Anaplasma</i> Species in Ticks from the Republic of Korea |
| title_fullStr | Tick Populations and Molecular Analysis of <i>Anaplasma</i> Species in Ticks from the Republic of Korea |
| title_full_unstemmed | Tick Populations and Molecular Analysis of <i>Anaplasma</i> Species in Ticks from the Republic of Korea |
| title_short | Tick Populations and Molecular Analysis of <i>Anaplasma</i> Species in Ticks from the Republic of Korea |
| title_sort | tick populations and molecular analysis of i anaplasma i species in ticks from the republic of korea |
| topic | <i>Anaplasma phagocytophilum</i> <i>Anaplasma phagocytophilum</i>-like <i>Anaplasma</i> spp. <i>Anaplasma bovis</i> <i>Anaplasma capra</i> questing tick |
| url | https://www.mdpi.com/2076-2607/11/4/820 |
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