Reduced expression in preterm birth of sFLT-1 and PlGF with a high sFLT-1/PlGF ratio in extracellular vesicles suggests a potential biomarker
Preterm birth may have a pathological impact on intrauterine development of the fetal brain, resulting in developmental disabilities. In this study, we examine the expression of soluble Fms-like tyrosine kinase 1 (sFLT-1) and placental growth factor (PlGF), which is one of the vascular endothelial g...
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| Format: | Article |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.1024587/full |
| _version_ | 1797979289226838016 |
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| author | Sama Hussein Weina Ju Stephanie Pizzella Michael Flory Chu Chu Yong Wang Nanbert Zhong |
| author_facet | Sama Hussein Weina Ju Stephanie Pizzella Michael Flory Chu Chu Yong Wang Nanbert Zhong |
| author_sort | Sama Hussein |
| collection | DOAJ |
| description | Preterm birth may have a pathological impact on intrauterine development of the fetal brain, resulting in developmental disabilities. In this study, we examine the expression of soluble Fms-like tyrosine kinase 1 (sFLT-1) and placental growth factor (PlGF), which is one of the vascular endothelial growth factors (VEGFs), as these play a key role in angiogenesis; in particular, we examine their effect on the sFLT-1/PlGF ratio in cases of preterm birth as compared to typical pregnancies. Enzyme-linked immunosorbent assay was performed on samples of maternal-derived plasma and extracellular vesicles-exosomes (EVs-EXs) isolated at the third trimester, consisting of 17 samples from cases of preterm birth and 38 control cases. Our results showed that both sFLT-1 (P=0.0014) and PlGF (P=0.0032) were significantly downregulated in cases of preterm birth compared to controls, while the sFLT-1/PIGF ratio was significantly (P=0.0008) increased in EVs-EXs, but not in maternal plasma. Our results suggest that this reduced expression of sFLT-1 and PlGF with an elevated sFLT-1/PlGF ratio in EVs-EXs may represent a potential biomarker for prediction of PTB. |
| first_indexed | 2024-04-11T05:36:38Z |
| format | Article |
| id | doaj.art-1ea8fc20a7a0405397a8382f73e2e855 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-11T05:36:38Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-1ea8fc20a7a0405397a8382f73e2e8552022-12-22T11:24:43ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-12-011310.3389/fendo.2022.10245871024587Reduced expression in preterm birth of sFLT-1 and PlGF with a high sFLT-1/PlGF ratio in extracellular vesicles suggests a potential biomarkerSama Hussein0Weina Ju1Stephanie Pizzella2Michael Flory3Chu Chu4Yong Wang5Nanbert Zhong6New York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, United StatesNew York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, United StatesDepartment of Obstetrics and Gynecology, School of Medicine, Washington University, St. Louis, MO, United StatesNew York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, United StatesNew York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, United StatesDepartment of Obstetrics and Gynecology, School of Medicine, Washington University, St. Louis, MO, United StatesNew York State Institute for Basic Research in Developmental Disabilities, Staten Island, NY, United StatesPreterm birth may have a pathological impact on intrauterine development of the fetal brain, resulting in developmental disabilities. In this study, we examine the expression of soluble Fms-like tyrosine kinase 1 (sFLT-1) and placental growth factor (PlGF), which is one of the vascular endothelial growth factors (VEGFs), as these play a key role in angiogenesis; in particular, we examine their effect on the sFLT-1/PlGF ratio in cases of preterm birth as compared to typical pregnancies. Enzyme-linked immunosorbent assay was performed on samples of maternal-derived plasma and extracellular vesicles-exosomes (EVs-EXs) isolated at the third trimester, consisting of 17 samples from cases of preterm birth and 38 control cases. Our results showed that both sFLT-1 (P=0.0014) and PlGF (P=0.0032) were significantly downregulated in cases of preterm birth compared to controls, while the sFLT-1/PIGF ratio was significantly (P=0.0008) increased in EVs-EXs, but not in maternal plasma. Our results suggest that this reduced expression of sFLT-1 and PlGF with an elevated sFLT-1/PlGF ratio in EVs-EXs may represent a potential biomarker for prediction of PTB.https://www.frontiersin.org/articles/10.3389/fendo.2022.1024587/fullsFlt-1placental growth factorbiomarkerpreterm (birth)PTBextracellular vehicles (EVs) |
| spellingShingle | Sama Hussein Weina Ju Stephanie Pizzella Michael Flory Chu Chu Yong Wang Nanbert Zhong Reduced expression in preterm birth of sFLT-1 and PlGF with a high sFLT-1/PlGF ratio in extracellular vesicles suggests a potential biomarker Frontiers in Endocrinology sFlt-1 placental growth factor biomarker preterm (birth) PTB extracellular vehicles (EVs) |
| title | Reduced expression in preterm birth of sFLT-1 and PlGF with a high sFLT-1/PlGF ratio in extracellular vesicles suggests a potential biomarker |
| title_full | Reduced expression in preterm birth of sFLT-1 and PlGF with a high sFLT-1/PlGF ratio in extracellular vesicles suggests a potential biomarker |
| title_fullStr | Reduced expression in preterm birth of sFLT-1 and PlGF with a high sFLT-1/PlGF ratio in extracellular vesicles suggests a potential biomarker |
| title_full_unstemmed | Reduced expression in preterm birth of sFLT-1 and PlGF with a high sFLT-1/PlGF ratio in extracellular vesicles suggests a potential biomarker |
| title_short | Reduced expression in preterm birth of sFLT-1 and PlGF with a high sFLT-1/PlGF ratio in extracellular vesicles suggests a potential biomarker |
| title_sort | reduced expression in preterm birth of sflt 1 and plgf with a high sflt 1 plgf ratio in extracellular vesicles suggests a potential biomarker |
| topic | sFlt-1 placental growth factor biomarker preterm (birth) PTB extracellular vehicles (EVs) |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.1024587/full |
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