An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model
Sargassum muticum is a brown seaweed with strong potential to be used as a functional food ingredient, mainly due to its antioxidant properties. It is widely used in traditional oriental medicine for the treatment of numerous diseases. Nevertheless, few studies have been conducted to add scientific...
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| Format: | Article |
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MDPI AG
2017-11-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/15/11/353 |
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| author | Susete Pinteus Marco F. L. Lemos Joana Silva Celso Alves Agnieszka Neugebauer Rafaela Freitas Adriana Duarte Rui Pedrosa |
| author_facet | Susete Pinteus Marco F. L. Lemos Joana Silva Celso Alves Agnieszka Neugebauer Rafaela Freitas Adriana Duarte Rui Pedrosa |
| author_sort | Susete Pinteus |
| collection | DOAJ |
| description | Sargassum muticum is a brown seaweed with strong potential to be used as a functional food ingredient, mainly due to its antioxidant properties. It is widely used in traditional oriental medicine for the treatment of numerous diseases. Nevertheless, few studies have been conducted to add scientific evidence on its effects as well as on the mechanisms of action involved. In this work, the human cell line MCF-7 was used as an in vitro cellular model to evaluate the capability of Sargassum muticum enriched fractions to protect cells on an oxidative stress condition. The concentration of the bioactive compounds was obtained by vacuum liquid chromatography applied on methanol (M) and 1:1 methanol:dichloromethane (MD) crude extracts, resulting in seven enriched fractions from the M extraction (MF2–MF8), and eight fractions from the MD extraction (MDF1–MDF8). All fractions were tested for cytotoxic properties on MCF-7 cells and the nontoxic ones were tested for their capacity to blunt the damaging effects of hydrogen peroxide-induced oxidative stress. The nontoxic effects were also confirmed in 3T3 fibroblast cells as a nontumor cell line. The antioxidant potential of each fraction, as well as changes in the cell’s real-time hydrogen peroxide production, in the mitochondrial membrane potential, and in Caspase-9 activity were evaluated. The results suggest that the protective effects evidenced by S. muticum can be related with the inhibition of hydrogen peroxide production and the inhibition of Caspase-9 activity. |
| first_indexed | 2024-12-10T07:21:12Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-12-10T07:21:12Z |
| publishDate | 2017-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-1eab14e649cd45f4a7ff12c22a2118f02022-12-22T01:57:49ZengMDPI AGMarine Drugs1660-33972017-11-01151135310.3390/md15110353md15110353An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro ModelSusete Pinteus0Marco F. L. Lemos1Joana Silva2Celso Alves3Agnieszka Neugebauer4Rafaela Freitas5Adriana Duarte6Rui Pedrosa7MARE—Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, PortugalMARE—Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, PortugalMARE—Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, PortugalMARE—Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, PortugalMARE—Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, PortugalMARE—Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, PortugalMARE—Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, PortugalMARE—Marine and Environmental Sciences Centre, School of Tourism and Maritime Technology, Polytechnic Institute of Leiria, 2520-641 Peniche, PortugalSargassum muticum is a brown seaweed with strong potential to be used as a functional food ingredient, mainly due to its antioxidant properties. It is widely used in traditional oriental medicine for the treatment of numerous diseases. Nevertheless, few studies have been conducted to add scientific evidence on its effects as well as on the mechanisms of action involved. In this work, the human cell line MCF-7 was used as an in vitro cellular model to evaluate the capability of Sargassum muticum enriched fractions to protect cells on an oxidative stress condition. The concentration of the bioactive compounds was obtained by vacuum liquid chromatography applied on methanol (M) and 1:1 methanol:dichloromethane (MD) crude extracts, resulting in seven enriched fractions from the M extraction (MF2–MF8), and eight fractions from the MD extraction (MDF1–MDF8). All fractions were tested for cytotoxic properties on MCF-7 cells and the nontoxic ones were tested for their capacity to blunt the damaging effects of hydrogen peroxide-induced oxidative stress. The nontoxic effects were also confirmed in 3T3 fibroblast cells as a nontumor cell line. The antioxidant potential of each fraction, as well as changes in the cell’s real-time hydrogen peroxide production, in the mitochondrial membrane potential, and in Caspase-9 activity were evaluated. The results suggest that the protective effects evidenced by S. muticum can be related with the inhibition of hydrogen peroxide production and the inhibition of Caspase-9 activity.https://www.mdpi.com/1660-3397/15/11/353antioxidant compoundsbrown algaeCaspase-9cell death mechanismscytoprotectionmarine natural compoundsMCF-7 cellsmitochondrial membrane potentialreactive oxygen species (ROS)seaweed |
| spellingShingle | Susete Pinteus Marco F. L. Lemos Joana Silva Celso Alves Agnieszka Neugebauer Rafaela Freitas Adriana Duarte Rui Pedrosa An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model Marine Drugs antioxidant compounds brown algae Caspase-9 cell death mechanisms cytoprotection marine natural compounds MCF-7 cells mitochondrial membrane potential reactive oxygen species (ROS) seaweed |
| title | An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model |
| title_full | An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model |
| title_fullStr | An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model |
| title_full_unstemmed | An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model |
| title_short | An Insight into Sargassum muticum Cytoprotective Mechanisms against Oxidative Stress on a Human Cell In Vitro Model |
| title_sort | insight into sargassum muticum cytoprotective mechanisms against oxidative stress on a human cell in vitro model |
| topic | antioxidant compounds brown algae Caspase-9 cell death mechanisms cytoprotection marine natural compounds MCF-7 cells mitochondrial membrane potential reactive oxygen species (ROS) seaweed |
| url | https://www.mdpi.com/1660-3397/15/11/353 |
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