The ERM protein Moesin is essential for neuronal morphogenesis and long-term memory in Drosophila
Abstract Moesin is a cytoskeletal adaptor protein that plays an important role in modification of the actin cytoskeleton. Rearrangement of the actin cytoskeleton drives both neuronal morphogenesis and the structural changes in neurons that are required for long-term memory formation. Moesin has been...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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BMC
2017-08-01
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| Series: | Molecular Brain |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13041-017-0322-y |
| _version_ | 1818825925664440320 |
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| author | Patrick S. Freymuth Helen L. Fitzsimons |
| author_facet | Patrick S. Freymuth Helen L. Fitzsimons |
| author_sort | Patrick S. Freymuth |
| collection | DOAJ |
| description | Abstract Moesin is a cytoskeletal adaptor protein that plays an important role in modification of the actin cytoskeleton. Rearrangement of the actin cytoskeleton drives both neuronal morphogenesis and the structural changes in neurons that are required for long-term memory formation. Moesin has been identified as a candidate memory gene in Drosophila, however, whether it is required for memory formation has not been evaluated. Here, we investigate the role of Moesin in neuronal morphogenesis and in short- and long-term memory formation in the courtship suppression assay, a model of associative memory. We found that both knockdown and overexpression of Moesin led to defects in axon growth and guidance as well as dendritic arborization. Moreover, reduction of Moesin expression or expression of a constitutively active phosphomimetic in the adult Drosophila brain had no effect on short term memory, but prevented long-term memory formation, an effect that was independent of its role in development. These results indicate a critical role for Moesin in both neuronal morphogenesis and long-term memory formation. |
| first_indexed | 2024-12-19T00:19:31Z |
| format | Article |
| id | doaj.art-1eab883986724653b5892da84dc6729b |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-12-19T00:19:31Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-1eab883986724653b5892da84dc6729b2022-12-21T20:45:37ZengBMCMolecular Brain1756-66062017-08-0110111310.1186/s13041-017-0322-yThe ERM protein Moesin is essential for neuronal morphogenesis and long-term memory in DrosophilaPatrick S. Freymuth0Helen L. Fitzsimons1Institute of Fundamental Sciences, Massey UniversityInstitute of Fundamental Sciences, Massey UniversityAbstract Moesin is a cytoskeletal adaptor protein that plays an important role in modification of the actin cytoskeleton. Rearrangement of the actin cytoskeleton drives both neuronal morphogenesis and the structural changes in neurons that are required for long-term memory formation. Moesin has been identified as a candidate memory gene in Drosophila, however, whether it is required for memory formation has not been evaluated. Here, we investigate the role of Moesin in neuronal morphogenesis and in short- and long-term memory formation in the courtship suppression assay, a model of associative memory. We found that both knockdown and overexpression of Moesin led to defects in axon growth and guidance as well as dendritic arborization. Moreover, reduction of Moesin expression or expression of a constitutively active phosphomimetic in the adult Drosophila brain had no effect on short term memory, but prevented long-term memory formation, an effect that was independent of its role in development. These results indicate a critical role for Moesin in both neuronal morphogenesis and long-term memory formation.http://link.springer.com/article/10.1186/s13041-017-0322-yMoesinEzrinRadixinERMCytoskeletonActin |
| spellingShingle | Patrick S. Freymuth Helen L. Fitzsimons The ERM protein Moesin is essential for neuronal morphogenesis and long-term memory in Drosophila Molecular Brain Moesin Ezrin Radixin ERM Cytoskeleton Actin |
| title | The ERM protein Moesin is essential for neuronal morphogenesis and long-term memory in Drosophila |
| title_full | The ERM protein Moesin is essential for neuronal morphogenesis and long-term memory in Drosophila |
| title_fullStr | The ERM protein Moesin is essential for neuronal morphogenesis and long-term memory in Drosophila |
| title_full_unstemmed | The ERM protein Moesin is essential for neuronal morphogenesis and long-term memory in Drosophila |
| title_short | The ERM protein Moesin is essential for neuronal morphogenesis and long-term memory in Drosophila |
| title_sort | erm protein moesin is essential for neuronal morphogenesis and long term memory in drosophila |
| topic | Moesin Ezrin Radixin ERM Cytoskeleton Actin |
| url | http://link.springer.com/article/10.1186/s13041-017-0322-y |
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