Novel Antibiotic Resistance Genes Identified by Functional Gene Library Screening in <i>Stenotrophomonas maltophilia</i> and <i>Chryseobacterium</i> spp. Bacteria of Soil Origin
As one of the most diverse habitats of microorganisms, soil has been recognised as a reservoir of both antibiotics and the antibiotic resistance genes (ARGs). Bacteria naturally inhabiting soil or water often possess innate ARGs to counteract the chemical compounds produced by competitors living in...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/7/6037 |
| _version_ | 1797607863387947008 |
|---|---|
| author | Laurita Klimkaitė Ignas Ragaišis Renatas Krasauskas Modestas Ružauskas Edita Sužiedėlienė Julija Armalytė |
| author_facet | Laurita Klimkaitė Ignas Ragaišis Renatas Krasauskas Modestas Ružauskas Edita Sužiedėlienė Julija Armalytė |
| author_sort | Laurita Klimkaitė |
| collection | DOAJ |
| description | As one of the most diverse habitats of microorganisms, soil has been recognised as a reservoir of both antibiotics and the antibiotic resistance genes (ARGs). Bacteria naturally inhabiting soil or water often possess innate ARGs to counteract the chemical compounds produced by competitors living in the same environment. When such bacteria are able to cause infections in immunocompromised patients, their strong innate antibiotic resistance mechanisms make treatment difficult. We generated functional gene libraries using antibiotic-resistant <i>Stenotrophomonas maltophilia</i> and <i>Chryseobacterium</i> spp. bacteria isolated from agricultural soils in Lithuania to select for the genetic determinants responsible for their resistance. We were able to find novel variants of aminoglycoside and β-lactam resistance genes, with β-lactamases isolated from the <i>Chryseobacterium</i> spp. functional gene library, one of which is a variant of IND-like metallo-β-lactamase (MBL) IND-17 and the other of which is a previously uncharacterised MBL we named CHM (<i>Chryseobacterium</i> metallo β-lactamase). Our results indicate that soil microorganisms possess a diversity of ARG variants, which could potentially be transferred to the clinical setting. |
| first_indexed | 2024-03-11T05:36:33Z |
| format | Article |
| id | doaj.art-1eabc7d3cde54d439d13b756c389f7f2 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T05:36:33Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1eabc7d3cde54d439d13b756c389f7f22023-11-17T16:45:30ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01247603710.3390/ijms24076037Novel Antibiotic Resistance Genes Identified by Functional Gene Library Screening in <i>Stenotrophomonas maltophilia</i> and <i>Chryseobacterium</i> spp. Bacteria of Soil OriginLaurita Klimkaitė0Ignas Ragaišis1Renatas Krasauskas2Modestas Ružauskas3Edita Sužiedėlienė4Julija Armalytė5Institute of Biosciences, Life Sciences Center, Vilnius University, 10257 Vilnius, LithuaniaInstitute of Biosciences, Life Sciences Center, Vilnius University, 10257 Vilnius, LithuaniaInstitute of Biosciences, Life Sciences Center, Vilnius University, 10257 Vilnius, LithuaniaMicrobiology and Virology Institute, Lithuanian University of Health Sciences, 44307 Kaunas, LithuaniaInstitute of Biosciences, Life Sciences Center, Vilnius University, 10257 Vilnius, LithuaniaInstitute of Biosciences, Life Sciences Center, Vilnius University, 10257 Vilnius, LithuaniaAs one of the most diverse habitats of microorganisms, soil has been recognised as a reservoir of both antibiotics and the antibiotic resistance genes (ARGs). Bacteria naturally inhabiting soil or water often possess innate ARGs to counteract the chemical compounds produced by competitors living in the same environment. When such bacteria are able to cause infections in immunocompromised patients, their strong innate antibiotic resistance mechanisms make treatment difficult. We generated functional gene libraries using antibiotic-resistant <i>Stenotrophomonas maltophilia</i> and <i>Chryseobacterium</i> spp. bacteria isolated from agricultural soils in Lithuania to select for the genetic determinants responsible for their resistance. We were able to find novel variants of aminoglycoside and β-lactam resistance genes, with β-lactamases isolated from the <i>Chryseobacterium</i> spp. functional gene library, one of which is a variant of IND-like metallo-β-lactamase (MBL) IND-17 and the other of which is a previously uncharacterised MBL we named CHM (<i>Chryseobacterium</i> metallo β-lactamase). Our results indicate that soil microorganisms possess a diversity of ARG variants, which could potentially be transferred to the clinical setting.https://www.mdpi.com/1422-0067/24/7/6037<i>Stenotrophomonas maltophilia</i><i>Chryseobacterium</i> spp.functional gene libraryantibiotic resistance genesantibiotic resistance in soilmetallo-β-lactamase |
| spellingShingle | Laurita Klimkaitė Ignas Ragaišis Renatas Krasauskas Modestas Ružauskas Edita Sužiedėlienė Julija Armalytė Novel Antibiotic Resistance Genes Identified by Functional Gene Library Screening in <i>Stenotrophomonas maltophilia</i> and <i>Chryseobacterium</i> spp. Bacteria of Soil Origin International Journal of Molecular Sciences <i>Stenotrophomonas maltophilia</i> <i>Chryseobacterium</i> spp. functional gene library antibiotic resistance genes antibiotic resistance in soil metallo-β-lactamase |
| title | Novel Antibiotic Resistance Genes Identified by Functional Gene Library Screening in <i>Stenotrophomonas maltophilia</i> and <i>Chryseobacterium</i> spp. Bacteria of Soil Origin |
| title_full | Novel Antibiotic Resistance Genes Identified by Functional Gene Library Screening in <i>Stenotrophomonas maltophilia</i> and <i>Chryseobacterium</i> spp. Bacteria of Soil Origin |
| title_fullStr | Novel Antibiotic Resistance Genes Identified by Functional Gene Library Screening in <i>Stenotrophomonas maltophilia</i> and <i>Chryseobacterium</i> spp. Bacteria of Soil Origin |
| title_full_unstemmed | Novel Antibiotic Resistance Genes Identified by Functional Gene Library Screening in <i>Stenotrophomonas maltophilia</i> and <i>Chryseobacterium</i> spp. Bacteria of Soil Origin |
| title_short | Novel Antibiotic Resistance Genes Identified by Functional Gene Library Screening in <i>Stenotrophomonas maltophilia</i> and <i>Chryseobacterium</i> spp. Bacteria of Soil Origin |
| title_sort | novel antibiotic resistance genes identified by functional gene library screening in i stenotrophomonas maltophilia i and i chryseobacterium i spp bacteria of soil origin |
| topic | <i>Stenotrophomonas maltophilia</i> <i>Chryseobacterium</i> spp. functional gene library antibiotic resistance genes antibiotic resistance in soil metallo-β-lactamase |
| url | https://www.mdpi.com/1422-0067/24/7/6037 |
| work_keys_str_mv | AT lauritaklimkaite novelantibioticresistancegenesidentifiedbyfunctionalgenelibraryscreeninginistenotrophomonasmaltophiliaiandichryseobacteriumisppbacteriaofsoilorigin AT ignasragaisis novelantibioticresistancegenesidentifiedbyfunctionalgenelibraryscreeninginistenotrophomonasmaltophiliaiandichryseobacteriumisppbacteriaofsoilorigin AT renataskrasauskas novelantibioticresistancegenesidentifiedbyfunctionalgenelibraryscreeninginistenotrophomonasmaltophiliaiandichryseobacteriumisppbacteriaofsoilorigin AT modestasruzauskas novelantibioticresistancegenesidentifiedbyfunctionalgenelibraryscreeninginistenotrophomonasmaltophiliaiandichryseobacteriumisppbacteriaofsoilorigin AT editasuziedeliene novelantibioticresistancegenesidentifiedbyfunctionalgenelibraryscreeninginistenotrophomonasmaltophiliaiandichryseobacteriumisppbacteriaofsoilorigin AT julijaarmalyte novelantibioticresistancegenesidentifiedbyfunctionalgenelibraryscreeninginistenotrophomonasmaltophiliaiandichryseobacteriumisppbacteriaofsoilorigin |