Arsenite Oxidation and Arsenite Resistance by Bacillus sp. PNKP-S2
Arsenic causes human health problems after accumulate in the body for 10-15 years and arsenite [As(III)] is generally regarded as being more mobile and toxic than other oxidation states. In this study, two-hundred and three bacterial strains were isolated from groundwater and soil samples collecting...
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| Format: | Article |
| Language: | English |
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Thai Society of Higher Eduction Institutes on Environment
2015-01-01
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| Series: | EnvironmentAsia |
| Subjects: | |
| Online Access: | http://www.tshe.org/ea/pdf/vol8no1-02.pdf |
| _version_ | 1811262706206900224 |
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| author | Pranee Pattanapipitpaisal Natanong Yodsing Rungpha Santhaweesuk Phitthaya Wamakhan |
| author_facet | Pranee Pattanapipitpaisal Natanong Yodsing Rungpha Santhaweesuk Phitthaya Wamakhan |
| author_sort | Pranee Pattanapipitpaisal |
| collection | DOAJ |
| description | Arsenic causes human health problems after accumulate in the body for 10-15 years and arsenite [As(III)] is generally regarded as being more mobile and toxic than other oxidation states. In this study, two-hundred and three bacterial strains were isolated from groundwater and soil samples collecting in Ubon Ratchathani Province, Thailand. All strains were screened for arsenic tolerant efficiency at 1-10 mM of sodium arsenite. Eighteen selected strains which had the highest resistance to 10 mM of As(III) were further studied for their As(III)-oxidizing activity and growth in enrichment and growth medium (EG medium) supplemented with 0.58 mM of As(III). It was found that strain PNKP-S2 was able to grow in the medium with As(III) as a sole energy source and had 89.11% As(III) removal within 48 h. The PCR-based 16S rDNA sequencing analysis revealed that the strain PNKP-S2 was closed relative to Bacillus sp. This is the first report on Bacillus sp. chemolithoautotrophic As(III)-oxidizer and this strain could be a potential candidate for application in arsenic remediation of contaminated water. |
| first_indexed | 2024-04-12T19:31:37Z |
| format | Article |
| id | doaj.art-1ead7607869f4bb4b79faa3e207f6474 |
| institution | Directory Open Access Journal |
| issn | 1906-1714 |
| language | English |
| last_indexed | 2024-04-12T19:31:37Z |
| publishDate | 2015-01-01 |
| publisher | Thai Society of Higher Eduction Institutes on Environment |
| record_format | Article |
| series | EnvironmentAsia |
| spelling | doaj.art-1ead7607869f4bb4b79faa3e207f64742022-12-22T03:19:20ZengThai Society of Higher Eduction Institutes on EnvironmentEnvironmentAsia1906-17142015-01-0181915Arsenite Oxidation and Arsenite Resistance by Bacillus sp. PNKP-S2Pranee Pattanapipitpaisal0Natanong Yodsing1Rungpha Santhaweesuk2Phitthaya Wamakhan3Bioremediation Laboratory Unit, Faculty of Science, Ubon Ratchathani University,Ubon Ratchathani 34190, ThailandBioremediation Laboratory Unit, Faculty of Science, Ubon Ratchathani University,Ubon Ratchathani 34190, ThailandBioremediation Laboratory Unit, Faculty of Science, Ubon Ratchathani University,Ubon Ratchathani 34190, ThailandBioremediation Laboratory Unit, Faculty of Science, Ubon Ratchathani University,Ubon Ratchathani 34190, ThailandArsenic causes human health problems after accumulate in the body for 10-15 years and arsenite [As(III)] is generally regarded as being more mobile and toxic than other oxidation states. In this study, two-hundred and three bacterial strains were isolated from groundwater and soil samples collecting in Ubon Ratchathani Province, Thailand. All strains were screened for arsenic tolerant efficiency at 1-10 mM of sodium arsenite. Eighteen selected strains which had the highest resistance to 10 mM of As(III) were further studied for their As(III)-oxidizing activity and growth in enrichment and growth medium (EG medium) supplemented with 0.58 mM of As(III). It was found that strain PNKP-S2 was able to grow in the medium with As(III) as a sole energy source and had 89.11% As(III) removal within 48 h. The PCR-based 16S rDNA sequencing analysis revealed that the strain PNKP-S2 was closed relative to Bacillus sp. This is the first report on Bacillus sp. chemolithoautotrophic As(III)-oxidizer and this strain could be a potential candidate for application in arsenic remediation of contaminated water.http://www.tshe.org/ea/pdf/vol8no1-02.pdfarsenic; arsenitearsenite-oxidizing bacteriumarsenic-tolerant bacteriaBacillus sp. |
| spellingShingle | Pranee Pattanapipitpaisal Natanong Yodsing Rungpha Santhaweesuk Phitthaya Wamakhan Arsenite Oxidation and Arsenite Resistance by Bacillus sp. PNKP-S2 EnvironmentAsia arsenic; arsenite arsenite-oxidizing bacterium arsenic-tolerant bacteria Bacillus sp. |
| title | Arsenite Oxidation and Arsenite Resistance by Bacillus sp. PNKP-S2 |
| title_full | Arsenite Oxidation and Arsenite Resistance by Bacillus sp. PNKP-S2 |
| title_fullStr | Arsenite Oxidation and Arsenite Resistance by Bacillus sp. PNKP-S2 |
| title_full_unstemmed | Arsenite Oxidation and Arsenite Resistance by Bacillus sp. PNKP-S2 |
| title_short | Arsenite Oxidation and Arsenite Resistance by Bacillus sp. PNKP-S2 |
| title_sort | arsenite oxidation and arsenite resistance by bacillus sp pnkp s2 |
| topic | arsenic; arsenite arsenite-oxidizing bacterium arsenic-tolerant bacteria Bacillus sp. |
| url | http://www.tshe.org/ea/pdf/vol8no1-02.pdf |
| work_keys_str_mv | AT praneepattanapipitpaisal arseniteoxidationandarseniteresistancebybacillussppnkps2 AT natanongyodsing arseniteoxidationandarseniteresistancebybacillussppnkps2 AT rungphasanthaweesuk arseniteoxidationandarseniteresistancebybacillussppnkps2 AT phitthayawamakhan arseniteoxidationandarseniteresistancebybacillussppnkps2 |