Environmental concentrations of surfactants as a trigger for climax of horizonal gene transfer of antibiotic resistance
Ubiquitous antibiotic resistance genes (ARGs) is a significant global human health concern. Surfactants have been extensively used worldwide, and the consumption of surfactants containing hygiene, cleaning agents and disinfectants was multiplied during COVID-19 pandemic, which have caused significan...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023042421 |
| _version_ | 1797807028181139456 |
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| author | Xiaonan Wang Gaoquan Du Zhuang Qiao Yixuan Yang Huimin Shi Daoyong Zhang Xiangliang Pan |
| author_facet | Xiaonan Wang Gaoquan Du Zhuang Qiao Yixuan Yang Huimin Shi Daoyong Zhang Xiangliang Pan |
| author_sort | Xiaonan Wang |
| collection | DOAJ |
| description | Ubiquitous antibiotic resistance genes (ARGs) is a significant global human health concern. Surfactants have been extensively used worldwide, and the consumption of surfactants containing hygiene, cleaning agents and disinfectants was multiplied during COVID-19 pandemic, which have caused significantly increased pollution of surfactants in aquatic environment. Whether such ever-increasing surfactant concentration boost dissemination risk of ARGs still remains unknown. Here the effects of three typical surfactants such as sodium dodecyl sulfate, cetyltrimethylammonium bromide and benzalkonium chloride on the transformation of pUC19 plasmid (2686 bp)-borne ARGs to recipient bacteria E. coli DH5ɑ were investigated. It was found that these surfactants at environmental concentrations facilitated horizonal gene transfer (HGT) via transformation. The transformation triggering concentrations for the three surfactants were 0.25–0.34 mg/L with a maximum increased transformation frequency of 13.51–22.93-fold. The mechanisms involved in activated HGT of ARGs via transformation triggered by surfactants could be mainly attributed to the increased production of reactive oxygen species, which further enhanced cell membrane permeability. These findings provide new sights for understanding of ARG propagation and also imply that the drastic rise of surfactant concentration in aquatic environment may significantly increase the dissemination risk of antibiotic resistance. |
| first_indexed | 2024-03-13T06:16:21Z |
| format | Article |
| id | doaj.art-1eb0072f347647d8930854aaaa8e4c52 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-13T06:16:21Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-1eb0072f347647d8930854aaaa8e4c522023-06-11T04:14:46ZengElsevierHeliyon2405-84402023-06-0196e17034Environmental concentrations of surfactants as a trigger for climax of horizonal gene transfer of antibiotic resistanceXiaonan Wang0Gaoquan Du1Zhuang Qiao2Yixuan Yang3Huimin Shi4Daoyong Zhang5Xiangliang Pan6Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, 310014, China; School of Environment Science and Spatial Information, China University of Mining and Technology, Xuzhou, 221116, China; Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Hangzhou, 310015, China; Shaoxing Research Institute of Zhejiang University of Technology, Shaoxing, 312000, ChinaKey Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, 310014, ChinaKey Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, 310014, ChinaKey Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, 310014, ChinaKey Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, 310014, ChinaKey Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, 310014, ChinaKey Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, 310014, China; Corresponding author.Ubiquitous antibiotic resistance genes (ARGs) is a significant global human health concern. Surfactants have been extensively used worldwide, and the consumption of surfactants containing hygiene, cleaning agents and disinfectants was multiplied during COVID-19 pandemic, which have caused significantly increased pollution of surfactants in aquatic environment. Whether such ever-increasing surfactant concentration boost dissemination risk of ARGs still remains unknown. Here the effects of three typical surfactants such as sodium dodecyl sulfate, cetyltrimethylammonium bromide and benzalkonium chloride on the transformation of pUC19 plasmid (2686 bp)-borne ARGs to recipient bacteria E. coli DH5ɑ were investigated. It was found that these surfactants at environmental concentrations facilitated horizonal gene transfer (HGT) via transformation. The transformation triggering concentrations for the three surfactants were 0.25–0.34 mg/L with a maximum increased transformation frequency of 13.51–22.93-fold. The mechanisms involved in activated HGT of ARGs via transformation triggered by surfactants could be mainly attributed to the increased production of reactive oxygen species, which further enhanced cell membrane permeability. These findings provide new sights for understanding of ARG propagation and also imply that the drastic rise of surfactant concentration in aquatic environment may significantly increase the dissemination risk of antibiotic resistance.http://www.sciencedirect.com/science/article/pii/S2405844023042421Antibiotic resistance genesSurfactantsEnvironmental concentrationTransformationDissemination risk |
| spellingShingle | Xiaonan Wang Gaoquan Du Zhuang Qiao Yixuan Yang Huimin Shi Daoyong Zhang Xiangliang Pan Environmental concentrations of surfactants as a trigger for climax of horizonal gene transfer of antibiotic resistance Heliyon Antibiotic resistance genes Surfactants Environmental concentration Transformation Dissemination risk |
| title | Environmental concentrations of surfactants as a trigger for climax of horizonal gene transfer of antibiotic resistance |
| title_full | Environmental concentrations of surfactants as a trigger for climax of horizonal gene transfer of antibiotic resistance |
| title_fullStr | Environmental concentrations of surfactants as a trigger for climax of horizonal gene transfer of antibiotic resistance |
| title_full_unstemmed | Environmental concentrations of surfactants as a trigger for climax of horizonal gene transfer of antibiotic resistance |
| title_short | Environmental concentrations of surfactants as a trigger for climax of horizonal gene transfer of antibiotic resistance |
| title_sort | environmental concentrations of surfactants as a trigger for climax of horizonal gene transfer of antibiotic resistance |
| topic | Antibiotic resistance genes Surfactants Environmental concentration Transformation Dissemination risk |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023042421 |
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