Effects of Oral Commensal Streptococci on <i>Porphyromonas gingivalis</i> Invasion into Oral Epithelial Cells
The objective of this study was to determine if the interaction between common oral commensal bacteria and oral epithelial cells would provide protective effects against the invasion of periodontopathogen <i>Porphyromonas gingivalis</i>. Oral epithelial OKF6/Tert cells were used in co-cu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Dentistry Journal |
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| Online Access: | https://www.mdpi.com/2304-6767/8/2/39 |
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| author | Alyssa N. Hanel Hannah M. Herzog Michelle G. James Giancarlo A. Cuadra |
| author_facet | Alyssa N. Hanel Hannah M. Herzog Michelle G. James Giancarlo A. Cuadra |
| author_sort | Alyssa N. Hanel |
| collection | DOAJ |
| description | The objective of this study was to determine if the interaction between common oral commensal bacteria and oral epithelial cells would provide protective effects against the invasion of periodontopathogen <i>Porphyromonas gingivalis</i>. Oral epithelial OKF6/Tert cells were used in co-cultures with <i>Streptococcus gordonii</i>, <i>Streptococcus oralis</i>, <i>Streptococcus mitis</i>, and <i>Streptococcus intermedius</i>. The viability of OKF6/Tert cells following a bacterial challenge was evaluated by trypan blue exclusion. The adherence of commensal species was determined by CFU counts. <i>P. gingivalis</i> invasion in OKF6/Tert cells was assessed before and after exposure to commensal species according to CFU counts. Viability assays show that only <i>S. gordonii</i> and <i>S. intermedius</i> display low toxicity toward OKF6/Tert cells. Both commensals adhere to OKF6/Tert cells at an average ratio of 1 CFU to 10 cells. <i>P. gingivalis</i> invasion into host cells is significantly reduced by 25% or 60% after exposure to <i>S. gordonii</i> or <i>S. intermedius</i>, respectively. The results suggest that these commensal species bind to host cells and diminish <i>P. gingivalis</i> invasion. This is important in the context of periodontal disease since <i>P. gingivalis</i> primarily acts on the host by invading it. Therefore, efforts to decrease invasion will eventually lead to future therapies harnessing the mechanisms employed by oral commensal bacteria. |
| first_indexed | 2024-03-10T20:03:10Z |
| format | Article |
| id | doaj.art-1eb331597cee406788010bfbaffdb879 |
| institution | Directory Open Access Journal |
| issn | 2304-6767 |
| language | English |
| last_indexed | 2024-03-10T20:03:10Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Dentistry Journal |
| spelling | doaj.art-1eb331597cee406788010bfbaffdb8792023-11-19T23:23:01ZengMDPI AGDentistry Journal2304-67672020-05-01823910.3390/dj8020039Effects of Oral Commensal Streptococci on <i>Porphyromonas gingivalis</i> Invasion into Oral Epithelial CellsAlyssa N. Hanel0Hannah M. Herzog1Michelle G. James2Giancarlo A. Cuadra3Department of Biology, Muhlenberg College, 2400 W. Chew Street, Allentown, PA 18104, USADepartment of Biology, Muhlenberg College, 2400 W. Chew Street, Allentown, PA 18104, USADepartment of Biology, Muhlenberg College, 2400 W. Chew Street, Allentown, PA 18104, USADepartment of Biology, Muhlenberg College, 2400 W. Chew Street, Allentown, PA 18104, USAThe objective of this study was to determine if the interaction between common oral commensal bacteria and oral epithelial cells would provide protective effects against the invasion of periodontopathogen <i>Porphyromonas gingivalis</i>. Oral epithelial OKF6/Tert cells were used in co-cultures with <i>Streptococcus gordonii</i>, <i>Streptococcus oralis</i>, <i>Streptococcus mitis</i>, and <i>Streptococcus intermedius</i>. The viability of OKF6/Tert cells following a bacterial challenge was evaluated by trypan blue exclusion. The adherence of commensal species was determined by CFU counts. <i>P. gingivalis</i> invasion in OKF6/Tert cells was assessed before and after exposure to commensal species according to CFU counts. Viability assays show that only <i>S. gordonii</i> and <i>S. intermedius</i> display low toxicity toward OKF6/Tert cells. Both commensals adhere to OKF6/Tert cells at an average ratio of 1 CFU to 10 cells. <i>P. gingivalis</i> invasion into host cells is significantly reduced by 25% or 60% after exposure to <i>S. gordonii</i> or <i>S. intermedius</i>, respectively. The results suggest that these commensal species bind to host cells and diminish <i>P. gingivalis</i> invasion. This is important in the context of periodontal disease since <i>P. gingivalis</i> primarily acts on the host by invading it. Therefore, efforts to decrease invasion will eventually lead to future therapies harnessing the mechanisms employed by oral commensal bacteria.https://www.mdpi.com/2304-6767/8/2/39oralcommensalstreptococci<i>P. gingivalis</i>invasionprotection |
| spellingShingle | Alyssa N. Hanel Hannah M. Herzog Michelle G. James Giancarlo A. Cuadra Effects of Oral Commensal Streptococci on <i>Porphyromonas gingivalis</i> Invasion into Oral Epithelial Cells Dentistry Journal oral commensal streptococci <i>P. gingivalis</i> invasion protection |
| title | Effects of Oral Commensal Streptococci on <i>Porphyromonas gingivalis</i> Invasion into Oral Epithelial Cells |
| title_full | Effects of Oral Commensal Streptococci on <i>Porphyromonas gingivalis</i> Invasion into Oral Epithelial Cells |
| title_fullStr | Effects of Oral Commensal Streptococci on <i>Porphyromonas gingivalis</i> Invasion into Oral Epithelial Cells |
| title_full_unstemmed | Effects of Oral Commensal Streptococci on <i>Porphyromonas gingivalis</i> Invasion into Oral Epithelial Cells |
| title_short | Effects of Oral Commensal Streptococci on <i>Porphyromonas gingivalis</i> Invasion into Oral Epithelial Cells |
| title_sort | effects of oral commensal streptococci on i porphyromonas gingivalis i invasion into oral epithelial cells |
| topic | oral commensal streptococci <i>P. gingivalis</i> invasion protection |
| url | https://www.mdpi.com/2304-6767/8/2/39 |
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