Nature of Hard Tissues in Oral Pathological Lesions –Using Modified Gallego’s Stain
Introduction: Histological stains are dyes that bind to various tissues. Special stains form an integral part of routine histopathology as an adjunct to Haematoxylin and Eosin (H&E), and give meaningful diagnostic information of the tissues available. By using routine histological procedure...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2017-04-01
|
| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/9566/25244_CE[Ra1]_F(RK)_PF1(VG_RK)_PFA(P)_PF2(P_SH).pdf |
| _version_ | 1811206633856958464 |
|---|---|
| author | Panthala Veenila Mudhiraj Manjushri Madhukar Vanje Bachamgari Narender Reddy Syed Afroz Ahmed Charu Suri Shahela Taveer Shravan Kumar |
| author_facet | Panthala Veenila Mudhiraj Manjushri Madhukar Vanje Bachamgari Narender Reddy Syed Afroz Ahmed Charu Suri Shahela Taveer Shravan Kumar |
| author_sort | Panthala Veenila Mudhiraj |
| collection | DOAJ |
| description | Introduction: Histological stains are dyes that bind to
various tissues. Special stains form an integral part of routine
histopathology as an adjunct to Haematoxylin and Eosin (H&E),
and give meaningful diagnostic information of the tissues
available. By using routine histological procedure like H&E alone,
it is difficult to differentiate the various hard tissues present in
the oral pathological lesions. Modified Gallego’s stain can be
used as one of the differential stain for these hard tissues.
Aim: To differentiate various hard tissues of teeth and to
identify the presence of hard tissue components in different oral
pathological lesions using Modified Gallego’s stain under light
microscope.
Materials and Methods: A total sample of 20 cases, amongst
which 10 were human extracted teeth and 10 oral pathological
lesions were included. From 10 human extracted teeth, five
ground sections and five decalcified sections were prepared.
From pathological lesions, two slides of each lesion were
prepared. All the sections were stained with Modified Gallego’s
stain and viewed under light microscopy.
Results: In properly stained slides, cementum stained red, dentin
and bone stained green and enamel stained pink in colour.
Conclusion: Modified Gallego’s stain can be used as a
differential stain for various hard tissues in oral pathological
lesions and also for ground sections for which histochemical
stains are very rare. |
| first_indexed | 2024-04-12T03:50:27Z |
| format | Article |
| id | doaj.art-1eb5800b69204094a8b1f737941dcd48 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-12T03:50:27Z |
| publishDate | 2017-04-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-1eb5800b69204094a8b1f737941dcd482022-12-22T03:48:59ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-04-01114ZC13ZC1510.7860/JCDR/2017/25244.9566Nature of Hard Tissues in Oral Pathological Lesions –Using Modified Gallego’s StainPanthala Veenila Mudhiraj0Manjushri Madhukar Vanje1Bachamgari Narender Reddy2Syed Afroz Ahmed3Charu Suri4Shahela Taveer5Shravan Kumar6Postgraduate, Department of Oral Pathology, Sri Sai College of Dental Surgery, Hyderabad, Telangana, India.Postgraduate, Department of Oral Pathology, Sri Sai College of Dental Surgery, Hyderabad, Telangana, India.Postgraduate, Department of Oral Pathology, Sri Sai College of Dental Surgery, Hyderabad, Telangana, India.Professor and Head of the Department, Department of Oral Pathology, Sri Sai College of Dental Surgery, Hyderabad, Telangana, India.Professor, Oral Pathology, Sri Sai College of Dental Surgery, Hyderabad, Telangana, India.Senior Lecturer, Department of Oral Pathology, Sri Sai College of Dental Surgery, Hyderabad, Telangana, India.Senior Lecturer, Department of Oral Pathology, Sri Sai College of Dental Surgery, Hyderabad, Telangana, India.Introduction: Histological stains are dyes that bind to various tissues. Special stains form an integral part of routine histopathology as an adjunct to Haematoxylin and Eosin (H&E), and give meaningful diagnostic information of the tissues available. By using routine histological procedure like H&E alone, it is difficult to differentiate the various hard tissues present in the oral pathological lesions. Modified Gallego’s stain can be used as one of the differential stain for these hard tissues. Aim: To differentiate various hard tissues of teeth and to identify the presence of hard tissue components in different oral pathological lesions using Modified Gallego’s stain under light microscope. Materials and Methods: A total sample of 20 cases, amongst which 10 were human extracted teeth and 10 oral pathological lesions were included. From 10 human extracted teeth, five ground sections and five decalcified sections were prepared. From pathological lesions, two slides of each lesion were prepared. All the sections were stained with Modified Gallego’s stain and viewed under light microscopy. Results: In properly stained slides, cementum stained red, dentin and bone stained green and enamel stained pink in colour. Conclusion: Modified Gallego’s stain can be used as a differential stain for various hard tissues in oral pathological lesions and also for ground sections for which histochemical stains are very rare.https://jcdr.net/articles/PDF/9566/25244_CE[Ra1]_F(RK)_PF1(VG_RK)_PFA(P)_PF2(P_SH).pdfdecalcified sectionsdifferential stainground sections |
| spellingShingle | Panthala Veenila Mudhiraj Manjushri Madhukar Vanje Bachamgari Narender Reddy Syed Afroz Ahmed Charu Suri Shahela Taveer Shravan Kumar Nature of Hard Tissues in Oral Pathological Lesions –Using Modified Gallego’s Stain Journal of Clinical and Diagnostic Research decalcified sections differential stain ground sections |
| title | Nature of Hard Tissues in Oral Pathological Lesions –Using Modified Gallego’s Stain |
| title_full | Nature of Hard Tissues in Oral Pathological Lesions –Using Modified Gallego’s Stain |
| title_fullStr | Nature of Hard Tissues in Oral Pathological Lesions –Using Modified Gallego’s Stain |
| title_full_unstemmed | Nature of Hard Tissues in Oral Pathological Lesions –Using Modified Gallego’s Stain |
| title_short | Nature of Hard Tissues in Oral Pathological Lesions –Using Modified Gallego’s Stain |
| title_sort | nature of hard tissues in oral pathological lesions using modified gallego s stain |
| topic | decalcified sections differential stain ground sections |
| url | https://jcdr.net/articles/PDF/9566/25244_CE[Ra1]_F(RK)_PF1(VG_RK)_PFA(P)_PF2(P_SH).pdf |
| work_keys_str_mv | AT panthalaveenilamudhiraj natureofhardtissuesinoralpathologicallesionsusingmodifiedgallegosstain AT manjushrimadhukarvanje natureofhardtissuesinoralpathologicallesionsusingmodifiedgallegosstain AT bachamgarinarenderreddy natureofhardtissuesinoralpathologicallesionsusingmodifiedgallegosstain AT syedafrozahmed natureofhardtissuesinoralpathologicallesionsusingmodifiedgallegosstain AT charusuri natureofhardtissuesinoralpathologicallesionsusingmodifiedgallegosstain AT shahelataveer natureofhardtissuesinoralpathologicallesionsusingmodifiedgallegosstain AT shravankumar natureofhardtissuesinoralpathologicallesionsusingmodifiedgallegosstain |