Leishmania infantum infecting the carnivore Nasua nasua from urban forest fragments in an endemic area of visceral leishmaniasis in Brazilian Midwest
IntroductionThe aim of the present study was to investigate the occurrence of Leishmania infantum in South American coatis inhabiting two forest fragments in Campo Grande, Mato Grosso do Sul, Midwest region of Brazil, an endemic area of human and canine visceral leishmaniasis (VL).Material and metho...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1050339/full |
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| author | Gabriel Carvalho de Macedo Wanessa Teixeira Gomes Barreto Carina Elisei de Oliveira Carina Elisei de Oliveira Filipe Martins Santos Grasiela Edith de Oliveira Porfírio Samanta Cristina das Chagas Xavier Fernanda Moreira Alves Fernanda Moreira Alves Alanderson Rodrigues da Silva Gisele Braziliano de Andrade Andreza Castro Rucco William Oliveira de Assis Ana Maria Jansen André Luiz Rodrigues Roque Heitor Miraglia Herrera Heitor Miraglia Herrera |
| author_facet | Gabriel Carvalho de Macedo Wanessa Teixeira Gomes Barreto Carina Elisei de Oliveira Carina Elisei de Oliveira Filipe Martins Santos Grasiela Edith de Oliveira Porfírio Samanta Cristina das Chagas Xavier Fernanda Moreira Alves Fernanda Moreira Alves Alanderson Rodrigues da Silva Gisele Braziliano de Andrade Andreza Castro Rucco William Oliveira de Assis Ana Maria Jansen André Luiz Rodrigues Roque Heitor Miraglia Herrera Heitor Miraglia Herrera |
| author_sort | Gabriel Carvalho de Macedo |
| collection | DOAJ |
| description | IntroductionThe aim of the present study was to investigate the occurrence of Leishmania infantum in South American coatis inhabiting two forest fragments in Campo Grande, Mato Grosso do Sul, Midwest region of Brazil, an endemic area of human and canine visceral leishmaniasis (VL).Material and methodsA total of 110 South American coatis were sampled in the conservation unit “Parque Estadual do Prosa” (PEP) and in the residential area “Vila da Base Aérea” (VBA) from March 2018 to April 2019. As a longitudinal study that include up to six recaptures of the same individual, a total of 190 capture events were obtained. Blood, bone marrow and skin samples were obtained for parasitological (axenic culture), serological (Enzyme Linked Immunosorbent Assay – ELISA and Dual-path Platform immunoassay – DPP® CVL) and molecular diagnostic assays (targeting kDNA for Leishmania spp. and L. infantum; and HSP70 followed by sequence analysis).ResultsSeropositivity for L. infantum was found in 33 individuals, six in PEP and 27 in VBA. Furthermore, L. infantum was detected by molecular analysis in 16 individuals, seven from PEP and nine from VBA. We also isolated L. infantum from bone marrow of one individual and detected a single positive skin sample in molecular assay from other individual, both from VBA.DiscussionAn overall infection rate of 36.4% (40/110) was observed, significantly higher in the VBA (49.1%) than in the PEP (21.6%), probably because VBA presents: (i) a large number of resident dogs and chickens that would be attracting sandflies; (ii) a denser population of this wild mammal species; and (iii) physical barriers and a lack of functional connectivity in the surroundings, preventing these animals to disperse out. We conclude that South American coati populations living in urban forest fragments of Campo Grande are affected by the epidemiological scenario of VL, known to involve dogs, vectors and humans. We highlight the importance of investigate the parasitism by L. infantum in this and other potential L. infantum reservoirs that inhabit urbanized regions endemic to VL. |
| first_indexed | 2024-04-10T05:56:59Z |
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| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-10T05:56:59Z |
| publishDate | 2023-01-01 |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-1eba7c204c184c13aa691e8aa2986d942023-03-03T10:52:02ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-01-011210.3389/fcimb.2022.10503391050339Leishmania infantum infecting the carnivore Nasua nasua from urban forest fragments in an endemic area of visceral leishmaniasis in Brazilian MidwestGabriel Carvalho de Macedo0Wanessa Teixeira Gomes Barreto1Carina Elisei de Oliveira2Carina Elisei de Oliveira3Filipe Martins Santos4Grasiela Edith de Oliveira Porfírio5Samanta Cristina das Chagas Xavier6Fernanda Moreira Alves7Fernanda Moreira Alves8Alanderson Rodrigues da Silva9Gisele Braziliano de Andrade10Andreza Castro Rucco11William Oliveira de Assis12Ana Maria Jansen13André Luiz Rodrigues Roque14Heitor Miraglia Herrera15Heitor Miraglia Herrera16Post-Graduate Program in Environmental Sciences and Agricultural Sustainability, Dom Bosco Catholic University, Campo Grande, BrazilPost-Graduate Program in Environmental Sciences and Agricultural Sustainability, Dom Bosco Catholic University, Campo Grande, BrazilPost-Graduate Program in Environmental Sciences and Agricultural Sustainability, Dom Bosco Catholic University, Campo Grande, BrazilPost-Graduate Program in Biotechnology, Dom Bosco Catholic University, Campo Grande, BrazilPost-Graduate Program in Environmental Sciences and Agricultural Sustainability, Dom Bosco Catholic University, Campo Grande, BrazilPost-Graduate Program in Environmental Sciences and Agricultural Sustainability, Dom Bosco Catholic University, Campo Grande, BrazilLaboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, BrazilLaboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, BrazilPost-Graduate Program in Parasite Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, BrazilPost-Graduate Program in Biotechnology, Dom Bosco Catholic University, Campo Grande, BrazilPost-Graduate Program in Environmental Sciences and Agricultural Sustainability, Dom Bosco Catholic University, Campo Grande, BrazilPost-Graduate Program in Environmental Sciences and Agricultural Sustainability, Dom Bosco Catholic University, Campo Grande, BrazilPost-Graduate Program in Environmental Sciences and Agricultural Sustainability, Dom Bosco Catholic University, Campo Grande, BrazilLaboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, BrazilLaboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, BrazilPost-Graduate Program in Environmental Sciences and Agricultural Sustainability, Dom Bosco Catholic University, Campo Grande, BrazilPost-Graduate Program in Biotechnology, Dom Bosco Catholic University, Campo Grande, BrazilIntroductionThe aim of the present study was to investigate the occurrence of Leishmania infantum in South American coatis inhabiting two forest fragments in Campo Grande, Mato Grosso do Sul, Midwest region of Brazil, an endemic area of human and canine visceral leishmaniasis (VL).Material and methodsA total of 110 South American coatis were sampled in the conservation unit “Parque Estadual do Prosa” (PEP) and in the residential area “Vila da Base Aérea” (VBA) from March 2018 to April 2019. As a longitudinal study that include up to six recaptures of the same individual, a total of 190 capture events were obtained. Blood, bone marrow and skin samples were obtained for parasitological (axenic culture), serological (Enzyme Linked Immunosorbent Assay – ELISA and Dual-path Platform immunoassay – DPP® CVL) and molecular diagnostic assays (targeting kDNA for Leishmania spp. and L. infantum; and HSP70 followed by sequence analysis).ResultsSeropositivity for L. infantum was found in 33 individuals, six in PEP and 27 in VBA. Furthermore, L. infantum was detected by molecular analysis in 16 individuals, seven from PEP and nine from VBA. We also isolated L. infantum from bone marrow of one individual and detected a single positive skin sample in molecular assay from other individual, both from VBA.DiscussionAn overall infection rate of 36.4% (40/110) was observed, significantly higher in the VBA (49.1%) than in the PEP (21.6%), probably because VBA presents: (i) a large number of resident dogs and chickens that would be attracting sandflies; (ii) a denser population of this wild mammal species; and (iii) physical barriers and a lack of functional connectivity in the surroundings, preventing these animals to disperse out. We conclude that South American coati populations living in urban forest fragments of Campo Grande are affected by the epidemiological scenario of VL, known to involve dogs, vectors and humans. We highlight the importance of investigate the parasitism by L. infantum in this and other potential L. infantum reservoirs that inhabit urbanized regions endemic to VL.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1050339/fulllongitudinal studySouth American coatiLeishmania infantumurban faunavisceral leishmaniasis |
| spellingShingle | Gabriel Carvalho de Macedo Wanessa Teixeira Gomes Barreto Carina Elisei de Oliveira Carina Elisei de Oliveira Filipe Martins Santos Grasiela Edith de Oliveira Porfírio Samanta Cristina das Chagas Xavier Fernanda Moreira Alves Fernanda Moreira Alves Alanderson Rodrigues da Silva Gisele Braziliano de Andrade Andreza Castro Rucco William Oliveira de Assis Ana Maria Jansen André Luiz Rodrigues Roque Heitor Miraglia Herrera Heitor Miraglia Herrera Leishmania infantum infecting the carnivore Nasua nasua from urban forest fragments in an endemic area of visceral leishmaniasis in Brazilian Midwest Frontiers in Cellular and Infection Microbiology longitudinal study South American coati Leishmania infantum urban fauna visceral leishmaniasis |
| title | Leishmania infantum infecting the carnivore Nasua nasua from urban forest fragments in an endemic area of visceral leishmaniasis in Brazilian Midwest |
| title_full | Leishmania infantum infecting the carnivore Nasua nasua from urban forest fragments in an endemic area of visceral leishmaniasis in Brazilian Midwest |
| title_fullStr | Leishmania infantum infecting the carnivore Nasua nasua from urban forest fragments in an endemic area of visceral leishmaniasis in Brazilian Midwest |
| title_full_unstemmed | Leishmania infantum infecting the carnivore Nasua nasua from urban forest fragments in an endemic area of visceral leishmaniasis in Brazilian Midwest |
| title_short | Leishmania infantum infecting the carnivore Nasua nasua from urban forest fragments in an endemic area of visceral leishmaniasis in Brazilian Midwest |
| title_sort | leishmania infantum infecting the carnivore nasua nasua from urban forest fragments in an endemic area of visceral leishmaniasis in brazilian midwest |
| topic | longitudinal study South American coati Leishmania infantum urban fauna visceral leishmaniasis |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1050339/full |
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