Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks
ABSTRACT: The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers. Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122007349 |
| _version_ | 1797854314296770560 |
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| author | K.D. Teague G. Tellez-Isaias Jianmin Chai V. Petrone-Garcia C.N. Vuong A. Blanch S.H. Rasmussen K. Brown Jiangchao Zhao S.J. Rochell |
| author_facet | K.D. Teague G. Tellez-Isaias Jianmin Chai V. Petrone-Garcia C.N. Vuong A. Blanch S.H. Rasmussen K. Brown Jiangchao Zhao S.J. Rochell |
| author_sort | K.D. Teague |
| collection | DOAJ |
| description | ABSTRACT: The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers. Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch. These 5 treatments were administered to 10 replicate cages of 8 birds. Performance was measured weekly and excreta moisture, N retention, apparent metabolizeable energy, and complete blood cell counts were determined at 14 and 21 d. At 21 d, 2 birds per cage were orally gavaged with fluorescein isothiocyanate-dextran (FITC-d) and serum samples were analyzed for FITC-d as a marker of gut leakage. Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically. Feed conversion ratio increased (P < 0.01) linearly as GOS increased. There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn. There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased. At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased. Serum concentrations of FITC-d increased quadratically (P < 0.01) to dietary GOS. Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium. Results from this trial indicate that soy-derived GOS exert dose-dependent effects on nutrient utilization and intestinal health in young broilers. |
| first_indexed | 2024-04-09T20:05:03Z |
| format | Article |
| id | doaj.art-1eba9212fb714a1fac9889ae8d8fada1 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-09T20:05:03Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-1eba9212fb714a1fac9889ae8d8fada12023-04-02T06:10:40ZengElsevierPoultry Science0032-57912023-04-011024102440Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicksK.D. Teague0G. Tellez-Isaias1Jianmin Chai2V. Petrone-Garcia3C.N. Vuong4A. Blanch5S.H. Rasmussen6K. Brown7Jiangchao Zhao8S.J. Rochell9Division of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville, AR, USADivision of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville, AR, USADivision of Agriculture, Department of Animal Science, University of Arkansas, Fayetteville, AR, USA; Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, School of Life Science and Engineering, Foshan University, Foshan, ChinaFaculty of Higher Studies Cuautitlan, National Autonomous University of Mexico, Cuautitlan Izcalli, State of Mexico, MexicoDivision of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville, AR, USAHamlet Protein A/S, DK-8700 Horsens, DenmarkHamlet Protein A/S, DK-8700 Horsens, DenmarkHamlet Protein A/S, DK-8700 Horsens, DenmarkDivision of Agriculture, Department of Animal Science, University of Arkansas, Fayetteville, AR, USADivision of Agriculture, Department of Poultry Science, University of Arkansas, Fayetteville, AR, USA; Department of Poultry Science, Auburn University, Auburn, AL, USA; Corresponding author:ABSTRACT: The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers. Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch. These 5 treatments were administered to 10 replicate cages of 8 birds. Performance was measured weekly and excreta moisture, N retention, apparent metabolizeable energy, and complete blood cell counts were determined at 14 and 21 d. At 21 d, 2 birds per cage were orally gavaged with fluorescein isothiocyanate-dextran (FITC-d) and serum samples were analyzed for FITC-d as a marker of gut leakage. Additionally, intestinal morphology, crop presumptive lactic acid bacteria (LAB) counts, crop and cecal pH, and cecal microbiota via16S rRNA microbial sequencing were evaluated at 21 d. From 0 to 21 d, feed intake increased linearly (P < 0.01) as dietary GOS increased, whereas BWG increased (P < 0.05) quadratically. Feed conversion ratio increased (P < 0.01) linearly as GOS increased. There were linear increases (P < 0.05) in excreta moisture as dietary GOS increased at 14 and 21 d, as well as dose-dependent responses (P < 0.05) in N retention, AME, and AMEn. There was a quadratic increase (P < 0.05) in crop LAB recovery and a linear decrease (P < 0.01) in ceca pH as GOS increased. At 14 d, a linear increase (P < 0.05) in blood heterophil to lymphocyte ratio was observed as dietary GOS increased. Serum concentrations of FITC-d increased quadratically (P < 0.01) to dietary GOS. Increasing levels of GOS influenced alpha and beta diversities and composition of gut microbiota, including the abundance of Ruminococcus and Bifidobacterium. Results from this trial indicate that soy-derived GOS exert dose-dependent effects on nutrient utilization and intestinal health in young broilers.http://www.sciencedirect.com/science/article/pii/S0032579122007349soyoligosaccharideraffinosestachyosebroiler |
| spellingShingle | K.D. Teague G. Tellez-Isaias Jianmin Chai V. Petrone-Garcia C.N. Vuong A. Blanch S.H. Rasmussen K. Brown Jiangchao Zhao S.J. Rochell Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks Poultry Science soy oligosaccharide raffinose stachyose broiler |
| title | Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks |
| title_full | Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks |
| title_fullStr | Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks |
| title_full_unstemmed | Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks |
| title_short | Dietary soy galactooligosaccharides affect the performance, intestinal function, and gut microbiota composition of growing chicks |
| title_sort | dietary soy galactooligosaccharides affect the performance intestinal function and gut microbiota composition of growing chicks |
| topic | soy oligosaccharide raffinose stachyose broiler |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122007349 |
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