Oral lactoferrin administration does not impact the diversity or composition of the infant gut microbiota in a Peruvian cohort
ABSTRACT Lactoferrin is a protein from breast milk involved in early-life microbiome assembly. It is believed to promote the growth of lactic acid-producing bacteria and inhibit pathogens by limiting iron availability and enabling an acidic environment in the gut. Previous reports suggest that oral...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2023-12-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.00096-23 |
| _version_ | 1827586724867342336 |
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| author | Luis González Jose Luis Paredes Sosa Susan Mosquito Yesenia Filio Pedro E. Romero Theresa J. Ochoa Pablo Tsukayama |
| author_facet | Luis González Jose Luis Paredes Sosa Susan Mosquito Yesenia Filio Pedro E. Romero Theresa J. Ochoa Pablo Tsukayama |
| author_sort | Luis González |
| collection | DOAJ |
| description | ABSTRACT Lactoferrin is a protein from breast milk involved in early-life microbiome assembly. It is believed to promote the growth of lactic acid-producing bacteria and inhibit pathogens by limiting iron availability and enabling an acidic environment in the gut. Previous reports suggest that oral administration of lactoferrin in neonates promotes the increase of gut microbiota diversity and the enrichment of beneficial microbes. However, its effect on infant gut microbiota over time has yet to be thoroughly studied to fully understand its potential as a prebiotic. Using 16s rRNA gene amplicon sequencing, we analyzed the gut microbiota composition of 60 toddlers at 12–18 months of age from Lima, Peru, who received daily oral administration of lactoferrin or placebo for 6 months since enrollment. Samples were analyzed at 0, 2, and 4 months after beginning treatment. Our results show that lactoferrin treatment does not increase gut microbiota diversity over time nor affect its bacterial composition compared to the placebo group. IMPORTANCE Previous studies have suggested that oral lactoferrin enhances diversity in the gut microbiota in infants while inhibiting the growth of opportunistic pathogens. However, the effect of lactoferrin on infant gut microbiota over time has yet to be thoroughly studied. Our study suggests that lactoferrin oral treatment in infants aged 12–18 months does not affect gut microbiome diversity and composition over time. To our knowledge, this is the first study to report the effect of lactoferrin on infant gut microbiome composition over time and helps elucidate its impact on infant health and its therapeutic potential. |
| first_indexed | 2024-03-09T00:05:54Z |
| format | Article |
| id | doaj.art-1ec1294fb5f74bd78f11a6970cd8bc8a |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-09T00:05:54Z |
| publishDate | 2023-12-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-1ec1294fb5f74bd78f11a6970cd8bc8a2023-12-12T13:17:19ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972023-12-0111610.1128/spectrum.00096-23Oral lactoferrin administration does not impact the diversity or composition of the infant gut microbiota in a Peruvian cohortLuis González0Jose Luis Paredes Sosa1Susan Mosquito2Yesenia Filio3Pedro E. Romero4Theresa J. Ochoa5Pablo Tsukayama6Laboratorio de Genómica Microbiana, Facultad de Ciencias e Ingeniería, Universidad Peruana Cayetano Heredia , Lima, PeruLaboratorio de Genómica Microbiana, Facultad de Ciencias e Ingeniería, Universidad Peruana Cayetano Heredia , Lima, PeruLaboratorio de Genómica Microbiana, Facultad de Ciencias e Ingeniería, Universidad Peruana Cayetano Heredia , Lima, PeruLaboratorio de Genómica Microbiana, Facultad de Ciencias e Ingeniería, Universidad Peruana Cayetano Heredia , Lima, PeruFacultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos , Lima, PeruInstituto de Medicina Tropical Alexander von Humboldt , Lima, PeruLaboratorio de Genómica Microbiana, Facultad de Ciencias e Ingeniería, Universidad Peruana Cayetano Heredia , Lima, PeruABSTRACT Lactoferrin is a protein from breast milk involved in early-life microbiome assembly. It is believed to promote the growth of lactic acid-producing bacteria and inhibit pathogens by limiting iron availability and enabling an acidic environment in the gut. Previous reports suggest that oral administration of lactoferrin in neonates promotes the increase of gut microbiota diversity and the enrichment of beneficial microbes. However, its effect on infant gut microbiota over time has yet to be thoroughly studied to fully understand its potential as a prebiotic. Using 16s rRNA gene amplicon sequencing, we analyzed the gut microbiota composition of 60 toddlers at 12–18 months of age from Lima, Peru, who received daily oral administration of lactoferrin or placebo for 6 months since enrollment. Samples were analyzed at 0, 2, and 4 months after beginning treatment. Our results show that lactoferrin treatment does not increase gut microbiota diversity over time nor affect its bacterial composition compared to the placebo group. IMPORTANCE Previous studies have suggested that oral lactoferrin enhances diversity in the gut microbiota in infants while inhibiting the growth of opportunistic pathogens. However, the effect of lactoferrin on infant gut microbiota over time has yet to be thoroughly studied. Our study suggests that lactoferrin oral treatment in infants aged 12–18 months does not affect gut microbiome diversity and composition over time. To our knowledge, this is the first study to report the effect of lactoferrin on infant gut microbiome composition over time and helps elucidate its impact on infant health and its therapeutic potential.https://journals.asm.org/doi/10.1128/spectrum.00096-23lactoferrinearly life microbiome16S rRNA sequencingPerugut microbiota |
| spellingShingle | Luis González Jose Luis Paredes Sosa Susan Mosquito Yesenia Filio Pedro E. Romero Theresa J. Ochoa Pablo Tsukayama Oral lactoferrin administration does not impact the diversity or composition of the infant gut microbiota in a Peruvian cohort Microbiology Spectrum lactoferrin early life microbiome 16S rRNA sequencing Peru gut microbiota |
| title | Oral lactoferrin administration does not impact the diversity or composition of the infant gut microbiota in a Peruvian cohort |
| title_full | Oral lactoferrin administration does not impact the diversity or composition of the infant gut microbiota in a Peruvian cohort |
| title_fullStr | Oral lactoferrin administration does not impact the diversity or composition of the infant gut microbiota in a Peruvian cohort |
| title_full_unstemmed | Oral lactoferrin administration does not impact the diversity or composition of the infant gut microbiota in a Peruvian cohort |
| title_short | Oral lactoferrin administration does not impact the diversity or composition of the infant gut microbiota in a Peruvian cohort |
| title_sort | oral lactoferrin administration does not impact the diversity or composition of the infant gut microbiota in a peruvian cohort |
| topic | lactoferrin early life microbiome 16S rRNA sequencing Peru gut microbiota |
| url | https://journals.asm.org/doi/10.1128/spectrum.00096-23 |
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